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+ | <!-- Ueberschriften --> | ||
+ | <h2> Translational System Overview </h2> | ||
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+ | The genetic information, coded in the DNA, are the basis for the expression of proteins and therefore are essential for all living organisms. | ||
+ | The expansion of the genetic code, by adding new bases to the DNA, offers multiple possibilities, like the coding of non-canonical amino acids. At the same time, it is going to create new challenges. | ||
+ | Beside a preservation system for the <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/unnatural_base_pair/unnatural_base_pairs">unnatural-base pairs (UBP)</a>, the whole transcriptional and translational system has to be adapted if a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox">non-canonical amino acid (ncAA)</a> is coded by UBPs. | ||
+ | The incorporation of ncAA generates many new properties of proteins and therefore a wide specter of applications. It can be achieved either by the use of UBPs or coded by an <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/translational_system/translation_mechanism">amber-stop codon<a/>. | ||
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+ | The incorporation of a ncAA by an amber-stop codon requires the recognition of the codon and a tRNA/aminoacyl-synthetase (tRNA/aaRS) pair which is able to accept and bind the ncAA (to charge the tRNA with the ncAA). | ||
+ | In addition, the tRNA/aaRS pair should be very specific in the incorporation of the ncAA. Therefore a library of the mutated <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/translational_system/translation_mechanism">orthogonal tRNA/aaRS</a> is generated and undergoes numerous rounds of positive and negative <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/translational_system/library_and_selection">selection</a> for the adaption of the codon recognition and the amino acid binding. The selection results in a synthetase which can be expressed efficiently in <i>E. coli</i> and is able to reliable incorporate an unnatural amino acid. | ||
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Revision as of 07:10, 11 October 2017
Translational System