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| | </div> | | </div> |
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| − |
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| − | <table>
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| − | <tr>
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| − | <td>SV40_8_ZF_42-10 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446025”>(BBa_K2446025)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_2_ZF_43-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446026”>(BBa_K2446026)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_4_ZF_43-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446027”>(BBa_K2446027)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_8_ZF_54-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446029”>(BBa_K2446029)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_8_ZF_21-16 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446030”>(BBa_K2446030)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_8_ZF54-8/43-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446031”>(BBa_K2446031)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_4_ZFHD1 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446032”>(BBa_K2446032)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_2_PIP promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446033”>(BBa_K2446033)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_4_PIP promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446034”>(BBa_K2446034)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_8_PIP promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446035”>(BBa_K2446035)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>SV40_UAS promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446036”>(BBa_K2446036)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_8_ZF_42-10 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446018”>(BBa_K2446018)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_8_ZF_43-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446019”>(BBa_K2446019)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_8_ZF_54-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446020”>(BBa_K2446020)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_8_ZF54-8/43-8 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446021”>(BBa_K2446021)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_4_ZFHD1 promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446022”>(BBa_K2446022)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_4_PIP promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446023”>(BBa_K2446023)</a></td>
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| − | </tr>
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| − | <tr>
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| − | <td>CMV_8_PIP promoter</td>
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| − | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446024”>(BBa_K2446024)</a></td>
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| − | </tr>
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| − | </table>
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| | | | |
| | | | |
Composite part – SynPro(S)-ZF43-8;8*
For this year’s iGEM competition, our favorite basic part is SynPro(S)-43-8;8* (BBa_K2446028).
This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
How pSV40-ZF43-8;8*works
The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ end to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
The advantages of pSV40-ZF43-8;8*
Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression in constant expression state is high. Meanwhile, it is almost being completely silenced in silencing expression state.
The silencing fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
See More
Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.
We listed all the composite parts we constructed this year here.
