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Our aim was to provide an innovative <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox">toolbox</a> to incorporate different non-canonical amino acids (ncAAs) during translation to expand the possibilities in protein design. We chose seven different ncAAs for five tools and demonstrated interesting applications for them. These ncAAs can be used for various approaches in basic research, medicine and manufacturing. Furthermore, with our submitted parts, every iGEM team can incorporate these seven ncAAs into their target proteins. A short description of every tool is listed below. | Our aim was to provide an innovative <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox">toolbox</a> to incorporate different non-canonical amino acids (ncAAs) during translation to expand the possibilities in protein design. We chose seven different ncAAs for five tools and demonstrated interesting applications for them. These ncAAs can be used for various approaches in basic research, medicine and manufacturing. Furthermore, with our submitted parts, every iGEM team can incorporate these seven ncAAs into their target proteins. A short description of every tool is listed below. |
Latest revision as of 03:03, 2 November 2017
Toolbox Overview
Short Summary
Analyzing
The structural analysis of a protein can be used to study distances between non-canonical amino acids (ncAAs) with Foerster Resonance Energy Transfer (FRET). This provides measuring distances between specific incorporated amino acids in the target protein. To incorporate the ncAAs we provide three different tRNA/aminoacyl-synthetases (BBa_K2201201, BBa_K2201202, BBa_K2201203) which incorporate in response to the amber or the less used leucine codon. To demonstrate this tool we developed a prion detection assay.