Team:LUBBOCK TTU/Model

Model

(Content)

— Assumptions —

Assumptions:
1. Our GFPs’ degradation rate is approximately 25 hours.
2. Calcium in the cytosol is unable to activate our reporter genes mNeonGreen and sfGFP.
3. Calcium binds to Calmodulin and all the calcium-bound Calmodulin will bind to Calcineurin to make activated Calcineurin.
4. Activated Calcineurin will dephosphorylate Crz1p.
5. Dephosphorylated Crz1p will interact with PMC1 promoter to enable mNeonGreen and sfGFP expression.
6. Dephosphorylated Crz1p diffuses into the nucleus.
7.mNeonGreen and sfGFP’s degradation rates are similar to the degradation rate of GFP.