Difference between revisions of "Team:ETH Zurich"

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{{ETH_Zurich/Header_N}}
 
{{ETH_Zurich/Header_N}}
 
<html>
 
<html>
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<main role="main">
+
 
−
<figure class="fig-nonfloat">
+
 
−
    <img alt="CATE"
+
<main>
−
     src="https://static.igem.org/mediawiki/2017/c/c3/T--ETH_Zurich--Banner.png"/>
+
 
 +
<div class="banner">
 +
<figure class="banner" id="banner">
 +
     <img src="https://static.igem.org/mediawiki/2017/c/c3/T--ETH_Zurich--Banner.png" alt="CATE" />
 
</figure>
 
</figure>
  
−
<a href="#start">
+
 
−
    <img class="scroll-ico" src="https://static.igem.org/mediawiki/2017/1/14/T--ETH_Zurich--Scroll.png">
+
<figure class="scroll">
 +
<a class="scroll-down-button" href="#scrollstart">
 +
    <img src="https://static.igem.org/mediawiki/2017/1/14/T--ETH_Zurich--Scroll.png">
 
</a>
 
</a>
 +
<figure>
 +
</div>
 +
 +
<section id="scrollstart"></section>
  
−
<!-- TODO: Find better names for the 'Learn More' hyperlinks -->
 
 
<section id="start" class="step">
 
<section id="start" class="step">
−
     <h1>Why?</h1>
+
    <div class="why">
−
    <div>
+
     <h1>WHY</h1>
−
         <p>Cancer kills over 8 million people every year. That's the entire population of Switzerland!</p>
+
         <p>Cancer kills over 8 million people every year. That's as much as the entire population of Switzerland!</p>
−
         <p>We need more specific therapies because current approaches result in many side-effects.</p>
+
         <p>We need more specific therapies because current approaches result in many side-effects. That's why we invented <span title="Came close to being FUSBa (<yyyeaah no ;))">CATE</span>, the first all-in-one living cancer therapeutic with an integrated two-step safety mechanism.
−
        <p>Thats why we invented CATE, the first all-in-one living cancer therapeutic with an integrated two-step safety mechanism.
+
−
        <!-- NOTE: I think this paragraph is a bit cheesy. -->
+
 
         <p>A living cure to a living disease!</p>
 
         <p>A living cure to a living disease!</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Description" class="more"> BACKGROUND</a></p>
 
     </div>
 
     </div>
−
    <img src="https://static.igem.org/mediawiki/2017/9/99/T--ETH_Zurich--CH.png" class="CH">
 
−
    <a href="/Team:ETH_Zurich/Background" class="immutable more">Learn more</a>
 
 
</section>
 
</section>
  
−
<section id="second" class="step invert">
+
 
−
     <h1>Vision</h1>
+
 
−
    <div>
+
<section id="second" class="step">
−
         <p>CATE consists of the non-pathogenic bacterium  <span class="bacterium">E. coli</span> Nissle that has the intrinsic ability to home preferentially in tumors.</p>
+
     <div class="vision">
−
        <p>We are engineering <span class="bacterium">E. coli</span> Nissle to carry a MRI contrast and a cytotoxic agent so it can deliver both components to tumor sites.</p>
+
<figure class="EcN">
−
         <p>CATE consists of the non-pathogenic bacterium E. coli Nissle that has the intrinsic ability to home specifically in tumors. It features two safety checkpoint mechanisms to ensure only tumor cells are damaged.</p>
+
         <img src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--Ec.png">
 +
</figure>
 +
<br>
 +
<br>
 +
    <h1>VISION</h1>
 +
        <p>To tackle the challenge of treating cancer, we decided to look beyond classical approaches and from the point of view of a synthetic biologist. </p>
 +
         <p>Our search led us to the concept of bacterial cancer therapy.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Description" class="more">STORY OF CATE</a></p>
 
     </div>
 
     </div>
−
    <img src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--Ec.png" class="Ec">
+
 
−
    <a href="/Team:ETH_Zurich/Description" class="immutable more">Learn more</a>
+
 
</section>
 
</section>
 +
 +
 +
 +
<div class="space">
 +
  &nbsp;
 +
</div>
 +
  
 
<section id="third" class="step">
 
<section id="third" class="step">
−
    <h1>Treatment</h1>
 
 
     <div>
 
     <div>
−
         <p>CATE is administered intravenously, travels through the blood and colonizes tumors where the bacteria form a highly dense layer between the live and dead zone of the tumor</p>
+
         <p>CATE consists of the non-pathogenic bacterium <span class="bacterium">E. coli</span> Nissle that has the intrinsic ability to home preferentially in tumors.</p>
−
         <p>The high density of bacterial cells and the overproduction of lactate by the tumor together activate the first steps of CATE.</p>
+
         <p>It features two safety checkpoint mechanisms to ensure only tumor cells are damaged.</p>
−
        <!-- TODO: Merge with the following.
+
         <p>CATE is administered intravenously, travels through the blood and colonizes tumors. When enough bacteria have accumulated in the tumor, they make themselves visible and  
−
         <p>CATE is administered intravenously, travels through the blood and colonizes tumors. If enough bacteria have gathered at the tumor, they make themselves visible and prepare a payload. A doctor can then control the site with an MRI and activate release of the cancer-killing payload.
+
      start preparing the cytotoxic payload.</p>
−
         -->
+
        <p> After imaging the tumor with MRI, the doctor can then activate the release of the cancer-killing payload. </p>
 +
         <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Applied_Design" class="more">CATE IN ACTION</a></p>
 
     </div>
 
     </div>
−
    <img src="https://static.igem.org/mediawiki/2017/8/8c/T--ETH_Zurich--ANDgate.png" class="AND">
+
 
−
    <!-- TODO: Fix the following link -->
+
 
−
    <a href="/Team:ETH_Zurich/#" class="immutable more">Learn More</a>
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<figure class="process">
−
    <!-- NOTE: Consider
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  <img src="https://static.igem.org/mediawiki/2017/c/cf/T--ETH_Zurich--process.png">
−
    <a href="/Team:ETH_Zurich/Applied_Design" class="immutable more">Treatment</a>
+
</figure>
−
    -->
+
 
</section>
 
</section>
  
−
<section>
+
 
−
    <h1>Circuit</h1>
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 +
<div class="space">
 +
  &nbsp;
 +
</div>
 +
 
 +
<section id="fourth" class="step">
 +
<div class="circuit">
 +
<br>
 +
<br>
 +
<br>
 +
<figure class="andgate">
 +
  <img src="https://static.igem.org/mediawiki/2017/8/8c/T--ETH_Zurich--ANDgate.png">
 +
</figure>
 
     <div>
 
     <div>
−
         <p>To achieve all these novel functions, we designed a genetic circuit that is distributed over two de novo synthesized DNA molecules. All functions were tested and optimized to make the resulting circuit as safe and well characterized as possible.</p>
+
 
 +
         <p>To achieve all these novel functions, we designed a genetic circuit that is distributed over two synthetic DNA sequences.</p>
 +
        <p> All functions were tested and optimized to make the resulting circuit as safe and well-characterized as possible.</p>
 +
        <br>
 +
      <p><a href="https://2017.igem.org/Team:ETH_Zurich/Circuit" class="more">CIRCUIT</a></p>
 
     </div>
 
     </div>
−
    <a href="/Team:ETH_Zurich/Circuit" class="immutable more">Learn More</a>
 
 
</section>
 
</section>
 +
 +
 +
<section id="fifth" class="step">
 +
<div class="drylab">
 +
<figure class="drylab">
 +
  <img src="https://static.igem.org/mediawiki/2017/0/0b/T--ETH_Zurich--dry_lab.png">
 +
</figure>
 +
<br>
 +
<h1>ENGINEERING</h1>
 +
        <p>We increased the understanding of the system's underlying mathematics by simulating its behavior with our models.</p><p> The models were then used to define important questions to clarify in experiments and develop efficient experimental and genetic design strategies.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Model" class="more">DRY LAB</a></p>
 +
  </div>
 +
</section>
 +
  
 
<section>
 
<section>
−
     <h1>Engineering</h1>
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     <div class="wetlab">
−
    <div>
+
<figure class="wetlab">
−
        <p>We increased the understanding of the systems underlying mathematics by simulating the functions with models. The models were also used to define important questions to clarify in experiments.</p>
+
  <img src="https://static.igem.org/mediawiki/2017/9/95/T--ETH_Zurich--wetlab.png">
 +
</figure>
 +
<br>
 
         <p>Experimentally, we collected data to support and refine our models and to show that our system works.</p>
 
         <p>Experimentally, we collected data to support and refine our models and to show that our system works.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Experiments" class="more">WET LAB</a></p>
 
     </div>
 
     </div>
−
    <!-- NOTE: We need a /Dry_Lab maybe
 
−
    <a href="/Team:ETH_Zurich/Applied_Design" class="immutable more">Treatment</a>
 
−
    -->
 
−
    <a href="/Team:ETH_Zurich/Engineering" class="immutable more">Learn More</a>
 
 
</section>
 
</section>
 +
  
 
<section>
 
<section>
−
     <div>
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     <div class="goals">
−
         <p>We worked goal oriented and could experimentally confirm the predictions of the models. After testing every function individually, we combined them one after the other in milestone experiments to show the system in action. We created and characterized new BioBrick parts that are important for the iGEM competition and are freely for the future iGEM teams.</p>
+
<figure class="goals">
 +
    <img src="https://static.igem.org/mediawiki/2017/8/83/T--ETH_Zurich--achievementslanding.png">
 +
</figure>
 +
    <h1>ACHIEVEMENTS</h1>
 +
         <p>We could experimentally confirm the predictions of the models. After testing every function individually, we combined them one after the other in milestone experiments to show the system in action.</p> <p> We created and characterized new BioBrick parts as a contribution for the synthetic biology community.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Results" class="more">ACHIEVEMENTS</a></p>
 
     </div>
 
     </div>
−
    <a href="/Team:ETH_Zurich/Achievements" class="immutable more">Learn More</a>
 
 
</section>
 
</section>
  
−
<!-- TODO: Add a section about Human Practices -->
 
  
 
<section>
 
<section>
−
     <h1>Team</h1>
+
  <div class="HP">
−
     <div>
+
     <h1>HUMAN PRACTICES</h1>
 +
<figure class="HP">
 +
     <img src="https://static.igem.org/mediawiki/2017/4/4a/T--ETH_Zurich--HPlanding.png">
 +
</figure>
 +
        <p>We went beyond the lab and reached out to experts to better understand current technological and safety issues in order to enhance the design of our project.</p><p> Further, we         
 +
        introduced our project and the field of synthetic biology to the general public and together explored issues related to safety, ethics and sustainability.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Human_Practices" class="more">HUMAN PRACTICES</a></p>
 +
    </div>
 +
</section>
 +
 
 +
 
 +
<section>
 +
    <div class="team">
 +
<figure class="team">
 +
    <img src="https://static.igem.org/mediawiki/2017/e/ec/T--ETH_Zurich--teamlanding.png">
 +
</figure>
 +
    <h1>TEAM</h1>
 
         <p>We are an interdisciplinary team of eight master students of ETH Zürich who compete in the iGEM championship against hundreds of other teams from all over the world.</p>
 
         <p>We are an interdisciplinary team of eight master students of ETH Zürich who compete in the iGEM championship against hundreds of other teams from all over the world.</p>
 +
        <br>
 +
        <p><a href="https://2017.igem.org/Team:ETH_Zurich/Team" class="more">TEAM</a></p>
 
     </div>
 
     </div>
−
    <a href="/Team:ETH_Zurich/Team" class="immutable more">Learn More</a>
 
 
</section>
 
</section>
 +
  
 
</main>
 
</main>
 +
 
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<script>
 
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             $('html, body').animate({ scrollTop: $($(this).attr('href')).offset().top}, 500, 'linear');
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             $('html, body').animate({ scrollTop: $($(this).attr('href')).offset().top}, 600, 'linear');
 
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</html>
 
</html>
 
{{ETH_Zurich/Footer_N}}
 
{{ETH_Zurich/Footer_N}}

Latest revision as of 14:06, 14 December 2017

WHY

Cancer kills over 8 million people every year. That's as much as the entire population of Switzerland!

We need more specific therapies because current approaches result in many side-effects. That's why we invented CATE, the first all-in-one living cancer therapeutic with an integrated two-step safety mechanism.

A living cure to a living disease!


BACKGROUND



VISION

To tackle the challenge of treating cancer, we decided to look beyond classical approaches and from the point of view of a synthetic biologist.

Our search led us to the concept of bacterial cancer therapy.


STORY OF CATE

 

CATE consists of the non-pathogenic bacterium E. coli Nissle that has the intrinsic ability to home preferentially in tumors.

It features two safety checkpoint mechanisms to ensure only tumor cells are damaged.

CATE is administered intravenously, travels through the blood and colonizes tumors. When enough bacteria have accumulated in the tumor, they make themselves visible and start preparing the cytotoxic payload.

After imaging the tumor with MRI, the doctor can then activate the release of the cancer-killing payload.


CATE IN ACTION

 



To achieve all these novel functions, we designed a genetic circuit that is distributed over two synthetic DNA sequences.

All functions were tested and optimized to make the resulting circuit as safe and well-characterized as possible.


CIRCUIT


ENGINEERING

We increased the understanding of the system's underlying mathematics by simulating its behavior with our models.

The models were then used to define important questions to clarify in experiments and develop efficient experimental and genetic design strategies.


DRY LAB


Experimentally, we collected data to support and refine our models and to show that our system works.


WET LAB

ACHIEVEMENTS

We could experimentally confirm the predictions of the models. After testing every function individually, we combined them one after the other in milestone experiments to show the system in action.

We created and characterized new BioBrick parts as a contribution for the synthetic biology community.


ACHIEVEMENTS

HUMAN PRACTICES

We went beyond the lab and reached out to experts to better understand current technological and safety issues in order to enhance the design of our project.

Further, we introduced our project and the field of synthetic biology to the general public and together explored issues related to safety, ethics and sustainability.


HUMAN PRACTICES

TEAM

We are an interdisciplinary team of eight master students of ETH Zürich who compete in the iGEM championship against hundreds of other teams from all over the world.


TEAM