Difference between revisions of "Team:LUBBOCK TTU"

 
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     <a href="https://2017.igem.org/Team:LUBBOCK_TTU" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HOME</font></a>
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    <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">TEAM</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">COLLABORATIONS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESCRIPTION</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESIGN</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Experiments" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">EXPERIMENTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/InterLab" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTERLAB</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Contribution" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">CONTRIBUTION</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Results" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">RESULTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Demonstrate" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DEMONSTRATE</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Attributions" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ATTRIBUTIONS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Parts" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PARTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Basic_Part" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">BASIC PARTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Silver" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SILVER HP</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Gold_Integrated" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTEGRATED AND GOLD</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Engagement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PUBLIC ENGAGEMENT</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Applied_Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">APPLIED DESIGN</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Entrepreneurship" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ENTREPRENEURSHIP</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Hardware" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HARDWARE</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Measurement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MEASUREMENT</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Plant" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PLANT</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Software" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SOFTWARE</font></a>
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<i class="fa fa-flask" style="font-size:px;color:white;"></i></br>Characterization of a Calcium-Sensitive Reporter in</br><i>Saccharomyces cerevisiae</i></br></br></center></font></p>
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</br></br></br></br></br></br></br></br</br></br></br>
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<center>Characterization of a Thermal</br></br>Actuator and Calcium-Sensitive Reporter in</br></br>Saccharomyces cerevisiae</center></font></p>
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<font face="Arial" size="6"><center>Project Description</center></font><hr>
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     <div class="col-sm-6"><font face="Arial" size="4">Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of <i>In-Silico</i> control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in <i>Saccharomyces cerevisiae</i> to monitor gene expression levels within a range of temperatures.
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    <div class="col-sm-4"><font face="Arial" size="5">Project Description</font></br></br><font face="Arial" size="2">The most commonly used input signals in iGEM generally include chemicals and light. Temperature-based input signals are not used as frequently and remain a reservoir of potential for future synthetic biology tools. Our project explores the characterization and standardization of thermosensitive ion channels, known as TRPV1, to function as a thermal actuator and regulate calcium-sensitive gene expression in Saccharomyces cerevisiae. At the activation</div>
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</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">Learn more &#8594;</a></center></font></div>
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<video width="450" height="300" controls><source src="https://static.igem.org/mediawiki/2017/1/10/Lubbock_TTU_animation.mp4" type="video/mp4"></video>  
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temperature, gating of the thermal actuator allows for an influx of extracellular calcium ions, which initiate the calmodulin-calcineurin signaling pathway, and promote the expression of genes regulated by Crz1p-sensitive promoters. The characterization of the thermal actuator was conducted in S. cerevisiae because yeast offers an accessible eukaryotic chassis for other iGEM teams to work with. By varying the temperature, the thermal actuator can be used to control genetic circuits that function as temperature-sensitive kill switches, drug delivery devices, or biosensors. <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">Learn more.</a></font></div>
 
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<font face="Arial" size="5"><center>Project Aims</center></font>
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<font face="Arial" size="6"><center>Human Practices</center></font><hr>
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</br></br>Description
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</br><font face="Arial" size="4">Human Practices is an integral part of creating impactful relationships with our community, and conducting research that is embraced by a generalized audience. The iGEM competition is centered around synthetic biology, of which includes genetic engineering, which traditionally has been a controversial area of research. Careful consideration of Human Practices ensures that our research is ethical, responsible, safe, secure, and sustainable.
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</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Silver">Learn more &#8594;</a>
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<font face="Arial" size="5">Learn More</font>
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<font face="Arial" size="6"><center>InterLab Study</center></font><hr>
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</br><font face="Arial" size="4">The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. The objective of the 2017 InterLab Study is to determine absolute units for measuring green fluorescent protein (GFP) that are easily comparable regardless of the type of plate reader used to make the measurement. We used a standardized protocol and analysis method for measuring GFP with a BioTek Synergy 4 plate reader.
  
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</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/InterLab">View 2017 InterLab Study results &#8594;</a></center></font></br></br>
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<div class="col-sm-5"><font face="Arial" size="6">About Us</font><hr></br><font face="Arial" size="4">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the Lubbock_TTU team is composed of 10 undergraduate and graduate students.</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">Meet the Team &#8594;</a></center></font></div>
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<div class="col-sm-12"><font face="Arial" size="6"><center>Collaborations</center></font><hr></div>
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</br><p>Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.</p>
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<p>This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Applied_Design">Applied Design</a> page for more information on how to compete for that award.</p>
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://static.igem.org/mediawiki/2017/9/90/Lubbock_TTU_collaborations.jpg" width="230" height="230"></figure></a>
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<h5 align="left">What should this page contain?</h5>
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<p>- Explanation of the engineering principles your team used in your design
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</br>- Discussion of the design iterations your team went through
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://www.tacc.utexas.edu/documents/1084364/1085221/utaustin-seal.png/2254124a-1d13-44cc-8e3f-6d925af2391d?t=1412720755000" width="230" height="230"></figure></a>
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</br>- Experimental plan to test your designs</p>
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<h5 align="left">Inspiration</h5>
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<p>- <a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a>
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</br>- <a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a>
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://static.igem.org/mediawiki/2017/thumb/1/17/T--Erlangen_Nuremberg--Logo.png/240px-T--Erlangen_Nuremberg--Logo.png" width="230" height="230"></figure></a>
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</br>- <a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a>
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</br><p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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<p>Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.</p>
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<p>- <a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia</a>
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</br>- <a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial</a>
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</br>- <a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech</a></p>
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</br><p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
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<p><b>Gold Medal Criterion #3</b></br>
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<p>Please see the <a href="https://2017.igem.org/Judging/Medals"> 2017 Medals Page</a> for more information. </p>
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Model prize</a>, please describe your work on this page  and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. Please note you can compete for both the gold medal criterion #3 and the best model prize with this page.
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You must also delete the message box on the top of this page to be eligible for the Best Model Prize.
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<p><b> Inspiration</b></br>
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- <a href="https://2016.igem.org/Team:Manchester/Model">Manchester 2016</a>
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</br>- <a href="https://2016.igem.org/Team:TU_Delft/Model">TU Delft 2016
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</br>- <a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a>
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- Clearly and objectively describe the results of your work.
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- Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
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</br>- Show data, but remember all measurement and characterization data must be on part pages in the Registry.
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</br>- Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.</p>
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- You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</br>
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<br>- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</p>
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<p><b>Inspiration</b></br>
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See how other teams presented their results.
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</br>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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</br>- <a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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    <div class="col-sm-4"><font face="Arial" size="5"></br></br>About Us</font></br></br><font face="Arial" size="3">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. </br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">To learn more about our team, click here.</a></font></div>
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Latest revision as of 17:38, 12 December 2017



Characterization of a Calcium-Sensitive Reporter in
Saccharomyces cerevisiae



Project Description

Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of In-Silico control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures.

Learn more →




Human Practices

Human Practices is an integral part of creating impactful relationships with our community, and conducting research that is embraced by a generalized audience. The iGEM competition is centered around synthetic biology, of which includes genetic engineering, which traditionally has been a controversial area of research. Careful consideration of Human Practices ensures that our research is ethical, responsible, safe, secure, and sustainable.

Learn more →


InterLab Study

The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. The objective of the 2017 InterLab Study is to determine absolute units for measuring green fluorescent protein (GFP) that are easily comparable regardless of the type of plate reader used to make the measurement. We used a standardized protocol and analysis method for measuring GFP with a BioTek Synergy 4 plate reader.

View 2017 InterLab Study results →



About Us

The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the Lubbock_TTU team is composed of 10 undergraduate and graduate students.

Meet the Team →



Collaborations
© 2017 Lubbock_TTU