Team:TecCEM/Demonstrate
Demonstrate
Math modeling with our results
We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The d and θ were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.
The following table shows the values for each point using the equation.
| X | Xm | T-Xm |
|---|---|---|
| 0 | 1 | 1 |
| 20 | 0.9 | 0.97992 |
| 40 | 0.6 | 0.632184 |
| 60 | 0.57 | 0.285714 |
| 100 | 0.25 | 0.177328 |
The following graph shows that the mRNA degradation does exist, but it doesn’t have the same behavior it is not as reduced as the mode, this means that at a bigger concentration the greater the degradation induced by the siRNA.
siRNA confirmation
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