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...aldehyde. The aldehyde was then condensed with <i>N</i>-methylcysteine to yield the pyochelin. Due to poor commercial availability, <i>N</i>-methylcysteine ...g of a pale-yellow, foliaceous solid were obtained. This corresponds to a yield of 68 %.</u><br>

...ow that the production inside a peroxisome can greatly increase nootkatone yield. Afterwards we discuss possible ways to overcome the shortage of the cellul plt.ylabel(r&quot;Yield in $\frac{mol}{cell}$&quot;)

<p>We also tried to measure the yield of γ－aminobutyric acid (GABA) by using amino acid analyzer (AAA). Firstl ...supernatant was processed and tested. Fig. 4 shows the result of the GABA yield by <i>E. coli</i> Nissle with the constructed plasmid. However, there was n

.... Ignoring the finite resources of the cell may skew our prediction of the yield of our expression systems. See below for details on the whole cell model we ...l model from [1], and performed in silico experiments, comparing predicted yield and parameter scanning to discover any insights into how to optimise recomb

...mentation process, problems like low efficiency of gene expression and low yield always occur. The formula of culture media and the condition of culturing i ...25 ,the yield of BC the most optimal.Meanwhile, when pH=5.25 and 4.75, the yield of BC are higher than the control group by 11% and 7%.</p>

...m.com/eu/products/escherichia-coli-media/media-for-optimal-cell-growth-and-yield-of-e-coli-cultures.html <div class = "title"><p>Yield: </p></div>

the specific rate of sugar production and the yield of biomass on sugar at each position in the bulb.</p> <br>Y_phm^c = Maximal yield of sugar on photons</p>

acid yield by HPLC after whole cell catalysis. Because there are a large number yield multiple is a new indicator, the result is as follows:

...ub(8|16)|[su]xt(a?h|a?b(16)?)|srs([id][ab])?|swpb?|swi|smi|tst|teq|wfe|wfi|yield)(eq|ne|cs|cc|mi|pl|vs|vc|hi|ls|ge|lt|gt|le|al|hs|lo)?[sptrx]?",end:"\\s"},e ...ng from get group into join let orderby partial select set value var where yield",n=e.IDENT_RE+"(<"+e.IDENT_RE+">)?";return{aliases:["csharp"],keywords:t,il

...em>^[1], which allows us to predict optimal steady-state biomass yield, which is tightly correlated with cell growth rate and is the most likely o ...itially contained 1550 reactions and 1556 metabolites. The optimal biomass yield was found to be \( 224.3248 (\text{g dry weight}\cdot\text{h})^{-1} \). Nex

...iments that would screen the following factors for their effect on protein yield: <br/><ul> ...ual factors and the two-way interactions between the factors that affected yield (see <b>Figure 8<b>). </p>

...<li>Optimized co-culture media can increase the yield of BC by16.7%</li> ...ering,and the yield of BC's is also low. According to our finding that the yield of BC was higher than that of <i>G.xylinus</i> cultured individually when c

...in BC synthesis process. Through these two methods we hope to achieve high yield of BC . Therefore, we designed the following experiments:</p> ...fer molecule of bacteria.the goal is to implement both to realize the high yield of BC.</p>

...<li>Optimized co-culture media can increase the yield of BC by16.7%</li> ...ering,and the yield of BC's is also low. According to our finding that the yield of BC was higher than that of <i>G.xylinus</i> cultured individually when c

...roduction of alcohols, large-scale bacterial fermentation is often used to yield more product. However, when the alcohol level in the growth medium nears th ...creasingly important. However, mining, refining, and recycling of them may yield hazard by-product if not properly managed. Therefore, we have attempted to

<li>Measured the TetR gene concentration. Low yield of 4.3ng/microL obtained. Sample not workable and quite contaminated. Gel e ...el and excised. Yield of DNA and purity measured using the nanodrop. - Low yield of 6.6ng/microL.</p>

<h3> How to Improve the Yield of Bacterial Cellulose(BC)?</h3> ...ution as buffer. After measure the parameters of pH, residue sugar and the yield of BC after 10days of fermentation. We confirm the optimal pH for the ferme

...pective of the growth rate, as long as cells are illuminated, the fumarate yield on biomass is constant. This result also support theoretical predictions, w ...e day is growth coupled. We calculated the fumarate yield during the day, yield

...isomes when growing under glucose-rich conditions, biosynthesis and target yield can be increased by altering peroxisome size and number. A short introducti ...showing both number and size increase, also leading to a higher metabolic yield. However, a Pex31,32 knockout has been proven to attribute to a change in t

<p>where \(Y\) is known as the <i>yield constant</i></p><br> & = \textrm{input} - \textrm{output} - \frac{\textrm{growth}}{\textrm{yield constant}}

...ons into the natural stress-resistant peroxisomes and thereby increase the yield and efficiency of rare biomolecule production <em>in vivo</em>. ...ytosolic enzymes with membrane bound ones to see if there is any change in yield.</p>

catalyst MoBiFe, the yield of acrolein can reach more than 90%. The reaction ...Mo, Bi, Fe, Co, K and O. The yield of acrylic acid can reach 97.5%. The yield

For the HisTrap column, we observe a purification yield equal to 72.1 %. For the Gluthation Agarose Resin we observe a purification yield equal to 32%.

...ns into the natural stress-resistant peroxisomes and thereby increases the yield and efficiency of rare biomolecule production <em>in vivo</em>. ...ytosolic enzymes with membrane bound ones to see if there is any change in yield.</p>

...tion volume between 20-50&nbsp;&mu;L does not significantly reduce the DNA yield. However, elution volumes less than 10&nbsp;&mu;L are not recommended.</sma ...imer-dimers, purification without isopropanol is recommended. However, the yield of the target DNA fragment will be lower.

// If there's more to do, yield // The conditional operator is the lowest precedence operator (except yield,

...cal pesticides. The yield of herbicides decreased 10.87% compared with the yield in April 2016. From January to April 2017, Chinese chemical pesticide produ <th>Yield in April/ton</th>

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...Part</a>) but also produces Glucose, Mannose and other sugars reducing the yield of production. ...ed to generate a bank of mutant epimerases to find an enzyme with a better yield. In industries, biologists and PI informed us about the difficulties in enz

<li>Measured the TetR gene concentration. Low yield of 4.3ng/microL obtained. Sample not workable and quite contaminated. Gel e ...el and excised. Yield of DNA and purity measured using the nanodrop. - Low yield of 6.6ng/microL.</p>

..., 2015). One study looking at PHA production from food waste estimated the yield of 0.05 g of PHA per g of COD applied (Rhu, Lee, Kim & Choi, 2003). Another ...n be produced per year in Hong Kong depending on COD content and using PHA yield of 0.11 kg of PHA per kg of COD. PHB production from leachate was also cons

The qPCR did not yield an result. In the following, qPCR was not referred to as a option for phage ...inal domain of the T7 RNAP. Even the sequencing of over 10 plaques did not yield any mutations in the essential domain.

<br> In order to determine the yield of the target product, we chose to observe the efficiency of the DHAP yield estimation system in view of the lack of basic ceaS2 enzyme data.

...ch is important with respect to the project as it predicts the theoretical yield of desired product (tagatose) in our system, is visualized through an enzym ...ects the efficiency, while that of the substrate will increase the overall yield</b>.</p>

$\ce{R}$ is first transcribed to yield the mRNA $\ce{M_R}$. The transcript is then translated into the protein $R$ yield the corresponding repressed operators $\ce{R_2O_N}$ and $\ce{R_2O_E}$. This

<p><strong>Cycle number</strong>: Generally, 25–35 cycles yield sufficient product. For genomic amplicons, 30-35 cycles are recommended.</p ...Buffer at a higher temperature (37°C to 50°C) may slightly increase the yield, especially of large (> 10,000 bp) DNA plasmids.</li>

...e cell wall is essential for efficient lysis. Longer incubation time might yield better results.</i></li> ...oom temperature prior to centrifugation will give a modest increase in DNA yield per elution.</li>

<h2>Optimise recombinant Insulin/Winsulin Yield</h2> <h4>Increasing yield would be the most important aspect of improving our product to make large s

...nt energy market due to the absence of polluting emissions and high energy yield. As hydrogen fuel cells generate energy via the redox reaction of hydrogen ...ith the <i>C. reinhardtii</i> FNR, we aim to get closer to the theoretical yield for hydrogen gas production from glucose, which is twelve molecules of hydr

...om the cytosol into the peroxisome and by this increases the productivity, yield and efficiency of the pathway’s end product. We chose nootkatone and viol ...s would be to develop a more efficient system. An easy way to increase the yield of compounds in the supernatant is to manipulate the <a href="https://2017.

...atial organization of enzymes belonging to a certain metabolic pathway the yield of this pathway may be increased. This was for example investigated for the To investigate whether applying metabolic channeling enhances the yield of the indole-3-acetic acid pathway we devised two different approaches nex

SDS 10⁹ seems to have lower yield than NaOH -> maybe more SDS? (10⁸ has no problems...) Terrible yield, repeat again to be sure

SDS 10⁹ seems to have lower yield than NaOH -> maybe more SDS? (10⁸ has no problems...) Terrible yield, repeat again to be sure

<li> 10 nmol ligation because not 100% yield, therefore we need to make more than necessary.</li> ...r at least 20 minutes (can leave overnight at this temperature for greater yield)</li>

As shown in Fig.1, multiple synthesis pathways could easily be imagined to yield the desired compound. ...ed system. This compound is then hydrolysed by addition of aqueous acid to yield the formyl-derivative of the phenolic compound. In the same step the urotro

...<li>Superior broth has more nutrients than LB and therefore has a higher yield</li> ...<li>Note: Using more elution buffer will increase overall yield of DNA but will result in final solution of eluted DNA being less concentra

<p> Both gel extracted samples barely have a yield and the product is the wrong size.</p> ...amplification of the insert (on the Gibson assembly mix) to produce a high yield of product. If this works, we could then ligate the product to the pGEM clo

...ls per milliliter for an overnight culture [3], this would correspond to a yield of $0.432$ ...ed <i>in vitro</i> under equimolar amounts of GFP1-9 and GFP10-SR-GFP11 to yield a time-intensity

alt='The relation between photosynthetic rate and total yield' <th>the instantaneous yield coefficient of product formation due to cell growth</th>

...A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes. </p><Br> ...ng the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimiz

...its wider application. Therefore, our project is dedicated to increase the yield of bacterial cellulose ...ay and external fermentation conditions of G.xylinus hoping to improve its yield of BC

...ssful use of dissolved air flotation (DAF) to separate PHB with an overall yield of 86% in lab-scale experiments (van Hee, Elumbaring <i>et al.</i>, 2006). .... The process also has not been used in scaled-up applications yet and PHB yield is comparatively low when compared to solvent extraction. We would also nee

...esired product, but also to optimize growth conditions to maximize product yield within a given timeframe. Take a look at our <a href="https://2017.igem.org

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...and number of peroxisomes while at the same time lead to higher metabolic yield. For our purpose he recommended working with a <a href="https://2017.igem.o ...ly the intermediates are stored close to the membrane. To achieve a higher yield it was suggested to fuse the relevant enzymes valencene synthase (ValS), P4

...purification concept in which the surface of the column is crucial for the yield instead of the volume compared to conventional purification techniques. ...ely, this could lead to a loss of the target protein and thus decrease the yield. The major challenge concerning the flow-bundle-like column is the irradiat

Cells were fractionated to yield cytoplasmic by the cell lysis solution ,and periplasmic samples by the Argi 3) low yield of recombinant proteins due to weak induction efficiencies (much less than

...>Figure 2.</b> The interaction profile of the input factors, maximised for yield. OD600 at induction consistently correlated with higher yields. The profile ...s within each variable towards the optimum, thus shifting the range of our yield to a higher level.

...uring health and the chemical balance, hydroponic growers can improve crop yield and thus their margins. OxyPonics provides comprehensive, remote, real-time ...this issue - and OxyPonics does just that by boosting crop performance and yield while keeping capital costs to a minimum.

...luding high costs from HVAC systems and low margins due to disease and low yield. We realized that our system needed to be affordable as well as effective i ...ld help the hydroponic industry grow. Since OxyPonics would increase plant yield, we believe there is tangible need for our product. Additionally, OyxPonics

...>An important part in engineering is the simulation of systems in order to yield an insight of the expected behaviours before actual ...for salicylate and estrogen respectively. Concentrations lower than those yield either no or a weak response relative to the maximal

...mentation process, problems like low efficiency of gene expression and low yield always occur. The formula of culture media and the condition of culturing i ...25 ,the yield of BC the most optimal.Meanwhile, when pH=5.25 and 4.75, the yield of BC are higher than the control group by 11% and 7%.</p>

<td>Y_E/g</td> <td><i>E.coli</i>-biomass yield</td> <td>287.54</td> <td>/particle</td> <td>Estimated from experimental dat ...<td>Y_S/g</td> <td><i>S.cerevisiae</i>-biomass yield</td> <td>20.01</td> <td>/particle</td> <td>Estimated from experimental data

<p>Bacterial yield</p> <p>Bacterial yield</p>

...ocess independently, it would be difficult to realistically project price, yield, and environmental impact of the latex manufacturing method; The environmen ...ormation for iGEM Project,"<ul><li> Visit facility and discuss production, yield, and development of PET industry</li><li> Discuss project with manager</ul>

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

...ifficult to clone the two gBlocks together, and whenever we succeeded, the yield was too low. We also found a few other mistakes in the design of the sequen ...which should have no colonies due to toxin expression, showed the highest yield. After receiving the sequencing results, we found mutations in some nucleot

...as successful whereas transformation using the cells from our lab didn’t yield good results.</li> <li class="points">Ligation didn’t yield positive result.</li>

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To increase the yield of the yield of

...uring health and the chemical balance, hydroponic growers can improve crop yield and thus their margins. OxyPonics provides comprehensive, remote, real-time ...this issue - and OxyPonics does just that by boosting crop performance and yield while keeping capital costs to a minimum.

...ol metabolic pathway in organisms and construct a cell factory with a high yield of acrylic acid. acid. However, the yield of the cell factory 1.0 is not high, only about 1mg / L.

...also tested two super-rich media (TB and SOB) in attempt to produce higher yield of bacteria in shorter time. To test the possible growth in minimal media w ...:3%"> To determine which media and temperature is optimal for the best DNA yield cultures in LB, SOB and TB in 37 °C and 42 °C. This experiment was not do

...re, we wanted to test the highest concentration of fructose and see if the yield could be enhanced. So, we used higher concentrations of fructose and we obs ...lass="text-left">Finally, we wanted to investigate if we could enhance the yield of the bioproduction by increasing the IPTG induction, thus increasing the

...to obtain a quantitative analysis of the GST and thus a final purification yield. From this enzymatic activity, a yield can be obtained. Yield = Final total activity / Initial total activity

...system provided that the cell functions normally - the optimal production yield.</p> ...ints that need to be considered at the same time. For the standard optimal yield simulation, a mathematical representation can be formulated in this way:</p

...reas the first plasmid came from a miniprep that is known to give a better yield.</p> ...tending the incubation time after buffer addition to 5 minutes can improve yield.</i></p>

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...ls because transformations performed with ligation products usually do not yield as many colonies due to the low DNA concentration in the ligation mixture. ...of each concentration if possible, so you can calculate an average colony yield.

...ep up my game. Performed miniprep on G-3 bacteria inoculation culture. DNA yield was only 2.1ng/uL. Fear not, tomorrow will be the day! Continued work on pi <b>Vi Ka:</b> Performed miniprep on G-3 and GFP, with Epoch kit. Achieved yield of 62.7 ng/uL for GF-3, and 144.7 ng/uL for GFP. Prepared digestion for G-3

...ly repress active domains of this enzyme with the intent of increasing the yield of sophorolipid product. By employing a repression technique rather than a

...expression of the biosynthetic pathway, thus maximizing enzyme and pigment yield. </p> <p>Yan, Y., Li, Z., and Koffas, M. A. (2008) High-yield anthocyanin biosynthesis in engineered Escherichia coli. Biotechnology and

...gh (85°C), so using different additives/temperature gradients to optimize yield</li> ...ne + UNS was actually amplified during PCR, and was not visible due to low yield.</li>

...gh genetic engineering could reduce production costs through improving the yield of useful chain lengths.</p>

larger volumes of EB will increase the overall yield of DNA, while using concentration is more important than yield, so go with the 15 µl amount.

<p>Bacterial yield, or bacterial cells per unit substrate</p> ...ed and infected bacteria, multiplied by the inverse value of the bacterial yield (<em>Y</em>), and concentration of inflow and outflow medium:</p>

...the soil and causes root systems to rot, which drastically decreases crop yield in fields nearby. With excessive oil accumulating in the soil, even crops g ...surfactin isoforms produced by different engineers with elevated producing yield, as shown in Fig. S7. Results indicated that overexpression of the three pu

<p><ul><li>Lower recorded yield than other methods</li> ...h secreted and intracellular PHB pellets were dried overnight and relative yield between the two fractions was compared. </p>

...many advantages with the occurrence of inclusion bodies. On one hand they yield a very high protein expression level, on the other, they are easy to isolat <td>Chambon, R., Pradeau, S., Fort, S., Cottaz, S., Armand, S. (2011) High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro sy

<p>Plasmid DNA extractions are performed using Hi Yield^® Plasmid MINI kit according to the <a href="http://www.suedlabo <p>PCR clean-ups and Gel extractions are performed using Hi Yield^® PCR Clean-up/ Gel Extractions Kit according to the <a href="ht

<p> This equation can be solved either analytically or numerically to yield solutions as follows.</p> ...al solution requires intelligent initial and boundary condition choices to yield reasonable results. Computational tools^[6] were used to solve th

...The baculovirus expression system is widely used in biotechnology for high yield recombinant protein expression by exploiting the life cycle of the virus in MAYV genomic RNA was isolated from a Trizol sample. After the integrity and yield of the MAYV RNA was checked, cDNA was generated using Superscript RTII acco

...advantages in our system. First, the protein production is faster, and its yield is higher due to its ability to auto replicate itself. The second one is re ...ral delivery arises as a novel tool due to its high protein expression and yield production. In the last years, it has been reported several virus-derived s

...of CCMB80 buffer needed to add to the resuspended cells to achieve a final yield of OD 600 1.0-1.5. ...://parts.igem.org/Part:BBa_K1321305">K1321305</a>) produced cellulose with yield treatment method was exposed to cell lysate containing phytochelatin-CBD (K

...of CCMB80 buffer needed to add to the resuspended cells to achieve a final yield of OD 600 1.0-1.5. ...://parts.igem.org/Part:BBa_K1321305">K1321305</a>) produced cellulose with yield treatment method was exposed to cell lysate containing phytochelatin-CBD (K

...effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we ...<br><br> In order to determine the effect of the new route on the yield of acrylic acid before the wet experiment

larger volumes of EB will increase the overall yield of DNA, while using concentration is more important than yield, so go with the 15 µl amount.

...herefore, we decided to change the order of our operon to maximize the PHB yield produced by this pathway.</p>