Team:Aalto-Helsinki/Protocols
Protocols
Preparation of LB plates and LB broth preparation
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PCR amplification, Preparation of an agarose gel (1%) & electrophoresis, Sample preparation for Gel run, PCR product clean-up, DNA extraction from an agarose gel
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Restriction enzyme digestion and DNA ligation
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E. coli heat-shock competent cells preparation and E. coli heat-shock transformation;
Plasmid isolation, Check-up digestion of the cloned plasmids
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Small-scale protein expression, Protein purification with Qiagen Ni-NTA spin columns
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Larger scale protein expression (500 ml Batch), Cell lysis with AVESTIN EmulsiFlex-C3, Protein purification with ÄKTA and Buffer exchange and concentration of protein samples
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Digestion of Smt3-containing recombinant proteins with Ulp1; Preparation of an SDS-PAGE gel, electrophoresis and Sample processing for SDS-PAGE
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MALDI-TOF Mass Spectrometry, Dialysis, ZipTip and Sample preparation
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Antimicrobial assay, Standard curve for antimicrobial assay and Activity testing
Cellulose nanofiber (CNF) binding assay
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