Difference between revisions of "Team:IISER-Pune-India/Attributions"

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<div class="title">Attributions</div>
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<h1>Work by the team</h1>
  
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<li class="icon fa-gratipay">The team participated in Interlab study by iGEM 2017 and submitted the data. Aarti, Avani, Zakhiya, Jyothish and Charvee participated in transformations and measurement and compilation of data using plate reader.</li>
  
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<li class="icon fa-gratipay">In growth curve experiments of DH5α and MG1655 strains of Escherichia coli, Aarti, Avani, Zakhiya, Jyothish and Charvee participated in measurement of OD at different time points.</li>
  
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<li class="icon fa-gratipay"> Aarti Kejriwal and Avani Koparkar were involved in the Hijack module and building of a modified version of the synthetic oscillator by Stricker et al. They were also in charge of organising the India Meet-up 2017 (Regional). Avani handles the twitter account of our team, and Avani and Aarti handle facebook page of our team. </li>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<li class="icon fa-gratipay">Zakhiya PC and Jyothish Sudhakaran were involved in the Detection module which included the expression of chromophores under a constitutive promoter. They also attempted to clone dual promoter of Mycobacterium tuberculosis and Escherichia coli. Zakhiya was also in charge of media and making of team video. Both of them helped in making our team logo as well.</li>
  
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<li class="icon fa-gratipay">Charvee Ravichandran was involved in Termination module which included building a quorum-sensing based technique leading to termination of growth of bacteria and/or lysis of cells.</li>
  
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<li class="icon fa-gratipay">Yash Joshi was involved in modelling of the Hijack Module based on models of synthetic oscillators given in papers by Stricker et al. (2008) and Hasty et al. (2002). He was also responsible for getting our private sponsors.</li>
  
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<li class="icon fa-gratipay">Saumil Shah was in charge of wiki design, T-shirt design, banner design, poster design and making the final presentation.</li>
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<h1>Attributions</h1>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.  
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<li class="icon fa-gratipay">In our collaboration with IISc, Bangalore iGEM team which included haemocytometry of yeast samples, Aarti, Zakhiya and Jyothish were involved in sample preparation while Jyothish and Zakhiya were involved in imaging of samples.</li>
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This is a bronze medal requirement. Please see the <a href="https://2017.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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<li class="icon fa-gratipay">Jyothish carried out most of the T7 bacteriophage experiments along with Aarti and Charvee, while the entire team helped in plaque counting.</li>
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<li class="icon fa-gratipay">Jyothish made the questionnaire for the human practice survey form.</li></ul>
  
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<h1>General support</h1>
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<ul>
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<li class="icon fa-gratipay">Prof. K. N. Ganesh, Director, IISER-Pune wholeheartedly supported the project and made sure that monetary transactions for travel, consumables and stay were smooth.</li>
  
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<li class="icon fa-gratipay">Dr. Sanjeev Galande, for talking to the students during India meet-up 2017 (Regional)</li>
  
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<li class="icon fa-gratipay">Dr. Nishad Matange, for talking to the students during India meet-up 2017 (Regional)</li>
  
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<li class="icon fa-gratipay">Dr. Sandeep Krishna, NCBS for talking to the students during India meet-up 2017 (Regional)</li>
  
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<li class="icon fa-gratipay">Robert Ramirez Garcia, for talking to the students during India meet-up 2017 (Regional)</li>
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<h5> Why is this page needed? </h5>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<li class="icon fa-gratipay">Dr. Neelesh Dahanukar</li>
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<li class="icon fa-gratipay">UG lab TAs: Poornima Raveendran, Prabha Desai and Manjiri Kekatpure</li>
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<li class="icon fa-gratipay">Vivek Kanadi, Pradeep sir, Science media center, IISER-Pune</li>
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<li class="icon fa-gratipay">IISER-Pune housekeeping staff</li>
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<li class="icon fa-gratipay">Siddharth Adithyan: BS-MS student for designing the logo</li>
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<li class="icon fa-gratipay">Himanshu Lagachu: BS-MS student for making the team video</li>
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<li class="icon fa-gratipay">Kaustubh Amritkar and team : BS-MS student for arranging Skype session during India Meet-up 2017 (Regional)</li></ul>
  
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<h1>Project support and advice</h1>
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<h5> What should this page have?</h5>
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<ul>
 
<ul>
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<li>General Support</li>
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<li class="icon fa-gratipay">Dr. Chaitanya Athale: PI, advised us throughout the project. He oversaw and helped in plate reader protocols, PCR protocols, cloning and provided useful insights in modelling of the oscillator.</li>  
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<li>Project support and advice</li>
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<li class="icon fa-gratipay">Kunalika Jain: PhD student of Dr Chaitanya Athale who advised us in various experiments and helped us in techniques like gel electrophoresis and plate reader</li>
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<li>Fundraising help and advice</li>
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<li class="icon fa-gratipay">Yash Jawale: Former member of 2015 iGEM IISER-Pune team who advised us throughput out the project, helped us in experiments like haemocytometry, and also assisted in microscopy, plate reader, gel electrophoresis. He helped in debugging the program simulating the model of the oscillator. He also participated in organising the iGEM India meet-up 2017 (Regional)</li>
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<li>Lab support</li>
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<li class="icon fa-gratipay">Snehal Kadam: Former member of 2015 iGEM IISER-Pune team who helped us in troubleshooting lab protocols. She also provided insight into promoting our project via facebook and twitter accounts.</li>
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<li>Difficult technique support</li>
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<li class="icon fa-gratipay">Robert Ramirez Garcia: Former member of the iGEM 2016 Évry team, in France, who taught us experiments in phage kinetics and host dynamics and also advised us on our project. He visited our institute and helped us perform all the T7 Bacteriophage experiments for a week. </li>
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<li>Project advisor support</li>
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<li class="icon fa-gratipay">iGEM 2015 team of IISER Pune for conceptualizing and providing basis for our project.</li>
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<li>Wiki support</li>
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<li class="icon fa-gratipay">Saptarshi SohamMohonta, first year, BS-MS student for making the 3D model of our prototype (digitized)</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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<h1>Fundraising help and advice</h1>
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<h5>Inspiration</h5>
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<p>Take a look at what other teams have done:</p>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li class="icon fa-gratipay">Our institute, IISER-Pune sponsored the team registration, consumables as well as travel and registration of one team member.</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li class="icon fa-gratipay">Jayant Joshi helped us in contacting several potential private sponsors.</li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
  
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<h1>Project advisor support</h1>
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<ul><li class="icon fa-gratipay">Dr. Vineeta Bal, IISER-Pune: Secondary PI, who helped us fine-tune our project with her expertise in TB diagnostics. She also supervised the organization of India Meet-up 2017 (Regional)</li>
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<div class="clear"></div>
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<li class="icon fa-gratipay">Dr. Nishad Matange, IISER-Pune</li>
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<li class="icon fa-gratipay">Dr. Amrita Hazra, IISER-Pune</li>
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Presentation coaching
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<li class="icon fa-gratipay">Dr. Vineeta Bal</li>
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<li class="icon fa-gratipay">Dr. Chaitanya Athale</li>
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<li class="icon fa-gratipay">Kunalika Jain, Athale lab</li>
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<li class="icon fa-gratipay">Yash Jawale, BS-MS student, Athale lab</li>
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<li class="icon fa-gratipay">Neha Ketan, Athale lab</li>
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<li class="icon fa-gratipay">Snehal Kadam, BS-MS student, Athale lab</li>
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<li class="icon fa-gratipay">Tanmaya Sethi, BS-MS student, Athale lab</li>
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</ul>
  
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<h5>Team training and Project start</h5>
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<h1>Difficult experiment support</h1>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li class="icon fa-gratipay">Robert Ramirez Garcia: Phage engineer and former member of the iGEM 2016 Évry team. He taught and assisted us in experiments in understanding the kinetics of infection with the T7 bacteriophage and its host dynamics. He also designed genome-minimised conditionally replicating phages for the amplification, delivery and expression of our synthetic construct in Escherichia coli (through a λ-like enterobacteria phage) and Mycobacterium spp (through a TM4-like mycobacteriophage).</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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<h1>Human Practices support</h1>
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<ul><li class="icon fa-gratipay">Lupin ltd for scheduling a project presentation and providing insights into our project</li>
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<li class="icon fa-gratipay">Anushree Chaphalkar, for arranging our first Human Practices talk with school students from Kolhapur</li>
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<li class="icon fa-gratipay">Karavaan, IISER-Pune for arranging student talks related to synthetic biology and our iGEM project.</li>
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<li class="icon fa-gratipay">We thank all iGEM 2017 teams from India as well as iGEM 2017 team from Peshawar, Pakistan for attending the India meet-up 2017 (Regional) and actively participating in discussions. We would also like to thank them for their constant support on various social media platforms and the invaluable experimental advice they provided.</li>
 
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{{IISER-Pune-India/footer}}

Latest revision as of 03:14, 2 November 2017

Attributions

Work by the team

  • The team participated in Interlab study by iGEM 2017 and submitted the data. Aarti, Avani, Zakhiya, Jyothish and Charvee participated in transformations and measurement and compilation of data using plate reader.
  • In growth curve experiments of DH5α and MG1655 strains of Escherichia coli, Aarti, Avani, Zakhiya, Jyothish and Charvee participated in measurement of OD at different time points.
  • Aarti Kejriwal and Avani Koparkar were involved in the Hijack module and building of a modified version of the synthetic oscillator by Stricker et al. They were also in charge of organising the India Meet-up 2017 (Regional). Avani handles the twitter account of our team, and Avani and Aarti handle facebook page of our team.
  • Zakhiya PC and Jyothish Sudhakaran were involved in the Detection module which included the expression of chromophores under a constitutive promoter. They also attempted to clone dual promoter of Mycobacterium tuberculosis and Escherichia coli. Zakhiya was also in charge of media and making of team video. Both of them helped in making our team logo as well.
  • Charvee Ravichandran was involved in Termination module which included building a quorum-sensing based technique leading to termination of growth of bacteria and/or lysis of cells.
  • Yash Joshi was involved in modelling of the Hijack Module based on models of synthetic oscillators given in papers by Stricker et al. (2008) and Hasty et al. (2002). He was also responsible for getting our private sponsors.
  • Saumil Shah was in charge of wiki design, T-shirt design, banner design, poster design and making the final presentation.
  • In our collaboration with IISc, Bangalore iGEM team which included haemocytometry of yeast samples, Aarti, Zakhiya and Jyothish were involved in sample preparation while Jyothish and Zakhiya were involved in imaging of samples.
  • Jyothish carried out most of the T7 bacteriophage experiments along with Aarti and Charvee, while the entire team helped in plaque counting.
  • Jyothish made the questionnaire for the human practice survey form.

General support

  • Prof. K. N. Ganesh, Director, IISER-Pune wholeheartedly supported the project and made sure that monetary transactions for travel, consumables and stay were smooth.
  • Dr. Sanjeev Galande, for talking to the students during India meet-up 2017 (Regional)
  • Dr. Nishad Matange, for talking to the students during India meet-up 2017 (Regional)
  • Dr. Sandeep Krishna, NCBS for talking to the students during India meet-up 2017 (Regional)
  • Robert Ramirez Garcia, for talking to the students during India meet-up 2017 (Regional)
  • Dr. Neelesh Dahanukar
  • UG lab TAs: Poornima Raveendran, Prabha Desai and Manjiri Kekatpure
  • Vivek Kanadi, Pradeep sir, Science media center, IISER-Pune
  • IISER-Pune housekeeping staff
  • Siddharth Adithyan: BS-MS student for designing the logo
  • Himanshu Lagachu: BS-MS student for making the team video
  • Kaustubh Amritkar and team : BS-MS student for arranging Skype session during India Meet-up 2017 (Regional)

Project support and advice

  • Dr. Chaitanya Athale: PI, advised us throughout the project. He oversaw and helped in plate reader protocols, PCR protocols, cloning and provided useful insights in modelling of the oscillator.
  • Kunalika Jain: PhD student of Dr Chaitanya Athale who advised us in various experiments and helped us in techniques like gel electrophoresis and plate reader
  • Yash Jawale: Former member of 2015 iGEM IISER-Pune team who advised us throughput out the project, helped us in experiments like haemocytometry, and also assisted in microscopy, plate reader, gel electrophoresis. He helped in debugging the program simulating the model of the oscillator. He also participated in organising the iGEM India meet-up 2017 (Regional)
  • Snehal Kadam: Former member of 2015 iGEM IISER-Pune team who helped us in troubleshooting lab protocols. She also provided insight into promoting our project via facebook and twitter accounts.
  • Robert Ramirez Garcia: Former member of the iGEM 2016 Évry team, in France, who taught us experiments in phage kinetics and host dynamics and also advised us on our project. He visited our institute and helped us perform all the T7 Bacteriophage experiments for a week.
  • iGEM 2015 team of IISER Pune for conceptualizing and providing basis for our project.
  • Saptarshi SohamMohonta, first year, BS-MS student for making the 3D model of our prototype (digitized)

Fundraising help and advice

  • Our institute, IISER-Pune sponsored the team registration, consumables as well as travel and registration of one team member.
  • Jayant Joshi helped us in contacting several potential private sponsors.

Project advisor support

  • Dr. Vineeta Bal, IISER-Pune: Secondary PI, who helped us fine-tune our project with her expertise in TB diagnostics. She also supervised the organization of India Meet-up 2017 (Regional)
  • Dr. Nishad Matange, IISER-Pune
  • Dr. Amrita Hazra, IISER-Pune
    • Presentation coaching
  • Dr. Vineeta Bal
  • Dr. Chaitanya Athale
  • Kunalika Jain, Athale lab
  • Yash Jawale, BS-MS student, Athale lab
  • Neha Ketan, Athale lab
  • Snehal Kadam, BS-MS student, Athale lab
  • Tanmaya Sethi, BS-MS student, Athale lab

Difficult experiment support

  • Robert Ramirez Garcia: Phage engineer and former member of the iGEM 2016 Évry team. He taught and assisted us in experiments in understanding the kinetics of infection with the T7 bacteriophage and its host dynamics. He also designed genome-minimised conditionally replicating phages for the amplification, delivery and expression of our synthetic construct in Escherichia coli (through a λ-like enterobacteria phage) and Mycobacterium spp (through a TM4-like mycobacteriophage).

Human Practices support

  • Lupin ltd for scheduling a project presentation and providing insights into our project
  • Anushree Chaphalkar, for arranging our first Human Practices talk with school students from Kolhapur
  • Karavaan, IISER-Pune for arranging student talks related to synthetic biology and our iGEM project.
  • We thank all iGEM 2017 teams from India as well as iGEM 2017 team from Peshawar, Pakistan for attending the India meet-up 2017 (Regional) and actively participating in discussions. We would also like to thank them for their constant support on various social media platforms and the invaluable experimental advice they provided.