-
...se the yields of anthocyanin when paired with the genes in our anthocyanin construct. We have added the sequence to the registry for use in our composite part <
<h2><b> Melanin Construct</b></h2>
10 KB (1,730 words) - 17:37, 1 November 2017
-
...engineer <i>E. coli</i> to synthesize PHB. We designed a pET29b(+)-phaC1J4 construct to allow our engineered <i>E. coli </i> to employ the beta-oxidation pathwa
...nfirm the ability of <i>E. coli</i> transformed with our pET29B(+)-phaC1J4 construct to produce PHB, we grew O/N cultures for 24 hours and induced them with 0.1
34 KB (5,337 words) - 04:28, 16 December 2017
-
...sequence is identified as integral in determining whether the single chain construct has activity.</h4><br>
...ney_Australia/Patent_Law">patents</a>. With this in mind - we designed our construct to also have 12 amino acids.<br>
66 KB (8,543 words) - 03:14, 2 November 2017
-
...ttps://static.igem.org/mediawiki/2017/d/d1/T--Bielefeld-CeBiTec--composite-construct.png">
...fot the mRFP transcript is downstream the CDS of the gene of interest. In construct 2 the CDS of the mRFP is downstream a T3 promoter and the CDS coding for th
27 KB (4,095 words) - 01:18, 2 November 2017
-
...titutive promoter psaD (and its respective terminator) The detail of these
construct are shown as follows in the next image:</p>
<p id="FigureText">Fig. 1: Scheme that shows the fusion protein
construct to make the first transformation of <i>C. reinhardtii</i>.</p>
8 KB (1,220 words) - 00:35, 30 October 2017
-
In order for our construct to work, it had to be transfected into HEK2935, a mammalian cell line. RNA
We transfected our construct (BBa_K2340000) with a guide RNA plasmid (BBa_K2340001 - BBa_K2340011) (1:2
21 KB (2,470 words) - 04:21, 16 December 2017
-
...e our chances of successful assembly. We did succeed in inserting the melA construct into pJET (Figure 1).
...l of melA_pJET digested with EcoRI and PstI. Plasmids 1 and 3 had the melA construct successfully insert into pJET without disruption of the cut sites.</i></p>
8 KB (1,262 words) - 13:54, 1 November 2017
-
In addition, the tested construct was approximately 1500 bp long, which is also quite far from RPA's optimum
construct of 200 bp, one larger in size and less intense and one smaller in size and
11 KB (1,840 words) - 21:57, 31 October 2017
-
...titutive promoter psaD (and its respective terminator) The detail of these construct are shown as follows in the next image:</p>
<cite>Fig. 1: Scheme that shows the fusion protein construct to make the first transformation of C. reinhardtii.</cite>
9 KB (1,363 words) - 03:56, 2 November 2017
-
...l peptide in form of a fusion protein accompanied with His6x-Smt3 tag. The construct can be purified with commercially available and widely used affinity chroma
The construct can be purified with commercially available and widely used affinity chroma
10 KB (1,538 words) - 20:53, 15 December 2017
-
<p class="PP">This year, we aim to construct a diseases-monitoring system for automatic agriculture (AA) in which biolog
...ce<sup>[2]</sup>. What’s more, DAPG can also serve as signal molecule to construct a transcriptional switch that is similar to Tet on/off system<sup>[3]</sup>
31 KB (3,735 words) - 15:38, 1 November 2017
-
...;\n }\n if (arguments.length < 1) {\n throw new TypeError(\"Failed to construct 'SockJS: 1 argument required, but only 0 present\");\n }\n EventTarget.ca
...the same order.\n // 5. Return the result of calling the [[Construct]] internal\n // method of target providing args as the arg
932 KB (107,677 words) - 07:59, 24 October 2017
-
...;\n }\n if (arguments.length < 1) {\n throw new TypeError(\"Failed to construct 'SockJS: 1 argument required, but only 0 present\");\n }\n EventTarget.ca
...the same order.\n // 5. Return the result of calling the [[Construct]] internal\n // method of target providing args as the arg
932 KB (107,677 words) - 08:00, 24 October 2017
-
...and plated. Preliminary test of RFP expression with BBa_K1966004 (2016 Pu construct)
- Colony PCR on xylB construct – success (right)
66 KB (8,136 words) - 00:10, 2 November 2017
-
...aled for both species. Only the <i>gambiense</i> gene was used for further construct building. It was gel purified and digested with both KpnI and SacI. The bac
The construct building was repeated, since it failed in week 3. First, the ligation was r
8 KB (1,182 words) - 12:59, 31 October 2017
-
<p><b>Superior experiment:</b> Gaining more of the construct pSB1C3-AraC-chiA</p>
<li style="font-size: 15px;"> The mutation still remains in the construct</li>
96 KB (13,160 words) - 21:18, 1 November 2017
-
* IPTG Induction of BL21 promoter construct
* Transformation of BL21 with pick 4 of promoter B1C3 construct
10 KB (1,332 words) - 17:46, 31 October 2017
-
...P fluorescence obtained from BL21(DE3) with the pETDuet-GST-f-p+pET28a-GFP construct cultured in LB medium (10X40).</span>
...fluorescence obtained from BL21(DE3) with the pETDuet-GST-f-p+pET28a-EGFP construct cultured in MOPS medium (10X40).</span>
26 KB (3,823 words) - 18:49, 1 November 2017
-
...ts were run on a gel and gel purified in order to isolate the promoter-RFP construct. These constructs were then ligated using T4 ligase into pCAMBIA-MCS which
..., however there were colonies on the LB+Kan plates of <i>virB1</i>-pCAMBIA construct for both plasmid backbones, BBa_K1357010 and BBa_J04650.
19 KB (2,909 words) - 03:17, 2 November 2017
-
...ing. The construct was tested in HEK293FT cells, targeting five genes. The construct was transfected concurrently with a GFP donor.</p>
...ge of the knock-in event for GAPDH gene. The data were normalized to empty construct. Error bar is +- s.d.
20 KB (3,198 words) - 03:53, 2 November 2017
