Team:TP-CC San Diego/InterLab

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Interlab

Introduction

This year’s interlab study is intended to answer the main question of the relativity of fluorescence when measured at different parts of the world. To make this data more reliable, iGem has asked to test some RBS devices to make gene expression reliable and precise. For this year’s interlab study, TP-CC San Diego has decided to do the plate reader protocol along with transforming the plasmids from Kit 7.

Method

OD​600​ Reference point

Procedure:

1. Add 100 µl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette)
2. Add 100 µl of H2O into wells A2, B2, C2, D2 (or 1 mL H2O into cuvette)

Data

uM Fluorescein

Fluorescein Standard Curve

Fluorescein Standard Curve (log scale)

uM Fluorescein/a.u.

Raw Plate Readings

Fluorescence Raw Readings:

Abs600 Raw Readings:

Results

Raw Abs600

Raw Fluorescence

OD - Background

Fluorescence - Background

uM Fluorescein / OD600

Summary Statistics