Team:Gaston Day School/Experiment

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Experiments and Protocol

Labels

For MCF tubes:

  1. P: Plasmid Prep
  2. D: Digest
  3. T: Transformation
  4. L: Ligation

Transformation

  1. x = the number of transformations you are doing
  2. Place the SOC medium (from the fridge) in the incubator
  3. Also place X plates in the incubator (With the appropriate antibiotic resistance, Chl for complete, Amp for parts)
  4. Make an ice-water bath
  5. Obtain X MCF tubes and a float
  6. Add 50 ul of competent cells
  7. Add 2ul of the DNA
  8. Place it in the ice-water bath for 2 mins
  9. While waiting, turn on the sink's hot water, obtain a beaker, add hot water, and using a thermometer, adjust water to 42o C (using the hot plate if needed)
  10. Place tubes in the incubator for 30 seconds
  11. Another 2 mins on ice
  12. Add 1 ml of warm SOC medium
  13. Place each tube in the incubator (37o C) for 1 hour
  14. Plating

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056