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<td>Simple, easiest to construct</td>
...espective promoter (PphlF), and a GFP reporter (BBa_K2477008). We grew our construct in different molarities of fusaric acid (0µM, 100µM, and 500 
232 KB (33,473 words) - 02:45, 2 November 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,114 words) - 00:16, 2 November 2017
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construct.<br><br>
<li>Construct half of the flip-flop structure and express it in <i>E. coli.</i></li>
170 KB (16,709 words) - 06:32, 15 December 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,037 words) - 23:41, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
159 KB (17,948 words) - 20:12, 1 November 2017
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<h2 class="textheading1" style="float:center"><u> Full Construct Testing</u> </h2>
...of GFP, the data seemed to be a bit problematic. We took a few of our full construct colonies as well as a few wild type Kanamycin resistant colonies and we ino
12 KB (1,480 words) - 02:11, 2 November 2017
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...This figure shows restriction enzyme check electrophoresis result of NrtA construct.
<h4>Endolysin Construct</h4>
29 KB (4,011 words) - 19:07, 1 November 2017
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...licated many times and matured, this allows us to have more cells with the
construct that can produce more pigment faster.
...wasted on transcribing an overly-long mRNA transcript. The melA expression
construct is the improved part that we were able to characterize the most (see <a hre
12 KB (1,924 words) - 17:29, 1 November 2017
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<h3>Sensing Module Construct</h3>
...T_sensing1.png" alt="Sensing Module Construct" />Diagram 1. Sensing Module Construct</a>
23 KB (2,839 words) - 17:07, 21 November 2017
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...luorescence was measured 20 minutes after this pH shift. The asr-mCardinal construct was inducible by change from pH 7.0 to pH 5.0 and lower but no fluorescence
...s in LPM with pH 7 overnight. For expression control of our alx-mNeonGreen construct, we inoculated 20 ml LPM with pH 7.0, 8.0 and 8.5 to an OD<sub>600</sub> of
7 KB (1,003 words) - 03:29, 2 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,127 words) - 22:23, 1 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,092 words) - 09:44, 23 November 2017
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...se). Here we performed saturation mutagenesis on the <i> araC </i> gene to construct AraC mutant library with four randomized ligand-binding pocket amino acid r
...sors and may not show sufficient specificity to xylulose. Hence, we aim to construct an alternative regulatory system which exploits the L-arabinose operon.
39 KB (6,105 words) - 16:49, 17 November 2017
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...2 regulated systems. Herein we will refer to each promoter + split protein construct as a “module”, i.e. a full AND-gate will require the presence and activ
...er for split T7 RNAP function. We utilised a preexisting T7 promoter (pT7) construct from the BioBrick registry, [http://parts.igem.org/Part:BBa_K1321338 K13213
33 KB (5,136 words) - 02:41, 2 November 2017
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<h3>New plan for assembling the cas13 construct</h3>
<h4>New plan for assembling the cas13a construct: Plan A and Plan B</h4>
656 KB (66,631 words) - 09:22, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
160 KB (18,135 words) - 23:14, 1 November 2017
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...ed AcrIIA4 from G-block and amplified pSB1C3 backbone from LacILOV-mCherry Construct in pSB1C3</td>
...ILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
7 KB (797 words) - 19:53, 14 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
59 KB (9,038 words) - 03:50, 2 November 2017
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...ngs were obtained after 24 hours of incubation. This temperature sensitive
construct was working as expected in 10β cells, elucidating higher GFP expression at
<p id="imgdescription">Figure 2: BBa_K2447014
construct which was characterised in 10β cells (incubated overnight). At higher temp
20 KB (2,876 words) - 13:25, 1 November 2017
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...work in a better understood organism and led to higher copy numbers of our
construct. </p>
<p>Our final
construct, pGUIDE, consisted of Chloramphenicol and Kanamycin resistance genes, the C
29 KB (4,511 words) - 03:55, 2 November 2017
