Difference between revisions of "Team:Bulgaria/Contribution"

 
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                 <a href="https://2017.igem.org/Team:Bulgaria/public-engagement">Public Engagements</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/public-engagement">Public Engagements</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/i-engineer" style="border-top:1px solid black">&nbsp; I, Engineer</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/i-engineer" style="border-top:1px solid black">&nbsp; I, Engineer</a>
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                 <a href="https://2017.igem.org/Team:Bulgaria/fission-festival">&nbsp; Fission festival</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/fission-festival">&nbsp; Fission festival</a>
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                 <a href="https://2017.igem.org/Team:Bulgaria/international-workshop-innovation-corner">&nbsp; International workshop Innovation corner</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/international-workshop-innovation-corner">&nbsp; International workshop Innovation corner</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/sofia-science-festival">&nbsp; Sofia science festival</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/sofia-science-festival">&nbsp; Sofia science festival</a>
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                 <a href="https://2017.igem.org/Team:Bulgaria/researchers-night" >&nbsp; The European Researchers’ Night</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/researchers-night" >&nbsp; The European Researchers’ Night</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/bloomberg-tv-bulgaria">&nbsp; Bloomberg TV Bulgaria</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/bloomberg-tv-bulgaria">&nbsp; Bloomberg TV Bulgaria</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/bnt1-tv-bulgaria">&nbsp; BNT1 TV Bulgaria</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/agar-art-bulgaria">&nbsp; Agar Art - Bulgaria</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/agar-art-bulgaria">&nbsp; Agar Art - Bulgaria</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/bg-science-magazine" style="border-bottom:1px solid black">&nbsp; BG Economics Magazine</a>
 
                 <a href="https://2017.igem.org/Team:Bulgaria/bg-science-magazine" style="border-bottom:1px solid black">&nbsp; BG Economics Magazine</a>
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         <a href="https://2017.igem.org/Team:Bulgaria/Collaborations">Collaborations</a>
 
         <a href="https://2017.igem.org/Team:Bulgaria/Collaborations">Collaborations</a>
 
         <a href="https://2017.igem.org/Team:Bulgaria/safety">Safety</a>
 
         <a href="https://2017.igem.org/Team:Bulgaria/safety">Safety</a>
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        <a href="https://2017.igem.org/Team:Bulgaria/acknowledgements">Acknowlegements</a>
 
 
         <a href="https://2017.igem.org/Team:Bulgaria/Attributions">Attributions</a>
 
         <a href="https://2017.igem.org/Team:Bulgaria/Attributions">Attributions</a>
  
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Latest revision as of 02:46, 2 November 2017


Home Team
Description Design Experiments Results Contribution Improve Second project Demonstrate Lab book Protocols
Silver Gold Survey Public Engagements   I, Engineer   Fission festival   International workshop Innovation corner   Sofia science festival   The European Researchers’ Night   Bloomberg TV Bulgaria   BNT1 TV Bulgaria   Agar Art - Bulgaria   BG Economics Magazine Project Yana Project Vyara
Parts
Collaborations Safety Attributions


Contribution


Bronze medal contribution


We assembled a test construct consisting of INPUT TetR constitutive generator (Part: BBa_K145201), Ptet promoter and a GFP generator. We made this construct since we wanted to test and optimize the induction of the pTet promoter. In course of our work we noticed that GFP is produced in high amounts even without an induction. The explanation we found most logical was that the TetR protein has a degradation tag (we found one that is not described in part BBa_K145201 but exists in the sequence) and it is not stable enough. So we decided to modify and improve this part. To do so we PCR amplify the part in a way that the degradation tag is removed and the TetR constitutive generator is in the opposite direction (limiting the chance for incidental transcription events). Our new part was characterized and submitted to the Registry.

Other contributions


1) We designed a BioBrick compatible gRNA vector and submitted it to the registry.


2) We tested and optimized the gRNA cloning process.