Alkane Biosensor
——NWU-CHINA
Introduction
![](https://static.igem.org/mediawiki/2017/3/3c/NWU-Green.png)
We separated oil degradation bacteria DN1 from areas contaminated by oil. As oil degradation is an alternative metabolic pathway in bacteria, there is a manipulator for oil degradation gene, such as GntR. It codes GntR protein, which can bind with promoter on upstream of alkB2.
![](https://static.igem.org/mediawiki/2017/f/f5/NWU-Pink.png)
When alkane exists, GntR will unbind with the promoter, and the RFP gene we added on downstream will express to show a signal. When alkane exists, GntR will unbind with the promoter, and the RFP gene we added on downstream will express to show a signal. We used P.a DN1 and DH5α as our chassis to explore which bacteria will be a better chassis for our device.
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