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| | <p>For the expression of the Hijack, Detect and Terminate (HDT) genetic circuit it is necessary to dispose of a system to deliver effectively DNA into appropriate bacterial hosts in heterogeneous samples. The expression of synthetic circuits in bacterial hosts can be done through conventional protocols transformation. Nevertheless, a delivery system based on these protocols is not suitable on the conditions for which this HDT system has been conceived, since samples are heterogeneous and may have extremely low concentrations of target host. Also they are not suitable for its massive use in hospitals and field applications in a labour- and cost-effective manner. Therefore, we propose here a portable 3D printed device that enables the infection of the target sample with engineered synthetic bacteriophages harbouring the synthetic HDT device and allow for the delivery of DNA in heterogeneous samples with low amount of target microorganisms such as M. tuberculosis with high specificity.</p> | | <p>For the expression of the Hijack, Detect and Terminate (HDT) genetic circuit it is necessary to dispose of a system to deliver effectively DNA into appropriate bacterial hosts in heterogeneous samples. The expression of synthetic circuits in bacterial hosts can be done through conventional protocols transformation. Nevertheless, a delivery system based on these protocols is not suitable on the conditions for which this HDT system has been conceived, since samples are heterogeneous and may have extremely low concentrations of target host. Also they are not suitable for its massive use in hospitals and field applications in a labour- and cost-effective manner. Therefore, we propose here a portable 3D printed device that enables the infection of the target sample with engineered synthetic bacteriophages harbouring the synthetic HDT device and allow for the delivery of DNA in heterogeneous samples with low amount of target microorganisms such as M. tuberculosis with high specificity.</p> |
| | <object data="https://static.igem.org/mediawiki/2017/d/da/T--IISER-Pune-India--WikiIISER_Phage_1.pdf" type="application/pdf" style="width: 960px; height: 500px; margin: auto;"><embed src="https://static.igem.org/mediawiki/2017/d/da/T--IISER-Pune-India--WikiIISER_Phage_1.pdf" /></object> | | <object data="https://static.igem.org/mediawiki/2017/d/da/T--IISER-Pune-India--WikiIISER_Phage_1.pdf" type="application/pdf" style="width: 960px; height: 500px; margin: auto;"><embed src="https://static.igem.org/mediawiki/2017/d/da/T--IISER-Pune-India--WikiIISER_Phage_1.pdf" /></object> |
| | + | <center> |
| | <a href="https://2017.igem.org/File:T--IISER-Pune-India--Amplicon_LysA.png" class="button style2 big">Amplicon_LysA,LysB</a><br /> | | <a href="https://2017.igem.org/File:T--IISER-Pune-India--Amplicon_LysA.png" class="button style2 big">Amplicon_LysA,LysB</a><br /> |
| | <a href="https://2017.igem.org/File:T--IISER-Pune-India--pHAE159.png" class="button style2 big">pHAE159_Lysis cassette!</a><br /> | | <a href="https://2017.igem.org/File:T--IISER-Pune-India--pHAE159.png" class="button style2 big">pHAE159_Lysis cassette!</a><br /> |
| | <a href="https://2017.igem.org/File:PMyBADC.png" class="button style2 big">pMyBADC_Kan</a><br /> | | <a href="https://2017.igem.org/File:PMyBADC.png" class="button style2 big">pMyBADC_Kan</a><br /> |
| | <a href="https://2017.igem.org/File:TM4_from_U_pittspburgh_(featured)_Map.png" class="button style2 big">TM4_from_U_pittspburgh_(featured)_Map</a> | | <a href="https://2017.igem.org/File:TM4_from_U_pittspburgh_(featured)_Map.png" class="button style2 big">TM4_from_U_pittspburgh_(featured)_Map</a> |
| | + | </center> |
| | </article> | | </article> |
| | </div> | | </div> |
_Map.png){kind=link}