Difference between revisions of "Team:CGU Taiwan/Hardware"

 
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Results">RESULTS</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Results">RESULTS</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/InterLab">INTERLAB</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/InterLab">INTERLAB</a>
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       <a href="#">PROOF</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Demonstrate">PROOF AND DEMONSTRATION</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Model">MODEL</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Model">MODEL</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Parts">PART</a>    
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Parts">PARTS</a>
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      <a href="https://2017.igem.org/Team:CGU_Taiwan/Future">FUTURE WORK</a>     
 
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       <div class="dropdown-content">
 
       <div class="dropdown-content">
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Notebook">LAB NOTE</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Notebook">LAB NOTE</a>
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       <a href="#">PROTOCOLS</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Protocols">PROTOCOLS</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Safety">SATEFY</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Safety">SAFETY</a>
 
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   <li class="dropdown"><a href="Team">TEAM</a>
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   <li class="dropdown"><a href="https://2017.igem.org/Team:CGU_Taiwan/Team">TEAM</a>
 
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       <div class="dropdown-content">
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Attributions">ATTRIBUTION</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Attributions">ATTRIBUTION</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Team">MEMBER</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Team">MEMBER</a>
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       <a href="#">SPONSOR</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Acknowledgement">ACKNOWLEDGEMENT</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Collaborations">COLLBORATION</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Collaborations">COLLABORATION</a>
 
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/HP/Gold_Integrated">INTERGRATED AND GOLD</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/HP/Gold_Integrated">INTERGRATED AND GOLD</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Engagement">PUBLIC ENGAGEMENT</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Engagement">PUBLIC ENGAGEMENT</a>
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      <a href="https://2017.igem.org/Team:CGU_Taiwan/TWConference">2017 iGEM TAIWAN CONFERENCE</a>
 
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  <li class="dropdown"><a href="">AWARD</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Applied_Design">APPLIED DESIGN</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Entrepreneurship">ENTREPRENEURSHIP</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Entrepreneurship">ENTREPRENEURSHIP</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Hardware">HARDWARE</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Hardware">HARDWARE</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Measurement">MEASUREMENT</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Measurement">MEASUREMENT</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Model">MODEL</a>
 
       <a href="https://2017.igem.org/Team:CGU_Taiwan/Model">MODEL</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Plant">PLANT</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/InterLab">INTERLAB</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/Software">SOFTWARE</a>
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       <a href="https://2017.igem.org/Team:CGU_Taiwan/HP/Gold_Integrated">INTERGRATED AND GOLD</a>
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      <a href="https://2017.igem.org/Team:CGU_Taiwan/Engagement">PUBLIC ENGAGEMENT</a>
 
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<!-- Project Description start -->
 
<!-- Project Description start -->
 
<div class="description" style="text-align:center">
 
<div class="description" style="text-align:center">
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<h1>Introduction</h1>
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<h1>Hardware</h1>
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<p style="font-size:150%"> To achieve the localize deinking, we built a system that can detect the ink on the paper and using red light LED matrix to stimulate the yeast in some specific locations to produce enzymes.
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<img src="https://static.igem.org/mediawiki/2017/c/c0/Cgu_hardware_p1.png" width="50%"><br>
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The paper will be photographed and sent to computer before we spread the yeast on it. Our image process program using OpenCV function library to decode the JPG image file and do the edge offset, then decide which area have ink. After the LED matric data be calculated, the program using RS-232 serial communication standard sends this data to Arduino. Arduino collects the data and then sends it to Max7219 microcontroller to light up the LED matrix to stimulate the yeast produce enzymes.
+
<p style="font-size:150%"> It is localizing the reaction of enzyme on the paper that can reach two goals, reducing the pollution from deinking and maintaining the fibers without inks to keep the strength of the reprocessed paper. Thus, we have created a new device to reform the procedure to make reprocessed paper. Briefly describing the function of the device, the paper will be drew into the box and taken a picture to be analyzed the distribution of the surface area of ink. Then, the engineered yeast will be sprayed on the ink part on the paper to be induced to secrete out enzyme only on the ink part. Thus, the enzyme can be precisely working on the ink part of the paper to preserve the other fibers without the ink on the paper. Additionally, our concept has been confirmed by the pro
−
The system accepts any shape of paper which is under the size of the device, and the device can be easily scale up by connecting more led matric. This shows the possibility of factory scale localize deinking. </p>
+
cedure we made reprocessed paper in lab. We believe our enzymatic deinking process can fulfill our dream with this innovative device.
 +
</p>
 +
<h1>Structure</h1>
 +
<p style="font-size:150%"> The structure of the main part is made of acrylic sheets which are joined with chloroform. Since chloroform welds plastic, it gives the device well stability. For the LED lights, circuit boards are stablized on acrylic sheet drilled.</p>
 +
 
 +
<h1>Component Description</h1>
 +
<p style="font-size:150%"> In the following paragraphs, our device will be dismantled into single components and their functions will be explained respectively.
 +
</p>
 +
<h2>Case</h2>
 +
<p style="font-size:150%"> Our device is like an opaque box with a cover on the top and two drawers. Opaque box with internal dimension about 18 cm in width, 20 cm in length and 19 cm in height offers a dim environment which avoids the yeast being induced out of control.</p>
 +
<img src="https://static.igem.org/mediawiki/2017/f/f1/Cgu_hardware_case_p1.png" width="70%">
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2017/f/f1/Cgu_hardware_case_p2.png" width="70%">
 +
<h2>Baseplate</h2>
 +
<p style="font-size:150%"> It is located at the bottom of the device. Paper will be placed on it and sent into the case to be analyzed and deinked. The length and width of the plate are both the same as  the LED matrix, so paper that is not oversized can apply to the machine.</p>
 +
<img src="https://static.igem.org/mediawiki/2017/f/ff/Cgu_hardware_baseplate.png" width=70%">
 +
<h2>LightSource</h2>
 +
<p style="font-size:150%">LED light strips with great uniformity of clean white light source provides a clear view of photographing, followed up by analyzing ink spots.
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2017/e/ec/Cgu_hardware_lightSource.png" width="70%">
 +
<h2>Smart Phone</h2>
 +
<p style="font-size:150%">The phone is placed on the top of the device, with the lense targeting the hole for photographing. After the paper is photographed, the picture will be sent to the computer for analyzing. </p>
 +
<h2>Yeast Spray Bottle</h2>
 +
<p style="font-size:150%">Our biological engineered yeast will be filled in the pressure watering bottle which is acid and alkali-resistant. The spray nozzle connected with a stainless steel water pipe is installed on the top cover of the device.</p>
 +
<h2>LED Matrix</h2>
 +
<p style="font-size:150%"> </p>
 +
<img src="https://static.igem.org/mediawiki/2017/9/97/Cgu_hardware_ledMatrix.png" width="70%">
 +
<h2>Circuits, software</h2>
 +
<h3>On the computer</h3>
 +
<p style="font-size:150%">Our image process program using OpenCV function library to decode the JPG image file and do the edge offset, then decide which area have ink. There are two rules for which LED have to be light up. If the pixel which RGB value lower than the general color threshold over a specific percentage of an unit area, or the pixel which RGB value lower than the extreme color threshold over a specific small percentage of an unit area, then the correspond LED will be light up.
 +
</p>
 +
 
 +
<h3>Arduino and circuits</h3>
 +
<p style="font-size:150%">We used Arduino to control the LED matrix in this device. Arduino collect the serial data from the usb port, and send it to the Max7219 microcontroller to light up LED matrix.
 +
 
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2017/d/df/Cgu_hardware_circuit_p3.png" width="70%>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2017/7/7a/CguC4.png" width="20%>
 +
<br>
 +
 
 +
 
  
  
 
</div>
 
</div>
 +
<h3></h3>
 +
<br><br>
 
<!-- Project Description end -->
 
<!-- Project Description end -->
−
 
+
<div>
 +
<br>
 +
</div>
 
<!-- footer start -->
 
<!-- footer start -->
−
<footer align="right">   
+
<footer align="center">   
 
<ul>
 
<ul>
−
<li><a href="http://www.cgu.edu.tw"><img src="https://static.igem.org/mediawiki/2017/1/13/Cgucopyright0915.png" width="40%"></a></li>
+
<li><a href="http://www.cgu.edu.tw"><img src="https://static.igem.org/mediawiki/2017/d/d6/Cguwikifooter.png" width="100%"></a></li>
 
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Latest revision as of 03:59, 16 December 2017

iGem CGU_Taiwan 2017 - Hardware

Hardware


It is localizing the reaction of enzyme on the paper that can reach two goals, reducing the pollution from deinking and maintaining the fibers without inks to keep the strength of the reprocessed paper. Thus, we have created a new device to reform the procedure to make reprocessed paper. Briefly describing the function of the device, the paper will be drew into the box and taken a picture to be analyzed the distribution of the surface area of ink. Then, the engineered yeast will be sprayed on the ink part on the paper to be induced to secrete out enzyme only on the ink part. Thus, the enzyme can be precisely working on the ink part of the paper to preserve the other fibers without the ink on the paper. Additionally, our concept has been confirmed by the pro cedure we made reprocessed paper in lab. We believe our enzymatic deinking process can fulfill our dream with this innovative device.

Structure

The structure of the main part is made of acrylic sheets which are joined with chloroform. Since chloroform welds plastic, it gives the device well stability. For the LED lights, circuit boards are stablized on acrylic sheet drilled.

Component Description

In the following paragraphs, our device will be dismantled into single components and their functions will be explained respectively.

Case

Our device is like an opaque box with a cover on the top and two drawers. Opaque box with internal dimension about 18 cm in width, 20 cm in length and 19 cm in height offers a dim environment which avoids the yeast being induced out of control.


Baseplate

It is located at the bottom of the device. Paper will be placed on it and sent into the case to be analyzed and deinked. The length and width of the plate are both the same as the LED matrix, so paper that is not oversized can apply to the machine.

LightSource

LED light strips with great uniformity of clean white light source provides a clear view of photographing, followed up by analyzing ink spots.

Smart Phone

The phone is placed on the top of the device, with the lense targeting the hole for photographing. After the paper is photographed, the picture will be sent to the computer for analyzing.

Yeast Spray Bottle

Our biological engineered yeast will be filled in the pressure watering bottle which is acid and alkali-resistant. The spray nozzle connected with a stainless steel water pipe is installed on the top cover of the device.

LED Matrix

Circuits, software

On the computer

Our image process program using OpenCV function library to decode the JPG image file and do the edge offset, then decide which area have ink. There are two rules for which LED have to be light up. If the pixel which RGB value lower than the general color threshold over a specific percentage of an unit area, or the pixel which RGB value lower than the extreme color threshold over a specific small percentage of an unit area, then the correspond LED will be light up.

Arduino and circuits

We used Arduino to control the LED matrix in this device. Arduino collect the serial data from the usb port, and send it to the Max7219 microcontroller to light up LED matrix.