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| <h2> Toolbox </h2> | | <h2> Toolbox </h2> |
| <article> | | <article> |
− | Our toolbox for advanced protein design will consist of seven different tRNA/aminacyl-synthetases (aaRS), each capable of loading a specific non‑canonical amino acid (ncAA) to the amber tRNA or the less used leucine tRNA to incorporate these ncAAs into all kinds of target proteins. The aaRSs will be provided as parts in the Registry of Standard Biological Parts and hopefully integrated in the distribution, so that further iGEM teams will have the benefit of using our toolbox to improve their projects. The properties we would like to integrate in the toolbox are<a href"https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing"> analyzing</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">photoswitching</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">labeling</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">photolysis</a> and <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">fusing</a> (Figure 1). | + | Our toolbox for advanced protein design will consist of seven different tRNA/aminacyl-synthetases (aaRS), each capable of loading a specific noncanonical amino acid (ncAA) to the amber tRNA or the less used leucine tRNA to incorporate these ncAAs into all kinds of target proteins. The aaRSs will be provided as parts in the Registry of Standard Biological Parts and hopefully integrated in the distribution, so that further iGEM teams will have the benefit of using our toolbox to improve their projects. The properties we would like to integrate in the toolbox are<a href"https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing"> analyzing</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">photoswitching</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">labeling</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">photolysis</a> and <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">fusing</a> (Figure 1). |
| </article> | | </article> |
| <div class="figure small"> | | <div class="figure small"> |
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| <h2>Two different ways on how to incorporate ncAAs</h2> | | <h2>Two different ways on how to incorporate ncAAs</h2> |
| <article> | | <article> |
− | Generally the incorporation in response to the amber stop codon (TAG) and the less used leucine codon (CTA) limits the number of different non canonical amino acids to two. The incorporation through these codons also inhibits the growth of culture, like described in the translation mechanism. An additional base in the DNA would circumvent these problems and offer 64 new different codons to incorporate different ncAAs like shown in Figure 2. Both ways, the incorporation through codons containing an unnatural base and the incorporation through a less used existing codon could be used to incorporate ncAAs. | + | Generally the incorporation in response to the amber stop codon (TAG) and the less used leucine codon (CTA) limits the number of different noncanonical amino acids to two. The incorporation through these codons also inhibits the growth of culture, like described in the translation mechanism. An additional base in the DNA would circumvent these problems and offer 64 new different codons to incorporate different ncAAs like shown in Figure 2. Both ways, the incorporation through codons containing an unnatural base and the incorporation through a less used existing codon could be used to incorporate ncAAs. |
| </article> | | </article> |
| <div class="figure small"> | | <div class="figure small"> |