Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Methods"
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<ul style="list-style-type: none"> | <ul style="list-style-type: none"> | ||
<li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/6/61/T--Bielefeld-CeBiTec--CMZ-Acetone.pdf" target="blank">Acetone Precipitation</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/e/e8/T--Bielefeld-CeBiTec--protocol_biotinylierung_of_glass_slides.pdf" target="blank">Biotinylation of glass slides</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/e/e8/T--Bielefeld-CeBiTec--protocol_biotinylierung_of_glass_slides.pdf" target="blank">Biotinylation of glass slides</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/9/97/T--Bielefeld-CeBiTec--protocol_cloning_strategy_re.pdf" target="blank">Cloning Strategy with Restriction Enzymes</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/9/97/T--Bielefeld-CeBiTec--protocol_cloning_strategy_re.pdf" target="blank">Cloning Strategy with Restriction Enzymes</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/2/25/T--Bielefeld-CeBiTec--CMZ-FastcellSDS.pdf" target="blank">Fast cell lysis sample buffer</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/e/e0/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_SDS-Page.pdf" target="blank">Fast cell lysis for SDS-PAGE</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/e/e0/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_SDS-Page.pdf" target="blank">Fast cell lysis for SDS-PAGE</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/3/39/T--Bielefeld-CeBiTec--protocol_GeneJET_DNA_Purification_Thermo.pdf.pdf" target="blank">GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/3/39/T--Bielefeld-CeBiTec--protocol_GeneJET_DNA_Purification_Thermo.pdf.pdf" target="blank">GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)</a></li> | ||
| Line 50: | Line 50: | ||
<li><a href="https://static.igem.org/mediawiki/2017/6/62/T--Bielefeld-CeBiTec--CMZ-PBJRbuffer.pdf" target="blank">PBJR buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/6/62/T--Bielefeld-CeBiTec--CMZ-PBJRbuffer.pdf" target="blank">PBJR buffer</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/2/2b/T--Bielefeld-CeBiTec--ProtocolPhusion.pdf" target="blank">PCR with Phusion High Fidelity Master Mix with HC enhancer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/2/2b/T--Bielefeld-CeBiTec--ProtocolPhusion.pdf" target="blank">PCR with Phusion High Fidelity Master Mix with HC enhancer</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/1/16/T--Bielefeld-CeBiTec--CMZ-PCRQ5.pdf" target="blank">PCR with Q5 High-Fidelity Polymerase</a></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/e/ec/T--Bielefeld-CeBiTec--CMZ-GoldenGate.pdf" target="blank">Plasmid Assembly Protocol with Golden Gate Assembly</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--ImpactProtocol.pdf">Protein Purification Impact-CN Protocol</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--ImpactProtocol.pdf">Protein Purification Impact-CN Protocol</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/9/96/T--Bielefeld-CeBiTec--protocol_PCR_PureYield_PlasmidMiniprep_Promega.pdf" target="blank">PureYield<sup>TM</sup> Plasmid Miniprep System (Promega)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/9/96/T--Bielefeld-CeBiTec--protocol_PCR_PureYield_PlasmidMiniprep_Promega.pdf" target="blank">PureYield<sup>TM</sup> Plasmid Miniprep System (Promega)</a></li> | ||
Revision as of 22:30, 1 November 2017
Methods
Protocols
This page lists all regular methods used during our project. Special methods, which are not listed here, are described or linked on the corresponding result pages.
- 50xTAE Stock Solution
- Acetone Precipitation
- Biotinylation of glass slides
- Blunt end ligation
- Roti_Nanoquant (for protein concentration estimation)
- Cloning Strategy with Restriction Enzymes
- Colony PCR GoTaq® G2 DNA Polymerase
- Fast cell lysis sample buffer
- Fast cell lysis for SDS-PAGE
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- Generating chemocompetent cells
- Generating electrocompetent cells
- Genome editing via the CRISP-Cas9 system
- Gibson Assembly
- innuPREP Gel Extraktion Kit (Analytik Jena)
- Lycopene extraction
- Lysogeny Broth (LB)
- Monarch® Plasmid Miniprep Kit (NEB)
- Monarch® PCR & DNA Cleanup Kit (NEB)
- MOPS Minimal Medium
- Native DNA PAGE
- PBJR buffer
- PCR with Phusion High Fidelity Master Mix with HC enhancer
- PCR with Q5 High-Fidelity Polymerase
- Plasmid Assembly Protocol with Golden Gate Assembly
- Protein Purification Impact-CN Protocol
- PureYieldTM Plasmid Miniprep System (Promega)
- SDS-PAGE
- SDS-PAGE running buffer
- SiO2 Column Chromatography with Gradient
- SOC-medium
- ssDNA Annealing
- Standard BioBrick Assembly
- Synthesis of CBT-asparagine
- TAE buffer
- TLC analysis
- Transformation via electroporation
- Transformation via heat shock
- "Typical" Restriction Digest
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- ZymoPURETM Plasmid Miniprep Kit (Zymo Research)
Enzymes
Digestion enzymes
| Enzyme | Producer |
|---|---|
| AgeI-HF | NEB |
| BsaI | NEB |
| DpnI | NEB |
| DpnI | Jena Bioscience |
| EciI | NEB |
| EcoRV | Thermo Fisher Scientific |
| MnlI | NEB |
| NotI | Jena Bioscience |
| PstI | NEB |
| PstI | Thermo Fisher Scientific |
| PstI-HF | NEB |
| SapI | NEB |
| SpeI | NEB |
| XbaI | NEB |
| XbaI | Jena Bioscience |
Polymerases
| Enzyme | Producer |
|---|---|
| Go Taq DNA polymerase | Promega |
| Klenow Fragment | Thermo Fisher Scientific |
| Phusion High-Fidelity DNA polymerase | NEB |
| Q5 High-Fidelity DNA polymerase | NEB |
| Titanium Taq | Clonetech |
| Allin HiFi DNA polymerase | highQu |
| innuDRY DNA polymerase | Analytik Jena |
| BioMaster-HS Taq | Biolabmix |
| FirePol DNA polymerase | Solis Biodyne |
| Phusion High-Fidelity DNA polymerase | NEB |
Other Enzymes
| Enzyme | Producer |
|---|---|
| Shrimp Alkaline Phosphatase | Jena Bioscience |
| T4 DNA Ligase | NEB |
| T4 DNA Ligase | Thermo Fisher Scientific |
| T4 Polynucleotide Kinase | NEB |
