Difference between revisions of "Team:LUBBOCK TTU"

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<font face="Arial" size="6"><center>Project Aims</center></font><hr>
 
<font face="Arial" size="6"><center>Project Aims</center></font><hr>
</br><font face="Arial" size="4">- For aim 1, we will use eGFP tagged TRPV1 to confirm expression and localization.
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</br><font face="Arial" size="4">For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for In-Silico control in future work.
</br>- For aim 2, we will use calcium imaging to confirm functionality of TRPV1.
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</br>- For aim 3, we will determine conditions of reporter expression with and without TRPV1 expression.
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</br></br>We also further developed a deterministic model for the calcium pathway in yeast the stochastic nature of ion channel gating. These models will help to guide future work with TRPV1 as a thermal actuator.
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</br></br><a href="">To see our results, click here.</a>
 
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Revision as of 03:35, 19 October 2017











Characterization of a Thermal

Actuator and Calcium-Sensitive Reporter in

Saccharomyces cerevisiae
















Project Description


Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of In-Silico control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels in a range of temperatures.

To learn more about our project, click here.





Project Aims


For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for In-Silico control in future work.

We also further developed a deterministic model for the calcium pathway in yeast the stochastic nature of ion channel gating. These models will help to guide future work with TRPV1 as a thermal actuator.

To see our results, click here.



Human Practices


Description 2





Collaborations


Description 3





InterLab


Description 4





About Us


The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students.

To learn more about our team, click here.






Our Sponsors