Difference between revisions of "Team:TecCEM/Demonstrate"

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                 <p></br>The following table shows the values for each point using the equation.</br></br></p>
 
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<th> X</th>
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<th >Xm </th>
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<th >T-Xm </th>
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<tr">
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<th> 0 </th>
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<th >1 </th>
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<th >1 </th>
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<tr  ">
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<th> 20</th>
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<th> 0.9 </th>
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<th >0.97992</th>
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</tr>
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<tr">
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<th> 40</th>
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<th> 0.6</th>
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<th> 0.632184</th>
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</tr>
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<tr">
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<th> 60 </th>
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<th> 0.57 </th>
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<th> 0.285714 </th>
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 +
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</tr>
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<tr">
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<th> 100</th>
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<th> 0.25</th>
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<th> 0.177328</th>
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</tr>
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<table>
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Revision as of 21:12, 1 November 2017

IGEM_TECCEM

Demonstrate

Demonstrate

Math modeling with our results


We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The d and θ were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.


The following table shows the values for each point using the equation.

X Xm T-Xm
0 1 1
20 0.9 0.97992
40 0.6 0.632184
60 0.57 0.285714
100 0.25 0.177328

siRNA confirmation


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IGEM_TECCEM