Difference between revisions of "Team:IISER-Pune-India/HP/Gold Integrated"

 
(12 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{IISER-Pune-India}}
 
{{IISER-Pune-India}}
−
{{IISER-Pune-India/navbar|title=Cherries?|tl1=Taste the icing on the cake!|tl2=IISER Pune India iGEM 2017 Wiki.}}
+
{{IISER-Pune-India/navbar|title=Integrated Human Practices|tl1=The more you share the knowledge|tl2=the more you learn}}
 
<html>
 
<html>
 
<body class="no-sidebar">
 
<body class="no-sidebar">
 
<!-- Main -->
 
<!-- Main -->
 
<div class="wrapper style2">
 
<div class="wrapper style2">
−
<div class="title">Medal Criteria</div>
+
<div class="title">Integrated Human Practices(Gold)</div>
 
<div id="main" class="container">
 
<div id="main" class="container">
 
<!-- Content -->
 
<!-- Content -->
Line 17: Line 17:
 
<div class="column full_size">
 
<div class="column full_size">
  
−
<h1>Gold Medal and Integrated Human Practices</h1>
+
<h1>Integrated Human Practices</h1>
  
−
<p>This page will contain information for your Gold medal Human Practices work, which you can also use to nominate your team for the Best Integrated Human Practices page. To make things easier, we have combined the Gold medal page with the Best Integrated Human Practices page since we expect the work to overlap considerably. </p>
+
<h3>Prototype</h3>
−
<p>iGEM teams are unique and leading the field because they "go beyond the lab" to imagine their projects in a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
+
<p>The device prototype is designed in such a way that it ensures the physical containment of the construct. It is similar to a 50mL falcon tube with a one way lock which ensures that the device cannot be opened once it is capped tightly. This device will contain the phasmids that can infect Mycobacterium and dispense the gene circuits into them along with middle brook media containing IPTG and Arabinose, all of which is separated from the exterior by an insoluble membrane. After adding the pathological sample into the device, a small tube will be added to the device to pierce the membrane on closing the cap. This ensures the mixing of the media in the device with the pathological sample.</p>
−
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
+
<div class="row 150%">
−
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
+
<div class="2u">
−
</div>
+
<section class="box"><img src="https://static.igem.org/mediawiki/2017/9/9a/T--IISER-Pune-India--Prototype.png" width="200px"/></section></div></div><br />
−
<div class="clear"></div>
+
  
 +
<h3>Biosafety Module</h3>
 +
<p>One of the major concerns raised by many of our audience was the accidental chances of the device getting released into the wild. In-order to ensure the biosafety of the module in cases of such accidents,  we are planning to incorporate a biosafety module in our device. The biosafety module involves a Nuclease, which chews up the DNA and RNA of the host when activated. This nuclease will be under the control of a highly repressed promoter, repressed by a protein R. This protein R will be under the control of an inducible promoter. Our device will contain the inducer for this inducible promoter which will cause the transcription of gene R and in-turn will inhibit the production of nucleases. So our constructs will be stable in our device. In case of plasmid getting lost into the environment, the gene R won’t be induced and thus will cause the expression of nucleases which will digest the DNA of the host organism including the detection device, thereby ensuring the biosafety of the device. As of now a possible candidate for R gene is Monococcal nuclease. It will be under the control of p(TetR) promoter. TetR coding sequence will be expressed under the control of pLac promoter which is induced by IPTG.
 +
</p>
 +
<div class="row 150%">
 +
<div class="2u">
 +
<section class="box"><img src="https://static.igem.org/mediawiki/2017/9/95/T--IISER-Pune-India--BiosafetyMap.png" width="200px"/></section></div></div><br />
 +
<h3>Expert Advice</h3>
 +
<p>One of our private sponsors is Lupin Limited which is a leading pharmaceutical company. We visited Lupin Research Park in Pune in order to collect expert advice in improving the safety of our module. We gave a presentation describing our project,  to some of the industry experts, researchers, doctors working in the field of TB research. They gave us valuable feedback on the feasibility and relevance of our project. We got positive criticism from them which helped us in looking at our project with a new perspective. They suggested that we should consider attenuating bacteria before making their replication faster as an added biosafety measure. They also talked about extrapulmonary tuberculosis.
 +
We took a feedback after the presentation which included the following questions and the response is represented in the form graphs. </p>
 +
<p> From L-R each row, the questions corresponding to the graphs below are:</p>
 +
<ul>
 +
<li>Q.1 How relevant do you think our project is in the field of TB?</li>
 +
<li>Q.2 What do you think about the feasibility of our project?</li>
 +
<li>Q.3 How safe do you think our project will be inthe real world?</li>
 +
<li>Q.4 How much would you rely on such a diagnostic tool?</li>
 +
<li>Q.5 What is the potential for commercialization of our diagnostic tool?</li>
 +
<li>Q.6 Any other comments and suggestions regarding safety, etc?</li>
 +
</ul> 
  
−
<div class="column half_size">
+
<div class="row 150%">
−
<h3>Gold Medal Criterion #1</h3>
+
                    <div class="6u 6u(mobile)">
−
<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.</p>
+
                        <section class="box">
 +
                          <a href="#" class="image featured">
 +
                              <img src="https://static.igem.org/mediawiki/2017/f/f1/T--IISER-Pune-India--q1.png">
 +
                          </a><p><i>Question 1</i></p>
 +
</section></div>
 +
<div class="6u 6u(mobile)">
 +
                        <section class="box">
 +
<a href="#" class="image featured">
 +
                              <img src="https://static.igem.org/mediawiki/2017/e/e7/T--IISER-Pune-India--q2.png">
 +
                          </a><p><i>Question 2</i></p></section></div>
 +
<div class="6u 6u(mobile)">
 +
                        <section class="box">
 +
<a href="#" class="image featured">
 +
                              <img src="https://static.igem.org/mediawiki/2017/c/c5/T--IISER-Pune-India--q3.png">
 +
                          </a><p><i>Question 3</i></p></section></div>
 +
<div class="6u 6u(mobile)">
 +
                        <section class="box">
 +
<a href="#" class="image featured">
 +
                              <img src="https://static.igem.org/mediawiki/2017/0/0d/T--IISER-Pune-India--q4.png">
 +
                          </a><p><i>Question 4</i></p></section></div>
 +
<div class="6u 6u(mobile)">
 +
                        <section class="box">
 +
<a href="#" class="image featured">
 +
                              <img src="https://static.igem.org/mediawiki/2017/b/bd/T--IISER-Pune-India--q5.png">
 +
                          </a> <p><i>Question 5</i></p></section>
 +
                    </div>
 +
                  </div>
  
−
</div>
 
  
−
<div class="column half_size">
 
−
<h3>Best Integrated Human Practices Special Prize</h3>
 
  
−
<p>
+
</article>
−
To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Integrated Human Practices prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
+
−
<br><br>
+
−
You must also delete the message box on the top of this page to be eligible for this prize.
+
−
</p>
+
−
</div>
+
−
<div class="clear"></div>
+
−
<div class="column full_size">
+
−
<h5>Inspiration</h5>
+
−
<p>Here are a few examples of excellent Integrated Human Practices work:</p>
+
−
<ul>
+
−
<li><a href="https://2016.igem.org/Team:INSA-Lyon/Integrated_Practices">2016 INSA Lyon</a></li>
+
−
<li><a href="https://2016.igem.org/Team:UofC_Calgary/Integrated_Practices">2016 UofC Calgary</a></li>
+
−
<li><a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Practices">2015 Bielefeld</a></li>
+
−
<li><a href="https://2015.igem.org/Team:Edinburgh/Practices">2015 Edinburgh</a></li>
+
−
</ul></article>
+
 
</div>
 
</div>
 
</div>
 
</div>

Latest revision as of 03:12, 2 November 2017

Integrated Human Practices(Gold)

Integrated Human Practices

Prototype

The device prototype is designed in such a way that it ensures the physical containment of the construct. It is similar to a 50mL falcon tube with a one way lock which ensures that the device cannot be opened once it is capped tightly. This device will contain the phasmids that can infect Mycobacterium and dispense the gene circuits into them along with middle brook media containing IPTG and Arabinose, all of which is separated from the exterior by an insoluble membrane. After adding the pathological sample into the device, a small tube will be added to the device to pierce the membrane on closing the cap. This ensures the mixing of the media in the device with the pathological sample.


Biosafety Module

One of the major concerns raised by many of our audience was the accidental chances of the device getting released into the wild. In-order to ensure the biosafety of the module in cases of such accidents, we are planning to incorporate a biosafety module in our device. The biosafety module involves a Nuclease, which chews up the DNA and RNA of the host when activated. This nuclease will be under the control of a highly repressed promoter, repressed by a protein R. This protein R will be under the control of an inducible promoter. Our device will contain the inducer for this inducible promoter which will cause the transcription of gene R and in-turn will inhibit the production of nucleases. So our constructs will be stable in our device. In case of plasmid getting lost into the environment, the gene R won’t be induced and thus will cause the expression of nucleases which will digest the DNA of the host organism including the detection device, thereby ensuring the biosafety of the device. As of now a possible candidate for R gene is Monococcal nuclease. It will be under the control of p(TetR) promoter. TetR coding sequence will be expressed under the control of pLac promoter which is induced by IPTG.


Expert Advice

One of our private sponsors is Lupin Limited which is a leading pharmaceutical company. We visited Lupin Research Park in Pune in order to collect expert advice in improving the safety of our module. We gave a presentation describing our project, to some of the industry experts, researchers, doctors working in the field of TB research. They gave us valuable feedback on the feasibility and relevance of our project. We got positive criticism from them which helped us in looking at our project with a new perspective. They suggested that we should consider attenuating bacteria before making their replication faster as an added biosafety measure. They also talked about extrapulmonary tuberculosis. We took a feedback after the presentation which included the following questions and the response is represented in the form graphs.

From L-R each row, the questions corresponding to the graphs below are:

  • Q.1 How relevant do you think our project is in the field of TB?
  • Q.2 What do you think about the feasibility of our project?
  • Q.3 How safe do you think our project will be inthe real world?
  • Q.4 How much would you rely on such a diagnostic tool?
  • Q.5 What is the potential for commercialization of our diagnostic tool?
  • Q.6 Any other comments and suggestions regarding safety, etc?

Question 1

Question 2

Question 3

Question 4

Question 5