Difference between revisions of "Team:LUBBOCK TTU"

 
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{{LUBBOCK_TTU/fontawesome/css/font-awesome.css}}
 
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
 
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#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
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<body>
 
<body>
  
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<div style="background-image: url(https://static.igem.org/mediawiki/2017/9/9e/Lubbock_TTU_header.png);>
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<p align="center><font face="Arial" size="6" color="white">
 
<center>
 
<center>
<div style="width:1110px;" style="text-align: center;>
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<div style="line-height:200%;">
  <div class="w3-bar w3-light-grey">
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     <br>
     <a href="https://2017.igem.org/Team:LUBBOCK_TTU" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HOME</font></a>
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    <div class="w3-dropdown-hover">
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      <button class="w3-button"><font face="Arial" size="2" color="black">TEAM</font></button>
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      <div class="w3-dropdown-content w3-bar-block w3-card-4">
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    <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">TEAM</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">COLLABORATIONS</font></a>
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      </div>
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    </div>       
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    <div class="w3-dropdown-hover">
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      <button class="w3-button"><font face="Arial" size="2" color="black">PROJECT</font></button>
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      <div class="w3-dropdown-content w3-bar-block w3-card-4">
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESCRIPTION</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESIGN</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Experiments" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">EXPERIMENTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Notebook" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">NOTEBOOK</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/InterLab" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTERLAB</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Contribution" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">CONTRIBUTION</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Results" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">RESULTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Demonstrate" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DEMONSTRATE</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Improve" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">IMPROVE</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Attributions" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ATTRIBUTIONS</font></a>
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      <button class="w3-button"><font face="Arial" size="2" color="black">PARTS</font></button>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Parts" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PARTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Basic_Part" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">BASIC PARTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Composite_Part" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">COMPOSITE PARTS</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Part_Collection" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PART COLLECTION</font></a>
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  </div>
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    <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Safety" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SAFETY</font></a>
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    <div class="w3-dropdown-hover">
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      <button class="w3-button"><font face="Arial" size="2" color="black">HUMAN PRACTICES</font></button>
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      <div class="w3-dropdown-content w3-bar-block w3-card-4">
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Silver" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SILVER HP</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Gold_Integrated" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTEGRATED AND GOLD</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Engagement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PUBLIC ENGAGEMENT</font></a>
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      </div>
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  </div>
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    <div class="w3-dropdown-hover">
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      <button class="w3-button"><font face="Arial" size="2" color="black">AWARDS</font></button>
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      <div class="w3-dropdown-content w3-bar-block w3-card-4">
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Applied_Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">APPLIED DESIGN</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Entrepreneurship" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ENTREPRENEURSHIP</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Hardware" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HARDWARE</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Measurement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MEASUREMENT</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Plant" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PLANT</font></a>
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        <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Software" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SOFTWARE</font></a>
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      </div>
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  </div>
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</div>
 
</div>
</center>
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<i class="fa fa-flask" style="font-size:px;color:white;"></i></br>Characterization of a Calcium-Sensitive Reporter in</br><i>Saccharomyces cerevisiae</i></br></br></center></font></p>
 
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<!-- HEADER -->
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<!--<img src="https://placehold.it/1120x570">-->
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</br><div style="background-image: url(http://www.powerpointhintergrund.com/uploads/chemistry-background-ppt-0.jpg);>
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<p align="center><font face="Arial" size="10" color="white">
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</br></br></br></br></br></br></br></br</br></br></br>
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<center>Characterization of a Thermal</br></br>Actuator and Calcium-Sensitive Reporter in</br></br>Saccharomyces cerevisiae</center></font></p>
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</br></br></br></br></br></br></br></br></br></br></br></br>
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</div>
 
</div>
 
 
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<div class="container"><!-- For NAVI -->
  
 
<!-- PROJECT DESCRIPTION -->
 
<!-- PROJECT DESCRIPTION -->
<div class="container-fluid">
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<div style="background-color:#fff; overflow: hidden;">
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<div class="col-sm-12">
 
</br></br>
 
</br></br>
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<font face="Arial" size="6"><center>Project Description</center></font><hr>
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</br><font face="Arial" size="4">
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   <div class="row">
 
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     <div class="col-sm-6"><font face="Arial" size="4">Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of <i>In-Silico</i> control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in <i>Saccharomyces cerevisiae</i> to monitor gene expression levels within a range of temperatures.  
    <div class="col-sm-4"><font face="Arial" size="6">Project Description</font></br></br><font face="Arial" size="4">Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of <i>In-Silico</i> control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels in a range of temperatures.  
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</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">Learn more &#8594;</a></center></font></div>
</br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">To learn more about our project, click here.</a></font></div>
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     <div class="col-sm-6">  
     <div class="col-sm-1"></div>
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<video width="450" height="300" controls><source src="https://static.igem.org/mediawiki/2017/1/10/Lubbock_TTU_animation.mp4" type="video/mp4"></video>  
    <div class="col-sm-4"></br></br><img src="http://via.placeholder.com/450x300">
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</br></br>
 
</br></br>
<font face="Arial" size="6"><center>Project Aims</center></font><hr>
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<font face="Arial" size="6"><center>Human Practices</center></font><hr>
</br><font face="Arial" size="4">- For aim 1, we will use eGFP tagged TRPV1 to confirm expression and localization.
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</br><font face="Arial" size="4">Human Practices is an integral part of creating impactful relationships with our community, and conducting research that is embraced by a generalized audience. The iGEM competition is centered around synthetic biology, of which includes genetic engineering, which traditionally has been a controversial area of research. Careful consideration of Human Practices ensures that our research is ethical, responsible, safe, secure, and sustainable.  
</br>- For aim 2, we will use calcium imaging to confirm functionality of TRPV1.
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</br>- For aim 3, we will determine conditions of reporter expression with and without TRPV1 expression.
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</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Silver">Learn more &#8594;</a>
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</font></br></br></br>
 
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</div>
 
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<font face="Arial" size="6"><center>InterLab Study</center></font><hr>
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</br><font face="Arial" size="4">The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. The objective of the 2017 InterLab Study is to determine absolute units for measuring green fluorescent protein (GFP) that are easily comparable regardless of the type of plate reader used to make the measurement. We used a standardized protocol and analysis method for measuring GFP with a BioTek Synergy 4 plate reader.
 +
 
 +
</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/InterLab">View 2017 InterLab Study results &#8594;</a></center></font></br></br>
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</div>
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</div>
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<!-- ABOUT US -->
 
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</br>
 
</br>
 
   <div class="row">
 
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    <div class="col-sm-1"></div>
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<div class="col-sm-5"><font face="Arial" size="6">About Us</font><hr></br><font face="Arial" size="4">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the Lubbock_TTU team is composed of 10 undergraduate and graduate students.</br></br><center><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">Meet the Team &#8594;</a></center></font></div>
    <div class="col-sm-4"><font face="Arial" size="6"></br></br>About Us</font><hr></br><font face="Arial" size="3">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. </br></br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">To learn more about our team, click here.</a></font></div>
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<div class="col-sm-1"></div>
    <div class="col-sm-1"></div>
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<div class="col-sm-4"><img src="https://static.igem.org/mediawiki/2017/8/80/Lubbock_TTU_teamphoto.jpg" width="450px"></div>
    <div class="col-sm-4"></br><img src="https://static.igem.org/mediawiki/2017/8/80/Lubbock_TTU_teamphoto.jpg" width="450px"></div>
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<div class="col-sm-1"></div>
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</div>
 
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<div class="col-sm-12"><font face="Arial" size="6"><center>Collaborations</center></font><hr></div>
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<div class="col-sm-4">
<div class="col-sm-10">
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<div class="hover11 column">
</br></br>
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://static.igem.org/mediawiki/2017/9/90/Lubbock_TTU_collaborations.jpg" width="230" height="230"></figure></a>
<font face="Arial" size="6"><center>Human Practices</center></font><hr>
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</br><font face="Arial" size="4">Description 2</font></br></br></br>
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</div>
 
</div>
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</div>
 
</div>
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<div class="hover11 column">
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://www.tacc.utexas.edu/documents/1084364/1085221/utaustin-seal.png/2254124a-1d13-44cc-8e3f-6d925af2391d?t=1412720755000" width="230" height="230"></figure></a>
<div class="col-sm-1"></div>
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<div class="col-sm-10">
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</br></br>
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<font face="Arial" size="6"><center>Collaborations</center></font><hr>
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</br><font face="Arial" size="4">Description 3</font></br></br></br>
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</div>
<div class="col-sm-1"></div>
 
 
</div>
 
</div>
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</br>
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<a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations"><figure><img src="https://static.igem.org/mediawiki/2017/thumb/1/17/T--Erlangen_Nuremberg--Logo.png/240px-T--Erlangen_Nuremberg--Logo.png" width="230" height="230"></figure></a>
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<div class="col-sm-10">
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</br></br>
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<font face="Arial" size="6"><center>InterLab</center></font><hr>
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</br><font face="Arial" size="4">Description 4</font></br></br></br>
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<div class="col-sm-1"></div>
 
 
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<!-- QUICK LINKS -->
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</div> <!-- /container --><!-- end of for NAVI -->
<div style="background-image: url(http://46.105.63.207/client/htmlchat/img/skin/thanksgiving/topcmm-123flashchat-common-main-div-outline-bg.jpg); ; overflow: auto;" class="clear_fix>
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<p align="center><font face="Arial" size="6" color="white"></br></br><center>Quick Links</center></font></p>
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<!-- QL -->
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<style>
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.btn-group button {
<div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Notebook"><i class="fa fa-book" style="font-size:48px;color:white;"></i><font color="white"></br></br>Notebook</font></a></div>
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    background-color: #cc3d3d; /* Green #4CAF50 background */
<div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Parts"><i class="fa fa-cogs" style="font-size:48px;color:white;"></i><font color="white"></br></br>Parts</font></a></div>
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    border: 1px solid #cc3d3d; /* Border color */
<div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Safety"><i class="fa fa-medkit" style="font-size:48px;color:white;"></i><font color="white"></br></br>Safety</font></a></div>
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    color: white; /* White text */
<div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Engagement"><i class="fa fa-spinner" style="font-size:48px;color:white;"></i><font color="white"></br></br>Public Engagement</font></a></div>
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    padding: 10px 24px; /* Some padding */
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/* Add a background color #3e8e41 on hover */
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  <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Notebook"><button style="width:25%"><i class="fa fa-book" style="font-size:45px;color:;"></i></br>Notebook</button></a>
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Latest revision as of 17:38, 12 December 2017



Characterization of a Calcium-Sensitive Reporter in
Saccharomyces cerevisiae



Project Description


Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of In-Silico control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures.

Learn more →




Human Practices


Human Practices is an integral part of creating impactful relationships with our community, and conducting research that is embraced by a generalized audience. The iGEM competition is centered around synthetic biology, of which includes genetic engineering, which traditionally has been a controversial area of research. Careful consideration of Human Practices ensures that our research is ethical, responsible, safe, secure, and sustainable.

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InterLab Study


The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. The objective of the 2017 InterLab Study is to determine absolute units for measuring green fluorescent protein (GFP) that are easily comparable regardless of the type of plate reader used to make the measurement. We used a standardized protocol and analysis method for measuring GFP with a BioTek Synergy 4 plate reader.

View 2017 InterLab Study results →



About Us

The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the Lubbock_TTU team is composed of 10 undergraduate and graduate students.

Meet the Team →



Collaborations