Team:ETH Zurich/Composite Part

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Composite Parts

Here you can read details about our Composite Parts. To get an overview of all parts we created, visit Parts.

Overview

All parts were codon-optimized for expression in E. coli Nissle with Geneious and modified to remove forbidden restriction sites.

Part	Description	BioBrick
pConstRBSeng_TlpA	Best Composite Part: Constitutively expressed temperature-dependent transcriptional repressor of pTlpa with engineered RBS	BBa_K2500008

Design

BBa_K2500008: p_Const_RBS_eng_TlpA

Design notes: A RBS library was created using the Red Libs algorithm to find variants translating more TlpA RNA in order to create a thermoswitch tight enough to repress the premature induction of cell lysis by protein E.

Organism: Salmonella typhimurium

Based on: BBa_J23104 (promoter) and Piraner, Dan I., et al. Nature Chemical Biology 13.1 (2017): 75-80. (TlpA)

Source: FIXME RedLibs + gBlock

Registry: BBa_K2500008

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