Team:WashU StLouis/Notebook
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Micah, Maddie, Alex
Prepared LB Media
Re-suspended DNA (
D. Radiodurans
Uracil DNA Glycosylate 2) from iGEM kit
Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells
Prepared LB Agar
Prepared overnight culture to make competent cells (MHD42)
Micah, Maddie
Prepared MHD42 competent cells
Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
Transformation was a success with 100 colonies
Plate was stored in 4 deg. Celsius for future use
Prepared TSS Buffer
No Work!
No Work!
Micah, Maddie, Alex, Mark, Collin
Prepared Chloramphenicol (CM) plates
Prepared Ampicillin solution to be used for plates in the future
Made more LB Agar
