When building a bacterial system meant to function in a more complex setting than in bulk in a test tube, modeling what would its behavior be in real conditions is key to aim for a credible development. Because the experimental conditions that were available to us to develop our system (in vitro experiments) were quite far from the actual situation (in vivo tumor colonization), a significant amount of work on the modeling was needed, particularly to implement the Tumor Sensor

With our model, we could make the link between what was happening during our in vitro experiments and what would correspondingly happen in the real case scenario in the tumor.

A thermal diffusion model also helped us take a crucial decision about the thermal induction temperature of our Heat Sensor, validating the relevance of choosing a higher temperature to limit the leakiness of our optimized part, but still within a safe range compatible with our clinical application.