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Results
After culturing our control yeast strain, one with a full MFE-2 gene knockout and one wild-type, it appeared there was not enough separation in the flasks to indicate enough sophorolipid yield. Unforeseen issues with primer design as well as fusion PCR delayed the microbial genetics committee by a few weeks. A new protocol was created by Dr. Gross and was implemented from November 3 through November 9. Final results were presented by the team at the Giant Jamboree.