Difference between revisions of "Team:LUBBOCK TTU/Description"
| Line 96: | Line 96: | ||
<div class="col-sm-4"><img src="https://placehold.it/450x300"></div> | <div class="col-sm-4"><img src="https://placehold.it/450x300"></div> | ||
<div class="col-sm-2"></div> | <div class="col-sm-2"></div> | ||
| − | <div class="col-sm-4"><font face="Arial" size="5"></br><i class="fa fa-wpforms" style="font-size:24px;color:black;"></i> Description</font></br></br><font face="Arial" size="2"> | + | <div class="col-sm-4"><font face="Arial" size="5"></br><i class="fa fa-wpforms" style="font-size:24px;color:black;"></i> Description</font></br></br><font face="Arial" size="2">The most commonly used input signals in iGEM generally include chemicals and light. Temperature-based input signals are not used as frequently and remain a reservoir of potential for future synthetic biology tools. Our project explores the characterization and standardization of thermosensitive ion channels, known as TRPV1, to function as a thermal actuator and regulate calcium-sensitive gene expression in Saccharomyces cerevisiae. At the activation temperature, gating of the thermal actuator allows for an influx of extracellular calcium ions, which initiate the calmodulin-calcineurin signaling pathway, and promote the expression of genes regulated by Crz1p-sensitive promoters. The characterization of the thermal actuator was conducted in S. cerevisiae because yeast offers an accessible eukaryotic chassis for other iGEM teams to work with. By varying the temperature, the thermal actuator can be used to control genetic circuits that function as temperature-sensitive kill switches, drug delivery devices, or biosensors. |
</a></font></div> | </a></font></div> | ||
<div class="col-sm-1"></div> | <div class="col-sm-1"></div> | ||
Revision as of 19:44, 9 September 2017
DescriptionThe most commonly used input signals in iGEM generally include chemicals and light. Temperature-based input signals are not used as frequently and remain a reservoir of potential for future synthetic biology tools. Our project explores the characterization and standardization of thermosensitive ion channels, known as TRPV1, to function as a thermal actuator and regulate calcium-sensitive gene expression in Saccharomyces cerevisiae. At the activation temperature, gating of the thermal actuator allows for an influx of extracellular calcium ions, which initiate the calmodulin-calcineurin signaling pathway, and promote the expression of genes regulated by Crz1p-sensitive promoters. The characterization of the thermal actuator was conducted in S. cerevisiae because yeast offers an accessible eukaryotic chassis for other iGEM teams to work with. By varying the temperature, the thermal actuator can be used to control genetic circuits that function as temperature-sensitive kill switches, drug delivery devices, or biosensors.
What should this page contain?
- A clear and concise description of your project.
- A detailed explanation of why your team chose to work on this particular project.
- References and sources to document your research.
- Use illustrations and other visual resources to explain your project.
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
