Difference between revisions of "Team:Fudan/Basic Part"

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</p>
 
</p>
 
</div>
 
</div>
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<table>
 
<table>
 
   <tr>
 
   <tr>
−
     <td>2_ZF42-10/43-8 binding site</td>
+
     <th>2_ZF42-10/43-8 binding site</th>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446001”>(BBa_K2446001)</a></td>
+
     <th><partinfo>BBa_K2446001</partinfo></th>
 
   </tr>
 
   </tr>
−
 
 
   <tr>
 
   <tr>
 
     <td>2_ZF54-8/43-8 binding site</td>
 
     <td>2_ZF54-8/43-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446002”>(BBa_K2446002)</a></td>
+
     <td><partinfo>BBa_K2446002</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>2_ZFHD1 binding site</td>
 
     <td>2_ZFHD1 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446003”>(BBa_K2446003)</a></td>
+
     <td><partinfo>BBa_K2446003</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>2_PIP binding site</td>
 
     <td>2_PIP binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446004”>(BBa_K2446004)</a></td>
+
     <td><partinfo>BBa_K2446004</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZF_42-10 binding site</td>
 
     <td>4_ZF_42-10 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446005”>(BBa_K2446005)</a></td>
+
     <td><partinfo>BBa_K2446005</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZF_43-8 binding site</td>
 
     <td>4_ZF_43-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446006”>(BBa_K2446006)</a></td>
+
     <td><partinfo>BBa_K2446006</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZF_54-8 binding site</td>
 
     <td>4_ZF_54-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446007”>(BBa_K2446007)</a></td>
+
     <td><partinfo>BBa_K2446007</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZF_21-16 binding site</td>
 
     <td>4_ZF_21-16 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446008”>(BBa_K2446008)</a></td>
+
     <td><partinfo>BBa_K2446008</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZF54-8/43-8 binding site</td>
 
     <td>4_ZF54-8/43-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446009”>(BBa_K2446009)</a></td>
+
     <td><partinfo>BBa_K2446009</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_ZFHD1 binding site</td>
 
     <td>4_ZFHD1 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446010”>(BBa_K2446010)</a></td>
+
     <td><partinfo>BBa_K2446010</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>4_PIP binding site</td>
 
     <td>4_PIP binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446011”>(BBa_K2446011)</a></td>
+
     <td><partinfo>BBa_K2446011</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_ZF_42-10 binding site</td>
 
     <td>8_ZF_42-10 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446012”>(BBa_K2446012)</a></td>
+
     <td><partinfo>BBa_K2446012</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_ZF_43-8 binding site</td>
 
     <td>8_ZF_43-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446013”>(BBa_K2446013)</a></td>
+
     <td><partinfo>BBa_K2446013</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_ZF_54-8 binding site</td>
 
     <td>8_ZF_54-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446014”>(BBa_K2446014)</a></td>
+
     <td><partinfo>BBa_K2446014</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_ZF_21-16 binding site</td>
 
     <td>8_ZF_21-16 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446015”>(BBa_K2446015)</a></td>
+
     <td><partinfo>BBa_K2446015</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_ZF_54-8/43-8 binding site</td>
 
     <td>8_ZF_54-8/43-8 binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446016”>(BBa_K2446016)</a></td>
+
     <td><partinfo>BBa_K2446016</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>8_PIP binding site</td>
 
     <td>8_PIP binding site</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446017”>(BBa_K2446017)</a></td>
+
     <td><partinfo>BBa_K2446017</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_GAl4_KRAB</td>
 
     <td>ZF_GAl4_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446037”>(BBa_K2446037)</a></td>
+
     <td><partinfo>BBa_K2446037</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_PIP_KRAB</td>
 
     <td>ZF_PIP_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446038”>(BBa_K2446038)</a></td>
+
     <td><partinfo>BBa_K2446038</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_21-16KRAB</td>
 
     <td>ZF_21-16KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446039”>(BBa_K2446039)</a></td>
+
     <td><partinfo>BBa_K2446039</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_42-10_KRAB</td>
 
     <td>ZF_42-10_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446040”>(BBa_K2446040)</a></td>
+
     <td><partinfo>BBa_K2446040</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_43-8_KRAB</td>
 
     <td>ZF_43-8_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446041”>(BBa_K2446041)</a></td>
+
     <td><partinfo>BBa_K2446041</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZF_54-8_KRAB</td>
 
     <td>ZF_54-8_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446042”>(BBa_K2446042)</a></td>
+
     <td><partinfo>BBa_K2446042</partinfo></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>ZFHD1_KRAB</td>
 
     <td>ZFHD1_KRAB</td>
−
     <td><a href=“http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446043”>(BBa_K2446043)</a></td>
+
     <td><partinfo>BBa_K2446043</partinfo></td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
 
  
  

Revision as of 21:34, 1 November 2017
Basic Part – surEGFP


For this year’s iGEM competition, our favorite basic part is surEGFP
(BBa_K2446051)


This part is a surface-expressed EGFP which can be used to set up heterogeneous antigen density expression cell model. Our team is very excited to be the first to bring the idea of antigen density heterogeneity to the iGEM competition, and propose a tri-response immunotherapy, SwordS.



How surEGFP works

Surface-expressed EGFP (surEGFP) is built by fusing CD8α signal peptid and EGFP to the transmembrane region of CD86 (NM_175862) from N terminal to C terminal. CD8α peptide guides surEGFP to be located to cell membrane. transmembrane region of CD86 supports surEGFP to fix on membrane.



See More

Integrated information about surEGFP posted on Demonstrate page: Antigen density heterogeneity cell model. Or you can alternatively check the (BBa_K2446051) on the Registry of Standard Biological Parts website.

We listed other basic part we constructed this year here.

2_ZF42-10/43-8 binding site BBa_K2446001
2_ZF54-8/43-8 binding site BBa_K2446002
2_ZFHD1 binding site BBa_K2446003
2_PIP binding site BBa_K2446004
4_ZF_42-10 binding site BBa_K2446005
4_ZF_43-8 binding site BBa_K2446006
4_ZF_54-8 binding site BBa_K2446007
4_ZF_21-16 binding site BBa_K2446008
4_ZF54-8/43-8 binding site BBa_K2446009
4_ZFHD1 binding site BBa_K2446010
4_PIP binding site BBa_K2446011
8_ZF_42-10 binding site BBa_K2446012
8_ZF_43-8 binding site BBa_K2446013
8_ZF_54-8 binding site BBa_K2446014
8_ZF_21-16 binding site BBa_K2446015
8_ZF_54-8/43-8 binding site BBa_K2446016
8_PIP binding site BBa_K2446017
ZF_GAl4_KRAB BBa_K2446037
ZF_PIP_KRAB BBa_K2446038
ZF_21-16KRAB BBa_K2446039
ZF_42-10_KRAB BBa_K2446040
ZF_43-8_KRAB BBa_K2446041
ZF_54-8_KRAB BBa_K2446042
ZFHD1_KRAB BBa_K2446043