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gRNA vector


We created a gRNA expression vector gRNA-pSB1C3 (BBa_K2515002). (.



A careful sequence analysis reviled that a LVA-like degradation tag exists in the reading frame of TetR in BBa_K145201. Moreover, the J23116 promoter is in the same orientation as the downstream expression unit. Indeed, they are separated by a transcription terminator but it is known that no terminator is a 100% effective.

To improve this part, we used a PCR based approach, using BBa_K145201 as matrix. The resulting new part (BBa_K2515003) was cloned in pSB1C3 and submitted to the registry. Our new part has TetR without a degradation tag and is in inverted orientation compared to the original one. Thanks to that the basal uninduced expression levels can be lowered.

See our new part here.

Gold Medal Criterion #2



We offer a stratee gRNAs.