Difference between revisions of "Team:Bulgaria/Results"

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{{Bulgaria}}
 
 
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            text-decoration: none !important;
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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            position: absolute !important;
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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        }
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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</ul>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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            text-transform: uppercase !important;
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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        }
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</ul>
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        p{font-size:large !important;}
  
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</head>
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<body>
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<div class="container">
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    <div class=" mynavbar">
  
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        <a href="https://2017.igem.org/Team:Bulgaria">Home</a>
 +
        <a href="https://2017.igem.org/Team:Bulgaria/team">Team</a>
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            <button class=" mydropbtn">Project
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                <a href="https://2017.igem.org/Team:Bulgaria/description" style="border-bottom:1px solid black">Description</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/design" style="border-bottom:1px solid black">Design</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/experiments" style="border-bottom:1px solid black">Experiments</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/results" style="border-bottom:1px solid black">Results</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/contribution" style="border-bottom:1px solid black">Contribution</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/improve" style="border-bottom:1px solid black">Improve</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/second-project" style="border-bottom:1px solid black">Second project</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/Demonstrate" style="border-bottom:1px solid black">Demonstrate</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/lab-book" style="border-bottom:1px solid black">Lab book</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/protocols">Protocols</a>
 +
            </div>
 +
        </div>
  
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<h5>Inspiration</h5>
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        <div class=" mydropdown">
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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</ul>
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            <button class=" mydropbtn">Human Practices
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                <a href="https://2017.igem.org/Team:Bulgaria/HP/Silver" style="border-bottom:1px solid black">Silver</a>
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                <a href="https://2017.igem.org/Team:Bulgaria/HP/Gold_Integrated" style="border-bottom:1px solid black">Gold</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/survey" style="border-bottom:1px solid black">Survey</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/public-engagement">Public Engagements</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/i-engineer" style="border-top:1px solid black">&nbsp; I, Engineer</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/fission-festival">&nbsp; Fission festival</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/international-workshop-innovation-corner">&nbsp; International workshop Innovation corner</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/sofia-science-festival">&nbsp; Sofia science festival</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/researchers-night" >&nbsp; The European Researchers’ Night</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/bloomberg-tv-bulgaria">&nbsp; Bloomberg TV Bulgaria</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/bnt1-tv-bulgaria">&nbsp; BNT1 TV Bulgaria</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/agar-art-bulgaria">&nbsp; Agar Art - Bulgaria</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/bg-science-magazine" style="border-bottom:1px solid black">&nbsp; BG Economics Magazine</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/project-yana" style="border-bottom:1px solid black">Project Yana</a>
 +
                <a href="https://2017.igem.org/Team:Bulgaria/project-vyara">Project Vyara</a>
 +
            </div>
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            <button class=" mydropbtn">Achievements
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                <a href="https://2017.igem.org/Team:Bulgaria/parts" >Parts</a>
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            </div>
 +
        </div>
 +
        <a href="https://2017.igem.org/Team:Bulgaria/Collaborations">Collaborations</a>
 +
        <a href="https://2017.igem.org/Team:Bulgaria/safety">Safety</a>
 +
        <a href="https://2017.igem.org/Team:Bulgaria/Attributions">Attributions</a>
 +
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    </div>
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<br>
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<br>
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<div class="row">
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    <div class="col-md-12">
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        <h1 style="color:#1AA55E; font-family: Comic sans MS;"> Results </h1>
 +
        <br>
 +
      <h3>
 +
          <b>gRNA vector</b>
 +
      </h3>
 +
        <p><br />
 +
 +
 +
            We created a gRNA expression vector gRNA-pSB1C3 (BBa_K2515002). We successfully cloned our gRNAs into it and confirmed their functionality via transformatin in <i>E. coli</i> competent cells with Cas9. As expected no colonies were obtained. <br /><br />
 +
 +
<a href="https://imgur.com/VopDgWt"><img src="https://i.imgur.com/VopDgWt.jpg" title="source: imgur.com" /></a>
 +
<br /><br />
 +
 +
Then we cloned a couple of gRNAs for the <i>ftsZ</i> gene. Upon arabinose induction we observed a trend to elongated cellular shape (see the picture below - left is ftsZ gRNA, right - control). This was a positive trend no matter that we expected a stronger phenotype based on literature data.<br /><br />
 +
<a href="https://imgur.com/uIQzjyu"><img src="https://i.imgur.com/uIQzjyu.jpg" title="source: imgur.com" /></a>
 +
<br /><br />
 +
 +
This observation was supported by the fact that ftsZ gRNA containing cultures develop much more intensive red color (see the picture below - 3 left tubes are ftsZ gRNAs, right - control). The more intense color can be explained with inhibition of the cellular division to a given extent and redirection of the metabolic flux to side processes like chromoprotein production.<br /><br />
 +
<a href="https://imgur.com/APeObPg"><img src="https://i.imgur.com/APeObPg.jpg" title="source: imgur.com" /></a><br /><br />
 +
 +
 
 +
        </p>
 +
 +
        <h3>
 +
            <b> Mutator Strain</b>
 +
        </h3>
 +
        <p><br /><br />
 +
            We tested our mutator strain via plating cultures with gRNA arrays that suppress genes with an important role in different reparation and/or replication fidelity systems. As a readout we used the number of rifampicin resistant colonies. Unfortunately our results so far are inconclusive due to the small experiment number. We hope that this will change until our presentation on the Giant Jamboree.
 +
        </p>
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    </div>
 
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Latest revision as of 02:47, 2 November 2017

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gRNA vector


We created a gRNA expression vector gRNA-pSB1C3 (BBa_K2515002). We successfully cloned our gRNAs into it and confirmed their functionality via transformatin in E. coli competent cells with Cas9. As expected no colonies were obtained.



Then we cloned a couple of gRNAs for the ftsZ gene. Upon arabinose induction we observed a trend to elongated cellular shape (see the picture below - left is ftsZ gRNA, right - control). This was a positive trend no matter that we expected a stronger phenotype based on literature data.



This observation was supported by the fact that ftsZ gRNA containing cultures develop much more intensive red color (see the picture below - 3 left tubes are ftsZ gRNAs, right - control). The more intense color can be explained with inhibition of the cellular division to a given extent and redirection of the metabolic flux to side processes like chromoprotein production.



Mutator Strain



We tested our mutator strain via plating cultures with gRNA arrays that suppress genes with an important role in different reparation and/or replication fidelity systems. As a readout we used the number of rifampicin resistant colonies. Unfortunately our results so far are inconclusive due to the small experiment number. We hope that this will change until our presentation on the Giant Jamboree.