Team:Aalto-Helsinki/Parts
Basic Parts
This page lists all the basic parts that we have made and used during our project.
1] BBa_K2342000
DCD-1L, Dermcidin derived peptide
The part codes for the expression of dermcidin derived DCD1L peptide. Dermcidin is a recently discovered
antimicrobial peptide (AMP) found in primates with no homology to other know AMPs. It is expressed in
a constitutive manner in eccrine sweat glands and secreted to epidermal surface as a part of first line
of defense. Mature dermcidin precursor is 110 amino acid long, including signal peptide. Once antimicrobial
peptide precursor is secreted with sweat to epidermal surface, 19 amino acid long signal peptide is cleaved,
and it goes under further proteolytic processing leading to several dermcidin derived peptides such as
DCD1 and DCD1L. DCD1L is one of the most abundant form of dermcidin derived peptide. DCD1L is a 48 amino
acid long anionic peptide active against wide spectrum of bacteria including
Staphylococcus aureus,
Escherichia coli, and
Propionibacterium acnes.
For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008.
2] BBa_K2342001
SUMO fusion tag, Smt3
Employing of Smt3 tag is beneficial in several aspects in expression of recombinant proteins. Ulp1 is
a robust enzyme that specifically facilitates cleavage of Smt3, leaving protein of interest unbound to
tag. SUMO tag is known for its effect on solubility and preventing inclusion bodies of fusion peptide,
significantly easing purification step.
For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008, BBa_K2342009.
3] BBa_K2342002
CBM, Cellulose Binding Domain
This part encodes for Cellulose binding domain (CBM3) from
Clostridium thermocellum. This part could be used with any other construct to bind to cellulose.
For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008.
4] BBa_K2342003
6x Histidine tag
6x Histidine tag in is commonly used for purification of fusion proteins with Immobilized Metal Ion Affinity
Chromatography (IMAC) columns designed for histidine tagged proteins.
For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008, BBa_K2342009.
5] BBa_K2342004
10 amino acid linker, 5X GS
10 amino acid long linker contains glycine and serine repeats.
For more details on the production and usage of this part please check the composite part page: BBa_K2342007, BBa_K2342008.
6] BBa_K2342005
22 amino acid linker
22 amino acid long linker contains serine, alanine and glycine repeats.
For more details on the production and usage of this part please check the composite part page: BBa_K2342007, BBa_K2342008.
