Team:LUBBOCK TTU
For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for In-Silico control in future work. We also built on work from previous iGEM teams to further develop a deterministic model for the calcium pathway in yeast that will activate our reporter genes. Additionally, we developed a stochastic model to predict the nature of ion channel gating in response to temperature change. These models will help guide future work with TRPV1 as a thermal actuator in yeast. To view our results, click here.
(iGEM teams are leading in the area of Human Practices because they conduct their projects within a social/environmental context, to better understand issues that might influence the design and use of their technologies. Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work.)
The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. The objective of the 2017 InterLab Study is to determine absolute units for measuring GFP with a plate reader that are easily comparable despite the type of plate reader used to make the measurement. We used a standardized protocol and analysis method for measuring green fluorescent protein (GFP) with a BioTek Synergy 4 plate reader. To view our results for the 2017 InterLab Study, click here.
About Us
The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. To learn more about our team, click here.
The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. To learn more about our team, click here.
