Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Methods"
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<h3> Protocols </h3> | <h3> Protocols </h3> | ||
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| + | This page lists all regular methods used during our project. Special methods, which are not listed here, are described or linked on the corresponding result pages. | ||
| + | </div> | ||
<div class="contentline"> | <div class="contentline"> | ||
<div class="half left"> | <div class="half left"> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--protocol_biotynilation.pdf" target="blank">Biotynilation of glass slides</a></li> |
| + | <li><a href="https://static.igem.org/mediawiki/2017/e/e8/T--Bielefeld-CeBiTec--protocol_biotinylierung_of_glass_slides.pdf" target="blank">Biotinylation of glass slides</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/6/6f/T--Bielefeld-CeBiTec--RotiNanoquant.pdf">Bradford estimation using RotiNanoquant</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/9/97/T--Bielefeld-CeBiTec--protocol_cloning_strategy_re.pdf" target="blank">Cloning Strategy with Restriction Enzymes</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | ||
| − | |||
<li><a href="https://static.igem.org/mediawiki/2017/4/42/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_buffer.pdf" target="blank">Fast cell lysis sample buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/4/42/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_buffer.pdf" target="blank">Fast cell lysis sample buffer</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/e/e0/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_SDS-Page.pdf" target="blank">Fast cell lysis for SDS-PAGE</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/e/e0/T--Bielefeld-CeBiTec--protocol_fast_cell_lysis_SDS-Page.pdf" target="blank">Fast cell lysis for SDS-PAGE</a></li> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/4/42/T--Bielefeld-CeBiTec--protocol_generating_chemocompetent_cells.pdf" target="blank">Generating chemocompetent cells</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/4/42/T--Bielefeld-CeBiTec--protocol_generating_chemocompetent_cells.pdf" target="blank">Generating chemocompetent cells</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/6/6c/T--Bielefeld-CeBiTec--protocol_generating_electrocompetent_cells.pdf" target="blank">Generating electrocompetent cells</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/6/6c/T--Bielefeld-CeBiTec--protocol_generating_electrocompetent_cells.pdf" target="blank">Generating electrocompetent cells</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/3/37/T--Bielefeld-CeBiTec--protocol_genome_editing_codA.pdf">Genome editing via the CRISP-Cas9 system</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/5/55/T--Bielefeld-CeBiTec--protocol_gibson_assembly.pdf" target="blank">Gibson Assembly</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/5/55/T--Bielefeld-CeBiTec--protocol_gibson_assembly.pdf" target="blank">Gibson Assembly</a></li> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/7/75/T--Bielefeld-CeBiTec--protocol_innuPREP_Gel_analytikJena.pdf" target="blank">innuPREP Gel Extraktion Kit (Analytik Jena)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/7/75/T--Bielefeld-CeBiTec--protocol_innuPREP_Gel_analytikJena.pdf" target="blank">innuPREP Gel Extraktion Kit (Analytik Jena)</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/8/8c/T--Bielefeld-CeBiTec--YKE_lycopene_protocol.pdf" target="blank">Lycopene extraction</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/a/a7/T--Bielefeld-CeBiTec--protocol_LB.pdf" target="blank">Lysogeny Broth (LB)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/a/a7/T--Bielefeld-CeBiTec--protocol_LB.pdf" target="blank">Lysogeny Broth (LB)</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/8/8a/T--Bielefeld-CeBiTec--protocol_Monarch_Miniprep_kit_NEB.pdf" target="blank">Monarch® Plasmid Miniprep Kit (NEB)</a></li> |
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</ul> | </ul> | ||
</div> | </div> | ||
<div class="half right"> | <div class="half right"> | ||
<ul style="list-style-type: none"> | <ul style="list-style-type: none"> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--protocol_Monarch_PCR_and_DNA_clean_up_kit_NEB.pdf" target="blank">Monarch® PCR & DNA Cleanup Kit (NEB)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--protocol_Monarch_PCR_and_DNA_clean_up_kit_NEB.pdf" target="blank">Monarch® PCR & DNA Cleanup Kit (NEB)</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/7/7d/T--Bielefeld-CeBiTec--protocol_MOPS.pdf" target="blank">MOPS Minimal Medium</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/7/7d/T--Bielefeld-CeBiTec--protocol_MOPS.pdf" target="blank">MOPS Minimal Medium</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--protocol_native_PAGE.pdf" target="blank">Native DNA PAGE</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--protocol_native_PAGE.pdf" target="blank">Native DNA PAGE</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/6/63/T--Bielefeld-CeBiTec--protocol_PBJR_buffer.pdf" target="blank">PBJR buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/6/63/T--Bielefeld-CeBiTec--protocol_PBJR_buffer.pdf" target="blank">PBJR buffer</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/2/2b/T--Bielefeld-CeBiTec--ProtocolPhusion.pdf" target="blank">PCR with Phusion High Fidelity Master Mix with HC enhancer</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/8/88/T--Bielefeld-CeBiTec--protocol_PCR_Q5_HF_Polymerase.pdf" target="blank">PCR with Q5 High-Fidelity Polymerase</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/88/T--Bielefeld-CeBiTec--protocol_PCR_Q5_HF_Polymerase.pdf" target="blank">PCR with Q5 High-Fidelity Polymerase</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/3/33/T--Bielefeld-CeBiTec--protocol_golden_gate.pdf" target="blank">Plasmid Assembly Protocol with Golden Gate Assembly</a></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--ImpactProtocol.pdf">Protein Purification Impact Protocol</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/9/96/T--Bielefeld-CeBiTec--protocol_PCR_PureYield_PlasmidMiniprep_Promega.pdf" target="blank">PureYield<sup>TM</sup> Plasmid Miniprep System (Promega)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/9/96/T--Bielefeld-CeBiTec--protocol_PCR_PureYield_PlasmidMiniprep_Promega.pdf" target="blank">PureYield<sup>TM</sup> Plasmid Miniprep System (Promega)</a></li> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/0/0d/T--Bielefeld-CeBiTec--protocol_SDS-PAGE.pdf" target="blank">SDS-PAGE</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/a/a2/T--Bielefeld-CeBiTec--protocol_SDS-PAGE_running_buffer.pdf" target="blank">SDS-PAGE running buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/a/a2/T--Bielefeld-CeBiTec--protocol_SDS-PAGE_running_buffer.pdf" target="blank">SDS-PAGE running buffer</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--protocol_SOC.pdf" target="blank">SOC-medium</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--protocol_SOC.pdf" target="blank">SOC-medium</a></li> | ||
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<li><a href="https://static.igem.org/mediawiki/2017/8/84/T--Bielefeld-CeBiTec--protocol_Standard_Biobrick.pdf" target="blank">Standard BioBrick Assembly</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/84/T--Bielefeld-CeBiTec--protocol_Standard_Biobrick.pdf" target="blank">Standard BioBrick Assembly</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/d/d4/T--Bielefeld-CeBiTec--protocol_TAE_buffer.pdf" target="blank">TAE buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/d/d4/T--Bielefeld-CeBiTec--protocol_TAE_buffer.pdf" target="blank">TAE buffer</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/a/ab/T--Bielefeld-CeBiTec--protocol_electrotrafo.pdf" target="blank">Transformation via electroporation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/a/a0/T--Bielefeld-CeBiTec--protocol_heatshock.pdf" target="blank">Transformation via heat shock</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/2/23/T--Bielefeld-CeBiTec--protocol_Restriction_Digest.pdf" target="blank">"Typical" Restriction Digest</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/2/2e/T--Bielefeld-CeBiTec--protocol_PCR_Gel_Cleanup_wizzard.pdf" target="blank">Wizard® SV Gel and PCR Clean-Up System (Promega)</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--protocol_ZymoPURETM_PlasmidMiniprep_Kit.pdf" target="blank">ZymoPURE<sup>TM</sup> Plasmid Miniprep Kit (Zymo Research)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--protocol_ZymoPURETM_PlasmidMiniprep_Kit.pdf" target="blank">ZymoPURE<sup>TM</sup> Plasmid Miniprep Kit (Zymo Research)</a></li> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 15:49, 1 November 2017
Methods
Protocols
This page lists all regular methods used during our project. Special methods, which are not listed here, are described or linked on the corresponding result pages.
- 50xTAE Stock Solution
- Acetone Precipitation
- Biotynilation of glass slides
- Biotinylation of glass slides
- Blunt end ligation
- Bradford estimation using RotiNanoquant
- Cloning Strategy with Restriction Enzymes
- Colony PCR GoTaq® G2 DNA Polymerase
- Fast cell lysis sample buffer
- Fast cell lysis for SDS-PAGE
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- Generating chemocompetent cells
- Generating electrocompetent cells
- Genome editing via the CRISP-Cas9 system
- Gibson Assembly
- innuPREP Gel Extraktion Kit (Analytik Jena)
- Lycopene extraction
- Lysogeny Broth (LB)
- Monarch® Plasmid Miniprep Kit (NEB)
- Monarch® PCR & DNA Cleanup Kit (NEB)
- MOPS Minimal Medium
- Native DNA PAGE
- PBJR buffer
- PCR with Phusion High Fidelity Master Mix with HC enhancer
- PCR with Q5 High-Fidelity Polymerase
- Plasmid Assembly Protocol with Golden Gate Assembly
- Protein Purification Impact Protocol
- PureYieldTM Plasmid Miniprep System (Promega)
- SDS-PAGE
- SDS-PAGE running buffer
- SOC-medium
- ssDNA Annealing
- Standard BioBrick Assembly
- TAE buffer
- Transformation via electroporation
- Transformation via heat shock
- "Typical" Restriction Digest
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- ZymoPURETM Plasmid Miniprep Kit (Zymo Research)
Enzymes
Digestion enzymes
| Enzyme | Producer |
|---|---|
| AgeI-HF | NEB |
| BsaI | NEB |
| DpnI | NEB |
| DpnI | Jena Bioscience |
| EciI | NEB |
| EcoRV | Fermentas |
| MnlI | NEB |
| NotI | Jena Bioscience |
| PstI | NEB |
| PstI | Thermo Fisher Scientific |
| PstI-HF | NEB |
| SapI | NEB |
| SpeI | NEB |
| XbaI | NEB |
| XbaI | Jena Bioscience |
Polymerases
| Enzyme | Producer |
|---|---|
| Go Taq DNA polymerase | Promega |
| Klenow Fragment | Thermo Fisher Scientific |
| Phusion High-Fidelity DNA polymerase | NEB |
| Q5 High-Fidelity DNA polymerase | NEB |
| Taq Polymerase | Jena Bioscience |
Other Enzymes
| Enzyme | Producer |
|---|---|
| Shrimp Alkaline Phosphatase | Jena Bioscience |
| T4 DNA Ligase | NEB |
| T4 DNA Ligase | Thermo Fisher Scientific |
| T4 Polynucleotide Kinase | NEB |
