Team:Fudan/Composite Part
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This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.
We listed other composite part we constructed this year below.
| SV40_8_ZF_42-10 promoter | (BBa_K2446025) |
| SV40_2_ZF_43-8 promoter | (BBa_K2446026) |
| SV40_4_ZF_43-8 promoter | (BBa_K2446027) |
| SV40_8_ZF_54-8 promoter | (BBa_K2446029) |
| SV40_8_ZF_21-16 promoter | (BBa_K2446030) |
| SV40_8_ZF54-8/43-8 promoter | (BBa_K2446031) |
| SV40_4_ZFHD1 promoter | (BBa_K2446032) |
| SV40_2_PIP promoter | (BBa_K2446033) |
| SV40_4_PIP promoter | (BBa_K2446034) |
| SV40_8_PIP promoter | (BBa_K2446035) |
| SV40_UAS promoter | (BBa_K2446036) |
| CMV_8_ZF_42-10 promoter | (BBa_K2446018) |
| CMV_8_ZF_43-8 promoter | (BBa_K2446019) |
| CMV_8_ZF_54-8 promoter | (BBa_K2446020) |
| CMV_8_ZF54-8/43-8 promoter | (BBa_K2446021) |
| CMV_4_ZFHD1 promoter | (BBa_K2446022) |
| CMV_4_PIP promoter | (BBa_K2446023) |
| CMV_8_PIP promoter | (BBa_K2446024) |
