Team:Fudan/Basic Part
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This part is a surface-expressed EGFP which can be used to set up heterogeneous antigen density expression cell model. Our team is very excited to be the first to bring the idea of antigen density heterogeneity to the iGEM competition, and propose a tri-response immunotherapy, SwordS.
Surface-expressed EGFP (surEGFP) is built by fusing CD8α signal peptid and EGFP to the transmembrane region of CD86 (NM_175862) from N terminal to C terminal. CD8α peptide guides surEGFP to be located to cell membrane. transmembrane region of CD86 supports surEGFP to fix on membrane.
Integrated information about surEGFP posted on Demonstrate page: Antigen density heterogeneity cell model. Or you can alternatively check the (BBa_K2446051) on the Registry of Standard Biological Parts website.
We listed other basic part we constructed this year here.
| 2_ZF42-10/43-8 binding site | (BBa_K2446001) |
| 2_ZF54-8/43-8 binding site | (BBa_K2446002) |
| 2_ZFHD1 binding site | (BBa_K2446003) |
| 2_PIP binding site | (BBa_K2446004) |
| 4_ZF_42-10 binding site | (BBa_K2446005) |
| 4_ZF_43-8 binding site | (BBa_K2446006) |
| 4_ZF_54-8 binding site | (BBa_K2446007) |
| 4_ZF_21-16 binding site | (BBa_K2446008) |
| 4_ZF54-8/43-8 binding site | (BBa_K2446009) |
| 4_ZFHD1 binding site | (BBa_K2446010) |
| 4_PIP binding site | (BBa_K2446011) |
| 8_ZF_42-10 binding site | (BBa_K2446012) |
| 8_ZF_43-8 binding site | (BBa_K2446013) |
| 8_ZF_54-8 binding site | (BBa_K2446014) |
| 8_ZF_21-16 binding site | (BBa_K2446015) |
| 8_ZF_54-8/43-8 binding site | (BBa_K2446016) |
| 8_PIP binding site | (BBa_K2446017) |
| ZF_GAl4_KRAB | (BBa_K2446037) |
| ZF_PIP_KRAB | (BBa_K2446038) |
| ZF_21-16KRAB | (BBa_K2446039) |
| ZF_42-10_KRAB | (BBa_K2446040) |
| ZF_43-8_KRAB | (BBa_K2446041) |
| ZF_54-8_KRAB | (BBa_K2446042) |
| ZFHD1_KRAB | (BBa_K2446043) |
