Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Methods"

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<h3> Protocols </h3>
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<ul>
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<!-- Ueberschriften -->
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<li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li>
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<h3> Protocols </h3>
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<li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--protocol_biotynilation.pdf" target="blank">Biotynilation of glass slides</a></li>
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<li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li>
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</article>
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<li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li>
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<li><a href="https://static.igem.org/mediawiki/2017/a/ab/T--Bielefeld-CeBiTec--protocol_electrotrafo.pdf" target="blank">Transformation via electroporation</a></li>
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<li><a href="" target="blank">Fast cell lysis buffer</a></li>
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<h3> Enzymes </h3>
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<li><a href="" target="blank">Lorem</a></li>
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<td>Enzyme</td>
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<td>Producer</td>
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<td><a href="#"><i>EcoR</i>I</a></td>
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<td>NEB</td>
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<li><a href="" target="blank">Lorem</a></li>
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<li><a href="" target="blank">Lorem</a></li>
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<td><a href="#"><i>Pst</i>I</a></td>
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<li><a href="" target="blank">Lorem</a></li>
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<td>NEB</td>
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<li><a href="" target="blank">Lorem</a></li>
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<li><a href="" target="blank">Lorem</a></li>
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<td><a href="#">T4 DNA Ligase</a></td>
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<td>NEB</td>
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</ul>
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<h3> Enzymes </h3>
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<h4> Digestion enzymes </h4>
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<thead>
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<th>Enzyme</th>
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<th>Producer</th>
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</thead>
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<tr>
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<td><a href="https://www.neb.com/products/r3552-agei-hf"><i>Age</i>I-HF</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0535-bsai"><i>Bsa</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0176-dpni"><i>Dpn</i>I</a></td>
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<td>NEB</td>
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</tr>
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<td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/d-enzymes/en-160-dpni"><i>Dpn</i>I</a></td>
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<td>Jena Bioscience</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0590-ecii"><i>Eci</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.thermofisher.com/order/catalog/product/ER0301"><i>Eco</i>RV</a></td>
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<td>Fermentas</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0163-mnli"><i>Mnl</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/n-enzymes/en-124-noti"><i>Not</i>I</a></td>
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<td>Jena Bioscience</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0140-psti"><i>Pst</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.thermofisher.com/order/catalog/product/ER0611"><i>Pst</i>I</a></td>
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<td>Thermo Fisher Scientific</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r3140-psti-hf"><i>Pst</i>I-HF</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0569-sapi"><i>Sap</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0133-spei"><i>Spe</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/r0145-xbai"><i>Xba</i>I</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/x-enzymes/en-143-xbai"><i>Xba</i>I</a></td>
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<td>Jena Bioscience</td>
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</tr>
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</table>
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<h4> Polymerases </h4>
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<thead>
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<tr>
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<th>Enzyme</th>
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<th>Producer</th>
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</thead>
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<tbody>
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<td><a href="https://www.promega.de/products/pcr/endpoint-pcr/gotaq-dna-polymerase/?catNum=M3001">Go Taq DNA polymerase</a></td>
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<td>Promega</td>
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</tr>
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<tr>
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<td><a href="https://www.thermofisher.com/order/catalog/product/EP0054">Klenow Fragment</a></td>
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<td>Thermo Fisher Scientific</td>
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</tr>
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<tr>
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<td><a href="https://www.promega.de/products/pcr/endpoint-pcr/gotaq-dna-polymerase/?catNum=M3001">Phusion High-Fidelity DNA polymerase </a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/m0491-q5-high-fidelity-dna-polymerase">Q5 High-Fidelity DNA polymerase </a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.jenabioscience.com/molecular-biology/standard-pcr/ready-to-use-mixes/pcr-108-red-load-taq-master">Taq Polymerase </a></td>
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<td>Jena Bioscience</td>
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</tr>
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</tbody>
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</table>
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<h4> Other Enzymes </h4>
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<tbody>
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<tr>
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<th>Enzyme</th>
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<th>Producer</th>
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</tr>
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<tr>
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<td><a href="https://www.jenabioscience.com/molecular-biology/modifying-enzymes/ligases-phosphatases-kinases/en-174-shrimp-alkaline-phosphatase-rsap">Shrimp Alkaline Phosphatase</a></td>
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<td>Jena Bioscience</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/m0202-t4-dna-ligase">T4 DNA Ligase</a></td>
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<td>NEB</td>
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</tr>
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<tr>
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<td><a href="https://www.thermofisher.com/order/catalog/product/15224017">T4 DNA Ligase</a></td>
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<td>Thermo Fisher Scientific</td>
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</tr>
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<tr>
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<td><a href="https://www.neb.com/products/m0201-t4-polynucleotide-kinase">T4 Polynucleotide Kinase</a></td>
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<td>NEB</td>
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</tr>
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</tbody>
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</table>
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</div>
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Revision as of 19:46, 10 October 2017

Methods

Protocols

Enzymes

Digestion enzymes

Enzyme Producer
AgeI-HF NEB
BsaI NEB
DpnI NEB
DpnI Jena Bioscience
EciI NEB
EcoRV Fermentas
MnlI NEB
NotI Jena Bioscience
PstI NEB
PstI Thermo Fisher Scientific
PstI-HF NEB
SapI NEB
SpeI NEB
XbaI NEB
XbaI Jena Bioscience

Polymerases

Enzyme Producer
Go Taq DNA polymerase Promega
Klenow Fragment Thermo Fisher Scientific
Phusion High-Fidelity DNA polymerase NEB
Q5 High-Fidelity DNA polymerase NEB
Taq Polymerase Jena Bioscience

Other Enzymes

Enzyme Producer
Shrimp Alkaline Phosphatase Jena Bioscience
T4 DNA Ligase NEB
T4 DNA Ligase Thermo Fisher Scientific
T4 Polynucleotide Kinase NEB