Difference between revisions of "Team:LUBBOCK TTU/Model"

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<font face="Arial" size="5">&mdash; Title &mdash;</font></center>
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<font face="Arial" size="5">&mdash; Assumptions &mdash;</font></center>
 
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</br>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.
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</br><b>Assumptions:</b>
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</br>1. Our GFPs’ degradation rate is approximately 25 hours.
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</br>2. Calcium in the cytosol is unable to activate our reporter genes mNeonGreen and sfGFP.
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</br>3. Calcium binds to Calmodulin and all the calcium-bound Calmodulin will bind to Calcineurin to make activated Calcineurin.
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</br>4. Activated Calcineurin will dephosphorylate Crz1p.
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</br>5. Dephosphorylated Crz1p will interact with PMC1 promoter to enable mNeonGreen and sfGFP expression.
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</br>6. Dephosphorylated Crz1p diffuses into the nucleus.
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</br>7.mNeonGreen and sfGFP’s degradation rates are similar to the degradation rate of GFP.
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Revision as of 03:32, 2 November 2017





  Model

(Content)




— Assumptions —

Assumptions:
1. Our GFPs’ degradation rate is approximately 25 hours.
2. Calcium in the cytosol is unable to activate our reporter genes mNeonGreen and sfGFP.
3. Calcium binds to Calmodulin and all the calcium-bound Calmodulin will bind to Calcineurin to make activated Calcineurin.
4. Activated Calcineurin will dephosphorylate Crz1p.
5. Dephosphorylated Crz1p will interact with PMC1 promoter to enable mNeonGreen and sfGFP expression.
6. Dephosphorylated Crz1p diffuses into the nucleus.
7.mNeonGreen and sfGFP’s degradation rates are similar to the degradation rate of GFP.

© 2017 Lubbock_TTU