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The expansion of the genetic code, by adding new bases to the DNA, offers multiple possibilities, like the coding of non-canonical amino acids. At the same time, it is going to create new challenges. | The expansion of the genetic code, by adding new bases to the DNA, offers multiple possibilities, like the coding of non-canonical amino acids. At the same time, it is going to create new challenges. | ||
Beside a preservation system for the <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/unnatural_base_pair/unnatural_base_pairs">unnatural-base pairs (UBP)</a>, the whole transcriptional and translational system has to be adapted if a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox">non-canonical amino acid (ncAA)</a> is coded by UBPs. | Beside a preservation system for the <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/unnatural_base_pair/unnatural_base_pairs">unnatural-base pairs (UBP)</a>, the whole transcriptional and translational system has to be adapted if a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox">non-canonical amino acid (ncAA)</a> is coded by UBPs. | ||
− | The incorporation of ncAA generates many new properties of proteins and therefore a wide specter of applications. It can be achieved either by the use of UBPs or coded by an <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/translational_system/translation_mechanism">amber-stop codon< | + | The incorporation of ncAA generates many new properties of proteins and therefore a wide specter of applications. It can be achieved either by the use of UBPs or coded by an <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/translational_system/translation_mechanism">amber-stop codon</a>. |
The incorporation of a ncAA by an amber-stop codon requires the recognition of the codon and a tRNA/aminoacyl-synthetase (tRNA/aaRS) pair which is able to accept and bind the ncAA (to charge the tRNA with the ncAA). | The incorporation of a ncAA by an amber-stop codon requires the recognition of the codon and a tRNA/aminoacyl-synthetase (tRNA/aaRS) pair which is able to accept and bind the ncAA (to charge the tRNA with the ncAA). |
Revision as of 09:03, 11 October 2017
Translational System Overview