Difference between revisions of "Team:HUST-China/Notebook"
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body { | body { | ||
| − | + | position: relative; | |
| − | + | ||
| − | + | ||
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| − | + | ||
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} | } | ||
| + | @media (min-width: 768px) { | ||
| + | .menu_logo_1 { | ||
| + | top: 18px; | ||
| + | left:10px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .menu_logo_2 { | ||
| + | top: 18px; | ||
| + | left:100px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .nav-pills{ | ||
| + | font-size:8px; | ||
| + | font-weight: bold; | ||
| + | } | ||
| + | } | ||
| + | |||
| + | /* 中等屏幕（桌面显示器，大于等于992px） */ | ||
| + | @media (min-width: 992px) { | ||
| + | .menu_logo_1 { | ||
| + | top: 18px; | ||
| + | left:20px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .menu_logo_2 { | ||
| + | top: 18px; | ||
| + | left:110px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .nav-pills{ | ||
| + | font-size:10px; | ||
| + | font-weight: bold; | ||
| + | } | ||
| + | } | ||
| + | |||
| + | /* 大屏幕（大桌面显示器，大于等于1200px） */ | ||
| + | @media (min-width: 1200px) { | ||
| + | .menu_logo_1 { | ||
| + | top: 18px; | ||
| + | left:80px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .menu_logo_2 { | ||
| + | top: 18px; | ||
| + | left:170px; | ||
| + | z-index: 1000; | ||
| + | position: fixed; | ||
| + | } | ||
| + | .nav-pills{ | ||
| + | font-size:12px; | ||
| + | font-weight: bold; | ||
| + | } | ||
| + | |||
| + | } | ||
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<ul class="nav nav-pills nav-stacked" style="font-size:12px; text-align:center; background:#ccc;"> | <ul class="nav nav-pills nav-stacked" style="font-size:12px; text-align:center; background:#ccc;"> | ||
<li class="active"><a href="#section1">Sensing part</a></li> | <li class="active"><a href="#section1">Sensing part</a></li> | ||
| − | <li><a href="#section2"> | + | <li><a href="#section2">Capture part</a></li> |
<li><a href="#section3">Standardization Part</a></li> | <li><a href="#section3">Standardization Part</a></li> | ||
</ul> | </ul> | ||
| Line 467: | Line 512: | ||
</nav> | </nav> | ||
<div class="container col-sm-8"> | <div class="container col-sm-8"> | ||
| − | <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | + | |
| − | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Sensing part</h3> | + | |
| − | <h4><strong> | + | |
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
| + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Sensing part</h3> | ||
| + | <h4><strong><I>PmrC-GFP</I>→<I>PmrC-GFP</I>-pBAD30</strong> </h4> | ||
| + | |||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.10</strong></p> | ||
| + | <p><strong>PCR</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>PmrC-GFP</td> | ||
| + | <td>50μl</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/a/a6/2017_HUST_China_notebook_img6.png" alt="demo1" class="col-xs-6 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.19</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>pBAD30</td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.20</strong></p> | ||
| + | <p><strong>DNA ligation & transformation</strong></p> | ||
| + | <p><strong>Yiyan Yu & Qingyang Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrC-GFP</I> </td> | ||
| + | <td> pBAD30(EcoR I and Hind III cut)</td> | ||
| + | <td>Top10 </td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.21</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrC-GFP</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrC-GFP</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrC-GFP</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <h4><strong><I>PmrA-PmrB(Fe)</I>→<I>PmrA-PmrB(Fe)</I>-pBAD30</strong> </h4> | ||
| + | <p><strong>7.8</strong></p> | ||
| + | <p><strong>PCR</strong></p> | ||
| + | <p><strong>Long Cheng</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(Fe)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | |||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b5/2017_HUST_China_notebook_img7.png" alt="demo1" class="col-xs-6 col-xs-offset-2"> | ||
| + | </div> | ||
| + | <!--**Page10********************************************************--> | ||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.19</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(Fe)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>pBAD30</td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.20</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Yiyan Yu & Qingyang Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(Fe)</I></td> | ||
| + | <td> pBAD30(EcoR I and Hind III cut)</td> | ||
| + | <td>Top10 </td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>7.21</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(Fe)</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrA-PmrB(Fe)</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(Fe)</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/a/ac/2017_HUST_China_notebook_img8.png" alt="demo1" class="col-xs-6 col-xs-offset-2"> | ||
| + | |||
| + | </div> | ||
| + | <!--**Page11********************************************************--> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <h4><strong><I>PmrA-PmrB(Fe)</I>-pBAD30→<I>PmrA-PmrB(LBTX)</I>-pBAD30</strong> </h4> | ||
| + | <p><strong>7.25</strong></p> | ||
| + | <p><strong>PCR</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>LBT</I> 1 to 12</td> | ||
| + | <td>50 μl each</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/e/e5/2017_HUST_China_notebook_img9.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.10</strong></p> | ||
| + | <p><strong>Point mutation</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(Fe)</I>-pBAD30</td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.20</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(Fe)</I>-pBAD30</td> | ||
| + | <td>AhdI and SacI</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>LBT 1</I></td> | ||
| + | <td>AhdI and SacI</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>LBT 11</I></td> | ||
| + | <td>AhdI and SacI</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | |||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/8/87/2017_HUST_China_notebook_img10.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.20</strong></p> | ||
| + | <p><strong>Transformation</strong></p> | ||
| + | <p><strong>Xiaoyang Li & Yating Hu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>LBT 1</td> | ||
| + | <td> <I>PmrA-PmrB(Fe)</I>-pBAD30(AhdI and SacI cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>LBT 11</td> | ||
| + | <td> <I>PmrA-PmrB(Fe)</I>-pBAD30(AhdI and SacI cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.21</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 1)</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)</I>-pBAD30</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/d/d6/2017_HUST_China_notebook_img11.png" alt="demo1" class="col-xs-6 col-xs-offset-2"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.31</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT2)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT3)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT4)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT5)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT6)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT7)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT8)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT9)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT10)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT12)</I></td> | ||
| + | <td>EcoRI and KpnI</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | |||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/6/6d/2017_HUST_China_notebook_img12.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>8.31</strong></p> | ||
| + | <p><strong>Transformation</strong></p> | ||
| + | <p><strong>Xiaoyang Li & Yating Hu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 2,3,4,5,6,7,8,9,10,12)</I></td> | ||
| + | <td>pBAD30(EcoR I and Kpn I cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>9.3</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT2)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT3)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT4)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT5)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrA-PmrB(LBT6)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(LBT7)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>7</td> | ||
| + | <td><I>PmrA-PmrB(LBT8)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>8</td> | ||
| + | <td><I>PmrA-PmrB(LBT9)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>9</td> | ||
| + | <td><I>PmrA-PmrB(LBT10)</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>10</td> | ||
| + | <td><I>PmrA-PmrB(LBT12)</I></td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/3/3f/2017_HUST_China_notebook_img13.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | <!--*****<I>PmrA-PmrB(LBTX)</I>-pBAD30→<I>PmrA-PmrB(LBT X)</I>-PmrC-GFP-pBAD30*****PAGE******--> | ||
| + | <div class="col-xs-12"> | ||
| + | <h4><strong><I>PmrA-PmrB(LBT X)</I>-pBAD30→<I>PmrA-PmrB(LBT X)-PmrC-GFP</I>-pBAD30</strong> </h4> | ||
| + | <p><strong>10.18</strong></p> | ||
| + | <p><strong>PCR</strong></p> | ||
| + | <p><strong>Long Cheng</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)</I></td> | ||
| + | <td>30 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)</I></td> | ||
| + | <td>30 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)</I></td> | ||
| + | <td>30 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.19</strong></p> | ||
| + | <p><strong>SOE PCR</strong></p> | ||
| + | <p><strong>Xiaoyang Li & Qingyang Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)</I> & PmrC-GFP</td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)</I> & PmrC-GFP</td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)</I> & PmrC-GFP</td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/2/25/2017_HUST_China_notebook_img14.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.19</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)</I> & <I>PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)</I> & <I>PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)</I> & <I>PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>pBAD30</td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.19</strong></p> | ||
| + | <p><strong>DNA ligation & transformation</strong></p> | ||
| + | <p><strong>Xiaoyang Li & Yating Hu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.20</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.19</strong></p> | ||
| + | <p><strong>SOE PCR</strong></p> | ||
| + | <p><strong> Qingyang Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 3)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 6)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 7)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 10)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/9/9d/2017_HUST_China_notebook_pic7.jpg" class="col-xs-8 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.24</strong></p> | ||
| + | <p><strong>SOE PCR</strong></p> | ||
| + | <p><strong> Qingyang Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT3)-PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT6)-PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 7)-PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 10)-PmrC-GFP</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/a/ad/2017_HUST_China_notebook_pic8.jpg" class="col-xs-6 col-xs-offset-2"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.19</strong></p> | ||
| + | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 3)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 6)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 7)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 10)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>pBAD30</td> | ||
| + | <td>EcoRI and Hind III</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/4/4c/2017_HUST_China_notebook_pic9.jpg" class="col-xs-8 col-xs-offset-2"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.26</strong></p> | ||
| + | <p><strong>DNA ligation & transformation</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Backbone</th> | ||
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 3)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 6)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 7)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 10)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | <td>pBAD30(EcoRI and Hind III cut)</td> | ||
| + | <td>Top10</td> | ||
| + | <td>LB(amp added)</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-12" > | ||
| + | <p><strong>10.28</strong></p> | ||
| + | <p><strong>Colony PCR</strong></p> | ||
| + | <p><strong>Xiaoyang Li</strong></p> | ||
| + | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| + | <table class="table table-hover"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
| + | <th style="text-align: center;">Name</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(LBT 5)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 6)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT 6)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT 7)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 7)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 10)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT 10)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | <tr> | ||
| + | <td>7</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>8</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>9</td> | ||
| + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/0/07/2017_HUST_China_notebook_pic10.jpg" class="col-xs-8 col-xs-offset-2" | ||
| + | style="padding:10px;"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div id="section2" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
| + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Capture part</h3> | ||
| + | <h4><strong><I>oprF</I>→<I>oprF</I>-pET28a</strong> </h4> | ||
<p><strong>7.2</strong></p> | <p><strong>7.2</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
| Line 484: | Line 1,615: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 1</td> |
<td>50 μl</td> | <td>50 μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 2</td> |
<td>50 μl</td> | <td>50 μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 3</td> |
<td>50 μl</td> | <td>50 μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 4</td> |
<td>50 μl</td> | <td>50 μl</td> | ||
</tr> | </tr> | ||
| Line 523: | Line 1,654: | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 1</td> |
<td>NheI and BamHI</td> | <td>NheI and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I> 2</td> |
<td>NheI and BamHI</td> | <td>NheI and BamHI</td> | ||
</tr> | </tr> | ||
| Line 551: | Line 1,682: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td> <I>oprF</I> </td> |
<td>pET28a(BamH I and Nhe I cut)</td> | <td>pET28a(BamH I and Nhe I cut)</td> | ||
<td> Top10</td> | <td> Top10</td> | ||
| Line 576: | Line 1,707: | ||
<tr> | <tr> | ||
<td> 1 </td> | <td> 1 </td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> 2 </td> | <td> 2 </td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> 3 </td> | <td> 3 </td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> 4 </td> | <td> 4 </td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> 5 </td> | <td> 5 </td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
| Line 600: | Line 1,731: | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <h4><strong>3×LBT1-12 & | + | <h4><strong><I>3×LBT1-12</I> & <I>Sitag</I>→<I>oprF-3×LBT</I> X(or <I>Sitag</I>)-pET28a</strong></h4> |
<p><strong>7.22</strong></p> | <p><strong>7.22</strong></p> | ||
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
| Line 608: | Line 1,739: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Total</th> | <th style="text-align: center;">Total</th> | ||
</tr> | </tr> | ||
| Line 614: | Line 1,745: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT1</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT2</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT3</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT4</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT9</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT10</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT11</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT12</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
| Line 658: | Line 1,789: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Total</th> | <th style="text-align: center;">Total</th> | ||
</tr> | </tr> | ||
| Line 664: | Line 1,795: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT5_1</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT5_2</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT6_1</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT6_2</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT7_1</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT7_2</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT8_1</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT8_2</I></td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Sitag 1</td> | + | <td><I>Sitag</I> 1</td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>Sitag</I> 2</td> |
<td>50μl</td> | <td>50μl</td> | ||
</tr> | </tr> | ||
| Line 718: | Line 1,849: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Enzyme</th> | <th style="text-align: center;">Enzyme</th> | ||
</tr> | </tr> | ||
| Line 724: | Line 1,855: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT2</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT3</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT4</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT8</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>Sitag</I></td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
| Line 802: | Line 1,891: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Enzyme</th> | <th style="text-align: center;">Enzyme</th> | ||
</tr> | </tr> | ||
| Line 808: | Line 1,897: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT1</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT5</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT6</I></td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT7</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT9</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT10</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT11</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT12</td> |
<td>HindIII and BamHI</td> | <td>HindIII and BamHI</td> | ||
</tr> | </tr> | ||
| Line 857: | Line 1,946: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Backbone</th> | <th style="text-align: center;">Backbone</th> | ||
<th style="text-align: center;">Stain</th> | <th style="text-align: center;">Stain</th> | ||
| Line 865: | Line 1,954: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT2</td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT3</td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT4</td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td>3 x LBT8</td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
| Line 890: | Line 1,979: | ||
<tr> | <tr> | ||
<td>Sitag</td> | <td>Sitag</td> | ||
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
| Line 916: | Line 2,005: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
| − | <td> | + | <td><I>oprF</I>-3×LBT2-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
| − | <td> | + | <td><I>oprF</I>-3×LBT3-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>3</td> | <td>3</td> | ||
| − | <td> | + | <td><I>oprF</I>-3×LBT4-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
| − | <td> | + | <td><I>oprF</I>-3×LBT8-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
| − | <td> | + | <td><I>oprF</I>-Sitag-pET28a</td> |
</tr> | </tr> | ||
| Line 950: | Line 2,039: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Name</th> |
<th style="text-align: center;">Backbone</th> | <th style="text-align: center;">Backbone</th> | ||
<th style="text-align: center;">Stain</th> | <th style="text-align: center;">Stain</th> | ||
| Line 958: | Line 2,047: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT1</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT5</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT6</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT7</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
| Line 983: | Line 2,072: | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT9</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT10</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT11</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>3 | + | <td><I>3 x LBT12</I></td> |
| − | <td> | + | <td><I>oprF</I>-pET28a(BamHI and HindIII cut)</td> |
<td>Top10</td> | <td>Top10</td> | ||
<td>LB(kan added)</td> | <td>LB(kan added)</td> | ||
| Line 1,028: | Line 2,117: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT2</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT3</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>3</td> | <td>3</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT4</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT8</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
| − | <td> | + | <td><I>oprF-Sitag</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>6</td> | <td>6</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT4</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>7</td> | <td>7</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT8</I>-pET28a</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>8</td> | <td>8</td> | ||
| − | <td> | + | <td><I>oprF-Sitag</I>-pET28a</td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
| Line 1,072: | Line 2,161: | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| + | <!--********************Standardization Part********PAGE 17*************--> | ||
| + | <div id="section3" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
| + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Standardization Part</h3> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>8.20</strong></p> |
<p><strong>PCR</strong></p> | <p><strong>PCR</strong></p> | ||
| − | <p><strong> | + | <p><strong>Yubei Sun & Huiping Shi</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| − | + | <table class="table table-hover"> | |
<thead> | <thead> | ||
<tr> | <tr> | ||
| Line 1,094: | Line 2,181: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT1</I></td> |
| − | <td> | + | <td>50 μl</td> |
| − | </tr> | + | </tr> |
| − | + | <tr> | |
| + | <td><I>oprF-3 X LBT4</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT8</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT10</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT11</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT12</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | <img title="demo1" src="https://static.igem.org/mediawiki/2017/ | + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/c/c4/2017_HUST_China_notebook_img15.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> |
</div> | </div> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>9.20</strong></p> |
| − | <p><strong> | + | <p><strong>SOE PCR</strong></p> |
| − | <p><strong> | + | <p><strong>Efan Wang</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| − | + | <table class="table table-hover"> | |
<thead> | <thead> | ||
<tr> | <tr> | ||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Total</th> |
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT3</I></td> |
| − | <td> | + | <td>50 μl</td> |
| − | </tr> | + | </tr> |
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT6</I></td> |
| − | <td> | + | <td>50 μl</td> |
| − | </tr> | + | </tr> |
| − | + | <tr> | |
| + | <td><I>oprF-3 X LBT7</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-Sitag</I></td> | ||
| + | <td>50 μl</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | <img title="demo1" src="https://static.igem.org/mediawiki/2017/5/5c/2017_HUST_China_notebook_img16.png" alt="demo1" class="col-xs-4 col-xs-offset-2"> | ||
</div> | </div> | ||
| − | |||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>9.5</strong></p> |
| − | <p><strong> | + | <p><strong>Restriction Enzyme Digestion</strong></p> |
| − | <p><strong> | + | <p><strong>Yubei Sun & Ziyi Li</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| − | + | <table class="table table-hover"> | |
<thead> | <thead> | ||
<tr> | <tr> | ||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Enzyme</th> |
| − | + | ||
| − | + | ||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 1</I></td> |
| − | <td> | + | <td>EcoRI and Spel I</td> |
| − | <td> | + | </tr> |
| − | <td> | + | |
| − | </tr> | + | <tr> |
| − | + | <td><I>oprF-3 X LBT 4</I></td> | |
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 10</I></td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 11</I></td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>pSB1C3</td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>pSB1C3</td> | ||
| + | <td>Xbal I and Pst I</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | |||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>9.20</strong></p> |
| − | <p><strong> | + | <p><strong>Restriction Enzyme Digestion</strong></p> |
| − | <p><strong> | + | <p><strong>Huiping Shi</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
| − | + | <table class="table table-hover"> | |
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | |||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Enzyme</th> | ||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 3</I></td> |
| − | <td> | + | <td>EcoRI and Spel I</td> |
| − | </tr> | + | </tr> |
| + | |||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 6</I></td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 7</I></td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 8</I></td> | ||
| + | <td>EcoRI and Spel I</td> | ||
| + | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 11</I></td> |
| − | <td> | + | <td>EcoRI and Spel I</td> |
| − | </tr> | + | </tr> |
| + | |||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 12</I></td> |
| − | <td> | + | <td>EcoRI and Spel I</td> |
| − | </tr> | + | </tr> |
| − | + | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | + | <p><strong>9.27</strong></p> | |
| − | <p><strong> | + | <p><strong>DNA ligation & transformation</strong></p> |
| − | <p><strong> | + | <p><strong>Yubei Sun & Huiping Shi</strong></p> |
| − | <p><strong> | + | |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,201: | Line 2,347: | ||
<tr> | <tr> | ||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Backbone</th> |
| + | <th style="text-align: center;">Strain </th> | ||
| + | <th style="text-align: center;">Medium</th> | ||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 1</I></td> |
| − | <td> | + | <td>pSB1C3(EcoRI and Spel I cut)</td> |
| − | </tr> | + | <td>DH5a</td> |
| − | + | <td>LB(chl added)</td> | |
| − | + | </tr> | |
| − | + | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 3</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 4</I></td> |
| − | <td>EcoRI and | + | <td>pSB1C3(EcoRI and Spel I cut)</td> |
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
</tr> | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 8</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-Sitag</I></td> |
| − | <td>EcoRI and | + | <td>pSB1C3(EcoRI and Spel I cut)</td> |
| − | </tr> | + | <td>DH5a</td> |
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,242: | Line 2,391: | ||
</div> | </div> | ||
| − | + | <div class="col-xs-12"> | |
| − | <p><strong> | + | <p><strong>9.27</strong></p> |
| − | <p><strong> | + | <p><strong>DNA ligation & transformation</strong></p> |
| − | <p><strong> | + | <p><strong>Ziyang Xiao</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,258: | Line 2,407: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>oprF-3 X LBT 6</I></td> |
| − | <td> | + | <td>pSB1C3(EcoRI and Spel I cut)</td> |
| − | <td> | + | <td>DH5a</td> |
| − | <td>LB( | + | <td>LB(chl added)</td> |
| − | </tr> | + | </tr> |
| − | + | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 7</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 10</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 11</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>oprF-3 X LBT 12</I></td> | ||
| + | <td>pSB1C3(EcoRI and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,269: | Line 2,444: | ||
</div> | </div> | ||
| − | + | <div class="col-xs-12"> | |
| − | + | <p><strong>9.28</strong></p> | |
| − | <p><strong> | + | |
<p><strong>Colony PCR</strong></p> | <p><strong>Colony PCR</strong></p> | ||
| − | <p><strong> | + | <p><strong>Yubei Sun</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,285: | Line 2,459: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
| − | <td> | + | <td><I>oprF-3×LBT1</I>-pSB1C3</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>oprF-3×LBT3</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>oprF-3×LBT4</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>oprF-3×LBT8</I>-pSB1C3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
| − | <td> | + | <td><I>oprF-Sitag</I>-pSB1C3</td> |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | </tr> | + | |
| − | + | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | + | </div> | |
| − | + | <div class="col-xs-12"> | |
| − | + | <p><strong>9.30</strong></p> | |
| − | + | <p><strong>Colony PCR</strong></p> | |
| − | + | <p><strong>Ziyang Xiao</strong></p> | |
| − | + | ||
| − | <p><strong> | + | |
| − | <p><strong>PCR</strong></p> | + | |
| − | <p><strong> | + | |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th style="text-align: center;">Number</th> | ||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| − | |||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>1</td> |
| − | <td> | + | <td><I>oprF-3×LBT6</I>-pSB1C3</td> |
| − | </tr> | + | </tr> |
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>oprF-3×LBT7</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td><I>oprF-3×LBT10</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>oprF-3×LBT11</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>oprF-3×LBT12</I>-pSB1C3</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| − | + | <!--*********<I>PmrC-GFP</I>-pBAD30→<I>PmrC-GFP</I>-pSB1C3**************Page21****************--> | |
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <h4><strong><I>PmrC-GFP</I>-pBAD30→<I>PmrC-GFP</I>-pSB1C3</strong> </h4> |
| − | <p><strong> | + | <p><strong>10.2</strong></p> |
| − | <p><strong> | + | <p><strong>PCR</strong></p> |
| + | <p><strong>Ziyang Xiao</strong></p> | ||
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,343: | Line 2,537: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrC-GFP</I></td> |
| − | <td> | + | <td>30 μl</td> |
| − | </tr> | + | </tr> |
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,352: | Line 2,546: | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.4</strong></p> |
<p><strong>Restriction Enzyme Digestion</strong></p> | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| − | <p><strong> | + | <p><strong>Yubei Sun</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,365: | Line 2,559: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrC-GFP</I></td> |
| − | + | <td>Pstl I and XbaI</td> | |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | </tr> | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | |||
| − | |||
</div> | </div> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.5</strong></p> |
| − | <p><strong> | + | <p><strong>DNA ligation & transformation</strong></p> |
| − | <p><strong> | + | <p><strong>Ziyang Xiao</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,402: | Line 2,584: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrC-GFP</I></td> |
| − | <td> | + | <td>pSB1C3(Pstl I and XbaI cut)</td> |
| − | <td> | + | <td>DH5a</td> |
| − | <td>LB( | + | <td>LB(chl added)</td> |
</tr> | </tr> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,421: | Line 2,595: | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.6</strong></p> |
<p><strong>Colony PCR</strong></p> | <p><strong>Colony PCR</strong></p> | ||
| − | <p><strong> | + | <p><strong>Ziyang Xiao</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,435: | Line 2,609: | ||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
| − | <td> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>2</td> |
| − | <td> | + | <td><I>PmrC-GFP</I>-pSB1C3</td> |
| − | </tr> | + | </tr> |
| − | + | <tr> | |
| + | <td>3</td> | ||
| + | <td><I>PmrC-GFP</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td><I>PmrC-GFP</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td><I>PmrC-GFP</I>-pSB1C3</td> | ||
| + | </tr> | ||
| + | |||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | |||
| − | |||
</div> | </div> | ||
| − | + | <!--****************Page 22****************************--> | |
| − | + | <div class="col-xs-12"> | |
| − | + | <h4><strong><I>PmrA-PmrB(LBT X)-PmrC-GFP</I>-pBAD30→<I>PmrA-PmrB(LBT X)-PmrC-GFP</I>-pSB1C3</strong> </h4> | |
| − | <p><strong> | + | <p><strong>10.26</strong></p> |
| − | <p><strong> | + | <p><strong>PCR</strong></p> |
| − | <p><strong> | + | <p><strong>Ziyang Xiao</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,464: | Line 2,648: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT 5)PmrC-GFP</I></td> |
| − | <td> | + | <td>30 μl</td> |
</tr> | </tr> | ||
| − | |||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT 9)-PmrC-GFP</I></td> |
| − | <td> | + | <td>30 μl</td> |
| − | </tr> | + | </tr> |
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT 11)-PmrC-GFP</I></td> |
| − | + | <td>30 μl</td> | |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | </tr> | + | |
</tbody> | </tbody> | ||
</table> | </table> | ||
| − | </div> | + | </div> |
</div> | </div> | ||
| − | |||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.4</strong></p> |
<p><strong>Restriction Enzyme Digestion</strong></p> | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| − | <p><strong> | + | <p><strong>Huiping Shi</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,524: | Line 2,678: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB（LBT 5）-PmrC-GFP</I></td> |
| − | + | <td>Pst I and XbaI</td> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
</tr> | </tr> | ||
| − | + | <tr> | |
| − | <td> | + | <td><I>PmrA-PmrB（LBT 9）-PmrC-GFP</I></td> |
| − | + | <td>Pst I and XbaI</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td><I>PmrA-PmrB（LBT 11）-PmrC-GFP</I></td> | |
| − | + | <td>Pst I and XbaI</td> | |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | </tr> | + | |
| − | + | ||
| − | <td> | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | </tr> | + | |
| − | + | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | |||
| − | |||
</div> | </div> | ||
| − | + | <div class="col-xs-12"> | |
| − | <p><strong> | + | <p><strong>10.5</strong></p> |
| − | <p><strong> | + | <p><strong>DNA ligation & transformation</strong></p> |
| − | <p><strong> | + | <p><strong>Huiping Shi</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,588: | Line 2,711: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB（LBT 5）-PmrC-GFP</I></td> |
| − | <td> | + | <td>pSB1C3(Pst I and Spel I cut)</td> |
| − | <td> | + | <td>DH5a</td> |
| − | <td>LB( | + | <td>LB(chl added)</td> |
| − | </tr> | + | </tr> |
| + | <tr> | ||
| + | <td><I>PmrA-PmrB（LBT 9）-PmrC-GFP</I></td> | ||
| + | <td>pSB1C3(Pst I and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB（LBT 11）-PmrC-GFP</I></td> | ||
| + | <td>pSB1C3(Pst I and Spel I cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,599: | Line 2,734: | ||
| − | + | ||
| − | <p><strong> | + | |
| − | <p><strong> | + | |
| − | <p><strong> | + | |
| + | <!-- yuyiyan --> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="col-xs-12"> | ||
| + | <p><strong>10.13</strong></p> | ||
| + | <p><strong>Splicing by overlap extension step 1</strong></p> | ||
| + | <p><strong>Yiyan Yu</strong></p> | ||
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | |||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| + | <th style="text-align: center;">Total</th> | ||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT5)</I></td> |
| − | <td> | + | <td>50μl</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT9)</I></td> |
| − | <td> | + | <td>50μl</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT11)</I></td> |
| − | <td> | + | <td>50μl</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrC-GFP</I></td> |
| − | + | <td>50μl</td> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | <td> | + | |
</tr> | </tr> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | <img | + | <img src="https://static.igem.org/mediawiki/2017/a/ac/2017_HUST_China_notebook_pic1.jpg" class="col-xs-6 col-xs-offset-2"> |
| + | <img src="https://static.igem.org/mediawiki/2017/4/40/2017_HUST_China_notebook_pic2.jpg" class="col-xs-6 col-xs-offset-2"> | ||
</div> | </div> | ||
| − | + | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | + | <p><strong>10.14</strong></p> | |
| − | <p><strong> | + | <p><strong>Splicing by overlap extension step 2</strong></p> |
| − | <p><strong> | + | <p><strong>Qingyang Yu</strong></p> |
| − | <p><strong> | + | |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,676: | Line 2,804: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT5)-PmrC-GFP</I></td> |
| − | <td> | + | <td>50μl</td> |
| − | </tr> | + | </tr> |
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT9)-PmrC-GFP</I></td> | ||
| + | <td>50μl</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT11)-PmrC-GFP</I></td> | ||
| + | <td>50μl</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | <img | + | <img src="https://static.igem.org/mediawiki/2017/d/db/2017_HUST_China_notebook_pic3.jpg" class="col-xs-6 col-xs-offset-2"> |
</div> | </div> | ||
| − | + | <div class="col-xs-12"> | |
| − | + | <p><strong>10.16</strong></p> | |
| − | <p><strong> | + | <p><strong>Restriction Enzyme Digestion</strong></p> |
| − | <p><strong> | + | <p><strong>Yiyan Yu</strong></p> |
| − | <p><strong> | + | |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,695: | Line 2,830: | ||
<tr> | <tr> | ||
<th style="text-align: center;">Name</th> | <th style="text-align: center;">Name</th> | ||
| − | <th style="text-align: center;"> | + | <th style="text-align: center;">Enzyme</th> |
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB（LBT 5）-PmrC-GFP</I></td> |
| − | <td> | + | <td>EcoR I & Pst I</td> |
| − | </tr> | + | </tr> |
| + | <tr> | ||
| + | <td><I>PmrA-PmrB（LBT 9）-PmrC-GFP</I></td> | ||
| + | <td>EcoR I & Pst I</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB（LBT 11）-PmrC-GFP</I></td> | ||
| + | <td>EcoR I & Pst I</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/7/73/2017_HUST_China_notebook_pic4.jpg" class="col-xs-8 col-xs-offset-2"> | ||
| + | |||
</div> | </div> | ||
| + | |||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.19</strong></p> |
<p><strong>Restriction Enzyme Digestion</strong></p> | <p><strong>Restriction Enzyme Digestion</strong></p> | ||
| − | <p><strong> | + | <p><strong>Yiyan Yu</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,722: | Line 2,868: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>psb1c3-PmrC-GFP</I></td> |
| − | + | <td>EcoR I & Pst I</td> | |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | + | ||
| − | </tr> | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2017/9/91/2017_HUST_China_notebook_pic5.jpg" class="col-xs-8 col-xs-offset-2"> | |
| − | + | ||
</div> | </div> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p><strong> | + | <p><strong>10.21</strong></p> |
| − | <p><strong> | + | <p><strong>DNA ligation & transformation</strong></p> |
| − | <p><strong> | + | <p><strong>Ziyang Xiao</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,759: | Line 2,894: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT5)-PmrC-GFP</I></td> |
| − | <td> | + | <td>pSB1C3(Pstl I and XbaI cut)</td> |
| − | <td> | + | <td>DH5a</td> |
| − | <td>LB( | + | <td>LB(chl added)</td> |
</tr> | </tr> | ||
| − | + | <tr> | |
| − | <td> | + | <td><I>PmrA-PmrB(LBT9)-PmrC-GFP</I></td> |
| − | <td> | + | <td>pSB1C3(Pstl I and XbaI cut)</td> |
| − | <td> | + | <td>DH5a</td> |
| − | <td>LB( | + | <td>LB(chl added)</td> |
| − | </tr> | + | </tr> |
| − | + | ||
| + | <tr> | ||
| + | <td><I>PmrA-PmrB(LBT11)-PmrC-GFP</I></td> | ||
| + | <td>pSB1C3(Pstl I and XbaI cut)</td> | ||
| + | <td>DH5a</td> | ||
| + | <td>LB(chl added)</td> | ||
| + | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| Line 1,777: | Line 2,918: | ||
</div> | </div> | ||
| − | + | ||
| − | <p><strong> | + | <div class="col-xs-12"> |
| + | <p><strong>10.22</strong></p> | ||
<p><strong>Colony PCR</strong></p> | <p><strong>Colony PCR</strong></p> | ||
| − | <p><strong> | + | <p><strong>Kangyuan Yu</strong></p> |
<div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | <div class="table-responsive col-xs-10 col-xs-offset-1" style="text-align: center; font-size:14px;"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
| Line 1,791: | Line 2,933: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>4</td> |
| − | <td> | + | <td><I>PmrA-PmrB(LBT5)-PmrC-GFP</I></td> |
</tr> | </tr> | ||
| + | |||
<tr> | <tr> | ||
<td>1</td> | <td>1</td> | ||
| − | <td> | + | <td><I>PmrA-PmrB(LBT9)-PmrC-GFP</I></td> |
| − | </tr> | + | </tr> |
| − | + | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td><I>PmrA-PmrB(LBT9)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td><I>PmrA-PmrB(LBT11)-PmrC-GFP</I></td> | ||
| + | </tr> | ||
| + | |||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
| − | <img | + | <img src="https://static.igem.org/mediawiki/2017/1/12/2017_HUST_China_notebook_pic6.jpg" class="col-xs-8 col-xs-offset-2" style="padding:10px;"> |
| − | + | ||
</div> | </div> | ||
| Line 1,816: | Line 2,973: | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 17:01, 1 November 2017
