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Bielefeld-CeBiTec

/* START OVERIDE HQ CSS */

top_title {

display: none; }

sideMenu {

display: none; }

content {

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globalWrapper {

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HQ_page p {

font-size: 10pt; }

HQ_page table {

margin: 0px; margin: auto; }

ul { list-style-image: none; }

li { margin: 0px; margin-left: 25px; margin-bottom: 4px; }

a, a:link, a:visited, a:active { color: #9b4d75; text-decoration: none; }

a:hover { color: #cb7da5; text-decoration: none; }

.mw-content-ltr ul, .mw-content-rtl .mw-content-ltr ul { margin: 0px; }

groupparts {

margin: auto; }

/* END OVERIDE HQ CSS */

/* START OUR WIKI CSS */

table, th, td { border-collapse: collapse; border: 1px solid #dddddd; }

table { width: 96%; table-layout: fixed; margin: auto; margin-top: 15x; margin-bottom: 15px; }

table.parts { width: 62%; margin-bottom: 20px; }

th { background-color: #efefef; text-align: center; }

tr { background-color: #ffffff; height: 30px; }

td { background-color: transparent; padding: 10px; }

tr:hover { background-color: #efefef; }

,
before,

after {

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bodyContent a[href^="https://"], .link-https {

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margin: 0px; }

body { height: 100%; font-family: verdana; font-size: 12px; color: #000000; margin: 0px; }

/*styles bar of the navigation bar and fixes it to the top*/ header { background-color: #FFFFFF;

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top: 16px; display: table-cell; vertical-align: bottom; z-index: 10; }

footer { background-color: #333333; text-align: center; float: left; width: 100%; height: 80px; padding-top: 15px; box-shadow: 0px 0px 5px #000000; margin: 0px; }

navbar-left, #navbar-right {

box-shadow: 0px 0px 5px #000000; }

/*Aligns navbar items to the bar and removes bullet points*/ ul.navbar { list-style-type: none; margin: 0; padding: 0; overflow: hidden; background-color: #6b1d45; font-size: 14px; }

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ul.navbar.center { width: 6%; float: left; padding: 1%; padding-bottom: 5px; padding-top: 5px; box-shadow: 0px 0px 5px #000000; }

ul.navbar.center:hover { /*background-color: #7b2d55;*/ background-color: #62153d; }

/*changes the order of the items from vertical to horizontal*/ li.navbar, .sub-navbar-item { float: left; margin: 0px; }

.navbar.item-left { float: right; }

.navbar.item-right { float: left; }

/*styles the single items from text-links to nice buttons*/ li.navbar a, .sub-navbar-item a { display: block; color: white; text-align: center; padding: 0px 16px; text-decoration: none; }

/*changes the item color when the cursor is hovering over it, clicking it or the tab is active*/ li.navbar a:hover, li.navbar a:active, li.navbar.active,

li.navbar.highlighted {

background-color: #62153d; }

/*changes the item color when the cursor is hovering over it, clicking it or the tab is active*/ .sub-navbar-item a:hover, .sub-navbar-item a:active, .sub-navbar-item.active,

.sub-navbar-item.highlighted {

background-color: rgba(0,0,0, 0.35); }

.sub-navbar {

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   position: fixed;
   top: 6000px;

z-index: 9; }

.sub-navbar-list { list-style-type: none; margin: 0; padding: 0; overflow: hidden; background-color: rgba(0,0,0,0.7); top: 0; width: 100%; }

.sub-navbar-item { font-size: 13px; height: 26px; line-height: 26px; }

.sub-navbar-item.small { font-size: 12px; }

.anchor-jump{

 display: block;
 height: 60px; /*same height as header*/
 margin-top: -60px; /*same height as header*/
 visibility: hidden;

}

/* bevel size, left offset and color settings next 3 lines */ /*.bevel, .content { border-width: 30px } .bevel, .content { border-color: #eeeeee; border-style:solid; }

.contentbox .tr, .contentbox .tl, .contentbox .br, .contentbox .bl { height: 0px; width: 100%; } .contentbox .tr, .contentbox .tl { border-top: 0; } .contentbox .br, .contentbox .bl { border-bottom: 0; } .contentbox .tr, .contentbox .br { border-right-color: transparent; } .contentbox .tl, .contentbox .bl { border-left-color: transparent; } .no_bevel { height: 0px; width: 100%; border-bottom: 0; }*/

.container { width: 100%; margin-top: 16px; background-color: #FFFFFF; }

/*styles the background of the content boxes and orders them below each other*/ .contentbox { width: 100%;

   margin: auto;
   /*margin-bottom: 30px;*/

background: #f0f0f0; }

.contentbox:nth-child(even) { background-color: #ffffff; }

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@media screen and (max-width: 800px) {

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}*/

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.contentbox.team { width: 100%; background-color: #FF0000; padding-left: 0px; padding-right: 0px; display: flex; }

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/*surrounds the content by boxes with round edges and adds shadows*/ .content {

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overflow: auto; margin: auto; padding-bottom: 20px; }

.content.oligo-table{

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}

article, .article { text-align: justify;

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}

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overflow: auto; margin: auto; padding-bottom: 20px; display: none; }

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/*---*/ .labnote-date { padding: 1px; padding-left: 50px; padding-right: 50px; padding-top: 20px; padding-bottom: 10px; text-align: center; margin: auto; }

/*icons from font-awesome*/ .fa { color: #dddddd; text-align: center; }

/*color change for icons*/ .fa:hover { color: #ffffff; }

.figure, .sponsor-figure { display: block; margin: auto; margin-top: 20px; margin-bottom: 20px; }

.figure.large, .sponsor-figure.large { width: 92%; }

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/*Styles the figure's subtitle to a smaller font-size. */ .figure.subtitle, .figure.subtitle p, .sponsor-figure.subtitle, , .sponsor-figure.subtitle p { font-size: 10pt; text-align: justify; margin-top: -10px; margin-bottom: 20px; }

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/* */ .half.left { float: left; margin-right: 1%; }

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}

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transform: translate(-50%, -50%); overflow: auto; margin-top: 25px; }

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cover-img {

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cover-p {

text-align: justify; }

title {

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title img{

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}

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width: 100%; }

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color: #FFFFFF; font-size: 30px; width: 60%; margin: auto; }

a.no-link { color: #ffffff; }

footer-left {

width: 25%; float: left; }

footer-center {

width: 50%; float: left; }

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page-arrow-top {

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position: fixed; bottom: 20px;

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}

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p.table-headline { font-size: 12pt; }

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html, body {

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   overflow-x: hidden;

}

With their broad chemical and functional diversity ncAAs provide a variety of promising applications including proteinlabeling, photocaging, structure analysis and specific protein interactions. Therefore, the development of a new iGEM toolkit for the translational incorporation of non-canonical amino acids (ncAAs) in E. coli would be a great contribution to the iGEM community. The site-specific incorporation of ncAAs requires the directed evolution of tRNA and aminoacyl tRNA synthetase pairs, which in turn mediate the introduction of a ncAA for a certain codon. We are especially exploring the application of unnatural base pairs (UBPs) as an expansion of the genetic code. This approach promises an optimal orthogonality to the autologous translation apparatus and a high flexibility concerning the incorporation of multiple ncAAs. We will utilize several systems to achieve a high retention efficiency. As examples for the successful incorporation of non-canonical amino acids we will develop a rapid test for prions and a new chromatography method for a mild elution of proteins.

For more information visit our project poster on reasearch gate

Our wiki is at the moment under construction, but you can follow our social media to be up to date!

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You can also contact us per email for collaborations and more! :)

info[@]igem-bielefeld.de

Team:Bielefeld-CeBiTec

Bielefeld-CeBiTec