Line 1,152: | Line 1,152: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I> </td> |
<td> pBAD30(EcoR I and Hind III cut)</td> | <td> pBAD30(EcoR I and Hind III cut)</td> | ||
<td>Top10 </td> | <td>Top10 </td> | ||
Line 1,178: | Line 1,178: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pBAD30</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pBAD30</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>6</td> | <td>6</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pBAD30</td> |
</tr> | </tr> | ||
Line 1,603: | Line 1,603: | ||
</div> | </div> | ||
− | <!--*****<I>PmrA-PmrB(LBTX)</I>-pBAD30→<I>PmrA-PmrB(LBT X)</I>- | + | <!--*****<I>PmrA-PmrB(LBTX)</I>-pBAD30→<I>PmrA-PmrB(LBT X)</I>-PmrC-GFP-pBAD30*****PAGE******--> |
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
− | <h4><strong><I>PmrA-PmrB(LBT X)</I>-pBAD30→<I>PmrA-PmrB(LBT X)- | + | <h4><strong><I>PmrA-PmrB(LBT X)</I>-pBAD30→<I>PmrA-PmrB(LBT X)-PmrC-GFP</I>-pBAD30</strong> </h4> |
<p><strong>10.18</strong></p> | <p><strong>10.18</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
Line 1,785: | Line 1,785: | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Standardization Part</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Standardization Part</h3> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
− | <h4><strong><I> | + | <h4><strong><I>PmrA-PmrB-LBT X</I>-pBAD30→<I>PmrA-PmrB-LBT X-PmrC-GFP</I>-pBAD30</strong> </h4> |
<p><strong>8.20</strong></p> | <p><strong>8.20</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
Line 2,139: | Line 2,139: | ||
</div> | </div> | ||
− | <!--*********<I> | + | <!--*********<I>PmrC-GFP</I>-pBAD30→<I>PmrC-GFP</I>-pSB1C3**************Page21****************--> |
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
− | <h4><strong><I> | + | <h4><strong><I>PmrC-GFP</I>-pBAD30→<I>PmrC-GFP</I>-pSB1C3</strong> </h4> |
<p><strong>10.2</strong></p> | <p><strong>10.2</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
Line 2,155: | Line 2,155: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I></td> |
<td>30 μl</td> | <td>30 μl</td> | ||
</tr> | </tr> | ||
Line 2,177: | Line 2,177: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I></td> |
<td>Pstl I and XbaI</td> | <td>Pstl I and XbaI</td> | ||
</tr> | </tr> | ||
Line 2,202: | Line 2,202: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I></td> |
<td>pSB1C3(Pstl I and XbaI cut)</td> | <td>pSB1C3(Pstl I and XbaI cut)</td> | ||
<td>DH5a</td> | <td>DH5a</td> | ||
Line 2,227: | Line 2,227: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>3</td> | <td>3</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
− | <td><I> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
Line 2,252: | Line 2,252: | ||
<!--****************Page 22****************************--> | <!--****************Page 22****************************--> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
− | <h4><strong><I> | + | <h4><strong><I>PmrA-PmrB-LBT X-PmrC-GFP</I>-pBAD30→<I>PmrA-PmrB-LBT X-PmrC-GFP</I>-pSB1C3</strong> </h4> |
<p><strong>10.26</strong></p> | <p><strong>10.26</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
Line 2,266: | Line 2,266: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 5-PmrC-GFP</I></td> |
<td>30 μl</td> | <td>30 μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 9-PmrC-GFP</I></td> |
<td>30 μl</td> | <td>30 μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 11-PmrC-GFP</I></td> |
<td>30 μl</td> | <td>30 μl</td> | ||
</tr> | </tr> | ||
Line 2,296: | Line 2,296: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 5-PmrC-GFP</I></td> |
<td>Pst I and XbaI</td> | <td>Pst I and XbaI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 9-PmrC-GFP</I></td> |
<td>Pst I and XbaI</td> | <td>Pst I and XbaI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 11-PmrC-GFP</I></td> |
<td>Pst I and XbaI</td> | <td>Pst I and XbaI</td> | ||
</tr> | </tr> | ||
Line 2,329: | Line 2,329: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 5-PmrC-GFP</I></td> |
<td>pSB1C3(Pst I and Spel I cut)</td> | <td>pSB1C3(Pst I and Spel I cut)</td> | ||
<td>DH5a</td> | <td>DH5a</td> | ||
Line 2,335: | Line 2,335: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 9-PmrC-GFP</I></td> |
<td>pSB1C3(Pst I and Spel I cut)</td> | <td>pSB1C3(Pst I and Spel I cut)</td> | ||
<td>DH5a</td> | <td>DH5a</td> | ||
Line 2,341: | Line 2,341: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 11-PmrC-GFP</I></td> |
<td>pSB1C3(Pst I and Spel I cut)</td> | <td>pSB1C3(Pst I and Spel I cut)</td> | ||
<td>DH5a</td> | <td>DH5a</td> | ||
Line 2,366: | Line 2,366: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 5-PmrC-GFP</I></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 9-PmrC-GFP</I></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>3</td> | <td>3</td> | ||
− | <td><I> | + | <td><I>PmrA-PmrB-LBT 11-PmrC-GFP</I></td> |
</tr> | </tr> | ||
</tbody> | </tbody> |
Revision as of 10:40, 1 November 2017