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| <h2> Toolbox </h2> | | <h2> Toolbox </h2> |
| <article> | | <article> |
− | Our toolbox for advanced protein design consists of eight different tRNA/aminacyl-synthetases (aaRS), each capable of loading a specific non-canonical amino acid (ncAA) to the amber tRNA or the less used leucine tRNA to incorporate these ncAAs into all kinds of target proteins. The aaRSs are provided as parts in the Registry of Standard Biological Parts and hopefully integrated in the distribution, so that further iGEM teams will have the benefit of using our toolbox to improve their projects. The properties we would like to integrate in the toolbox are<a href"https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing"> analyzing</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">photoswitching</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">labeling</a>, <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">photolysis</a> and <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">fusing</a> (Figure 1). | + | Our toolbox for advanced protein design consists of eight different tRNA/aminacyl-synthetases (aaRS), each capable of loading a specific non-canonical amino acid (ncAA) to the amber tRNA or the less used leucine tRNA to incorporate these ncAAs into all kinds of target proteins. The aaRSs are provided as parts in the Registry of Standard Biological Parts and hopefully integrated in the distribution, so that further iGEM teams will have the benefit of using our toolbox to improve their projects. The properties we would like to integrate in the toolbox are<a target="_blank" href"https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing"> analyzing</a>, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">photoswitching</a>, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">labeling</a>, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">photolysis</a> and <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">fusing</a> (Figure 1). |
| </article> | | </article> |
| <div class="figure small"> | | <div class="figure small"> |
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| <article> | | <article> |
| <ul> | | <ul> |
− | <li> <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing">Analyzing</a> (<a href="http://parts.igem.org/Part:BBa_K2201201">BBa_K2201201</a>, <a href="http://parts.igem.org/Part:BBa_K2201202">BBa_K2201202</a>): Two different ncAAs are incorporated which are labeled with fluorophores in a chemically reaction. With the help of Foerster Resonance Energy Transfer (FRET) the distance between the amino acids could be measured. | + | <li> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing">Analyzing</a> (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201201">BBa_K2201201</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201202">BBa_K2201202</a>): Two different ncAAs are incorporated which are labeled with fluorophores in a chemically reaction. With the help of Foerster Resonance Energy Transfer (FRET) the distance between the amino acids could be measured. |
− | <li> <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">Photoswitching</a> (<a href="http://parts.igem.org/Part:BBa_K2201207">BBa_K2201207</a>): A photoisomerisable amino acid could be incorporated which changes its conformation when it is irradiated with light of different wavelengths. In one conformation it inhibits the function of the target protein. With this photoswitch, reactions could be switched on and off on the protein level only by light exposure. | + | <li> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photoswitching">Photoswitching</a> (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201207">BBa_K2201207</a>): A photoisomerisable amino acid could be incorporated which changes its conformation when it is irradiated with light of different wavelengths. In one conformation it inhibits the function of the target protein. With this photoswitch, reactions could be switched on and off on the protein level only by light exposure. |
− | <li> <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">Labeling</a> (<a href="http://parts.igem.org/Part:BBa_K2201204">BBa_K2201204</a>): A fluorescent amino acid could be used to label the target protein <i>in vivo</i>. The advantages compared to fluorescent proteins lay in the smaller size of the fluorescent amino acid. | + | <li> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/labeling">Labeling</a> (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201204">BBa_K2201204</a>): A fluorescent amino acid could be used to label the target protein <i>in vivo</i>. The advantages compared to fluorescent proteins lay in the smaller size of the fluorescent amino acid. |
− | <li> <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">Photolysis</a> (<a href="http://parts.igem.org/Part:BBa_K2201200">BBa_K2201200</a>): Photolysis amino acids break the peptide backbone at the position they were incorporated. This could be used to activate or deactivate proteins by cleaving them. | + | <li> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/photolysis">Photolysis</a> (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201200">BBa_K2201200</a>): Photolysis amino acids break the peptide backbone at the position they were incorporated. This could be used to activate or deactivate proteins by cleaving them. |
− | <li> <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">Fusing</a> (<a href="http://parts.igem.org/Part:BBa_K2201205">BBa_K2201205</a>, <a href="http://parts.igem.org/Part:BBa_K2201206">BBa_K2201206</a>): Two ncAAs which could form a specific covalent bond to each other could be used to fuse proteins together or immobilize the target protein not only at the C- or N-termini. | + | <li> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/fusing">Fusing</a> (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201205">BBa_K2201205</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201206">BBa_K2201206</a>): Two ncAAs which could form a specific covalent bond to each other could be used to fuse proteins together or immobilize the target protein not only at the C- or N-termini. |
| </ul> | | </ul> |
| <br> | | <br> |