Shuimoliuyun (Talk | contribs) |
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<h3 style="text-align:center">Result</h3> | <h3 style="text-align:center">Result</h3> | ||
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We first selected the mutations within the 5Å distance of active site, altogether 33 kinds, and then used point mutation to conduct molecular cloning operation. Next step was to synthesize the acrylic acid using the whole cell catalysis and determined the acrylic acid yield by HPLC. The results are as follows: <br> | We first selected the mutations within the 5Å distance of active site, altogether 33 kinds, and then used point mutation to conduct molecular cloning operation. Next step was to synthesize the acrylic acid using the whole cell catalysis and determined the acrylic acid yield by HPLC. The results are as follows: <br> | ||
<center> <img src="https://static.igem.org/mediawiki/2017/f/fd/NPU-image06.png" class="img-responsive"></center> | <center> <img src="https://static.igem.org/mediawiki/2017/f/fd/NPU-image06.png" class="img-responsive"></center> |
Revision as of 19:53, 1 November 2017