Team:RPI Troy NY/Results

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Results

After culturing our control yeast strain, one with a full MFE-2 gene knockout and one wild-type, it appears there was not enough separation in the flasks to indicate enough sophorolipid yield. Due to unforeseen issues with primer design as well as fusion PCR delayed the microbial genetics committee a few weeks. A new protocol has been created by Dr. Gross and this will be implemented throughout the next 6 days. Final results will be presented by the team at the Giant Jamboree.

Preliminary Results

Below are previous Sophorolipid LCMS Data collected. This data will no longer be used. Final data to be presented at the Jamboree.


Below are the final data as presented at the Giant Jamboree. Our data demonstrate that selective modification of the Starmerella bombicola (yeast) MFE2 gene improves the efficiency of sophorolipid production.