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− | {{ | + | {{LUBBOCK_TTU/fontawesome/css/font-awesome-ie7.css}} |
+ | {{LUBBOCK_TTU/fontawesome/css/font-awesome-ie7.min.css}} | ||
+ | {{LUBBOCK_TTU/fontawesome/css/font-awesome.css}} | ||
+ | {{LUBBOCK_TTU/fontawesome/css/font-awesome.min.css}} | ||
<html> | <html> | ||
− | < | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
+ | <link rel="stylesheet" href="https://www.w3schools.com/w3css/4/w3.css"> | ||
− | <link href= | + | <!-- Font Awesome --> |
− | <link href= | + | <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css"> |
− | < | + | <!-- End Font Awesome --> |
− | < | + | <!-- Bootstrap Grid Basic --> |
+ | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css"> | ||
+ | <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.2.1/jquery.min.js"></script> | ||
+ | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> | ||
+ | <!-- End Bootstrap Grid Basic --> | ||
+ | |||
+ | <!--DEFAULT WIKI SETTINGS--> | ||
+ | <style> /* Clear the default wiki settings */ | ||
+ | #home_logo, #sideMenu { display:none; } | ||
+ | #sideMenu, #top_title, .patrollink {display:none;} | ||
+ | #content { width:100%; padding:0px; margin-top:1px; margin-left:0px;} | ||
+ | body {background-color:white; } | ||
+ | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; } | ||
+ | </style> | ||
+ | |||
+ | <!-- TABS CSS --> | ||
+ | <style> | ||
+ | *, *:before, *:after {margin: 0; padding: 0; box-sizing: border-box;} | ||
+ | |||
+ | h1 {padding: 100px 0; font-weight: 400; text-align: center;} | ||
+ | <!--p {margin: 0 0 20px; line-height: 1.5;}--> | ||
+ | |||
+ | .main {margin: 0 auto; min-width: 320px; max-width: 90%;} | ||
+ | .content {background: #fff; color: ##cccccc;} | ||
+ | .content > div {display: none;} | ||
+ | |||
+ | input {display: none;} | ||
+ | label {display: inline-block; padding: 15px 25px; font-weight: 600; text-align: center;} | ||
+ | label:hover {color: #ed5a6a; cursor: pointer;} | ||
+ | input:checked + label {background: #ed5a6a; color: #fff;} | ||
+ | |||
+ | #tab1:checked ~ .content #content1, | ||
+ | #tab2:checked ~ .content #content2, | ||
+ | #tab3:checked ~ .content #content3, | ||
+ | #tab4:checked ~ .content #content4 { | ||
+ | display: block; | ||
+ | } | ||
+ | |||
+ | @media screen and (max-width: 400px) { label {padding: 15px 10px;} } | ||
+ | </style> | ||
+ | <!-- END TABS CSS --> | ||
+ | |||
+ | <!-- FOOTER CSS --> | ||
+ | <style> | ||
+ | /** | ||
+ | * Demo Styles | ||
+ | */ | ||
+ | |||
+ | html { | ||
+ | height: 100%; | ||
+ | box-sizing: border-box; | ||
+ | } | ||
+ | |||
+ | *, | ||
+ | *:before, | ||
+ | *:after { | ||
+ | box-sizing: inherit; | ||
+ | } | ||
+ | |||
+ | body { | ||
+ | position: relative; | ||
+ | margin: 0; | ||
+ | <!--padding-bottom: 6rem;--> | ||
+ | min-height: 100%; | ||
+ | } | ||
+ | |||
+ | /** | ||
+ | * Footer Styles | ||
+ | */ | ||
+ | |||
+ | .footer { | ||
+ | position: absolute; | ||
+ | right: 0; | ||
+ | bottom: -900; | ||
+ | left: 0; | ||
+ | padding: 1rem; | ||
+ | background-color: #fff; | ||
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+ | <!-- END FOOTER CSS --> | ||
<body> | <body> | ||
− | < | + | <center> |
− | < | + | <div style="width:1110px;" style="text-align: center;> |
− | < | + | <div class="w3-bar w3-light-grey"> |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HOME</font></a> | |
− | + | <div class="w3-dropdown-hover"> | |
− | + | <button class="w3-button"><font face="Arial" size="2" color="black">TEAM</font></button> | |
− | + | <div class="w3-dropdown-content w3-bar-block w3-card-4"> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">TEAM</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Collaborations" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">COLLABORATIONS</font></a> | |
− | + | </div> | |
− | + | </div> | |
− | </ | + | <div class="w3-dropdown-hover"> |
− | + | <button class="w3-button"><font face="Arial" size="2" color="black">PROJECT</font></button> | |
− | < | + | <div class="w3-dropdown-content w3-bar-block w3-card-4"> |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESCRIPTION</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DESIGN</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Experiments" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">EXPERIMENTS</font></a> | |
− | </ | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Notebook" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">NOTEBOOK</font></a> |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/InterLab" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTERLAB</font></a> | |
− | < | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Contribution" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">CONTRIBUTION</font></a> |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Results" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">RESULTS</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Demonstrate" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">DEMONSTRATE</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Improve" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">IMPROVE</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Attributions" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ATTRIBUTIONS</font></a> | |
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− | + | </div> | |
− | + | <div class="w3-dropdown-hover"> | |
− | + | <button class="w3-button"><font face="Arial" size="2" color="black">PARTS</font></button> | |
− | + | <div class="w3-dropdown-content w3-bar-block w3-card-4"> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Parts" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PARTS</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Basic_Part" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">BASIC PARTS</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Composite_Part" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">COMPOSITE PARTS</font></a> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Part_Collection" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PART COLLECTION</font></a> | |
− | + | </div> | |
− | + | </div> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Safety" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SAFETY</font></a> | |
− | + | <div class="w3-dropdown-hover"> | |
− | + | <button class="w3-button"><font face="Arial" size="2" color="black">HUMAN PRACTICES</font></button> | |
− | + | <div class="w3-dropdown-content w3-bar-block w3-card-4"> | |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Silver" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SILVER HP</font></a> | |
− | < | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/HP/Gold_Integrated" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">INTEGRATED AND GOLD</font></a> |
− | + | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Engagement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PUBLIC ENGAGEMENT</font></a> | |
− | </ | + | </div> |
− | < | + | </div> |
− | < | + | <div class="w3-dropdown-hover"> |
− | < | + | <button class="w3-button"><font face="Arial" size="2" color="black">AWARDS</font></button> |
+ | <div class="w3-dropdown-content w3-bar-block w3-card-4"> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Applied_Design" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">APPLIED DESIGN</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Entrepreneurship" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">ENTREPRENEURSHIP</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Hardware" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">HARDWARE</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Measurement" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MEASUREMENT</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Model" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">MODEL</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Plant" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">PLANT</font></a> | ||
+ | <a href="https://2017.igem.org/Team:LUBBOCK_TTU/Software" class="w3-bar-item w3-button"><font face="Arial" size="2" color="black">SOFTWARE</font></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </center> | ||
− | <!-- | + | <!-- HEADER --> |
+ | </br><div style="background-image: url(https://placehold.it/1120x570);> | ||
+ | <p align="center><font face="Arial" size="6" color="white"> | ||
+ | <center></br>Characterization of a Thermal</br>Actuator and Calcium-Sensitive Reporter in</br>Saccharomyces cerevisiae</br></br></center></font></p> | ||
+ | </div> | ||
− | <!-- | + | <!-- END HEADER--> |
+ | <!-- PROJECT DESCRIPTION --> | ||
+ | <div style="background-color:#fff; overflow: auto;" class="clear_fix"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-10"> | ||
+ | </br></br> | ||
+ | <font face="Arial" size="6"><center>Project Description</center></font><hr> | ||
+ | </br><font face="Arial" size="4"> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-sm-6"><font face="Arial" size="4">Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of <i>In-Silico</i> control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures. | ||
+ | </br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">To learn more about our project, click here.</a></font></div> | ||
+ | <div class="col-sm-6"><img src="http://via.placeholder.com/450x300"> | ||
+ | </div></br> | ||
+ | |||
+ | </font></br> | ||
+ | </div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <!-- END PROJECT DESCRIPTION --> | ||
+ | |||
+ | <!-- PROJECT AIMS --> | ||
</br> | </br> | ||
+ | <div style="background-color:#fff; overflow: auto;" class="clear_fix"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-10"> | ||
+ | </br></br> | ||
+ | <font face="Arial" size="6"><center>Project Aims</center></font><hr> | ||
+ | </br><font face="Arial" size="4">For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for <i>In-Silico</i> control in future work. | ||
+ | |||
+ | </br></br>We also built on work from previous iGEM teams to further develop a deterministic model for the calcium pathway in yeast that will activate our reporter genes. Additionally, we developed a stochastic model to predict the nature of ion channel gating in response to temperature change. These models will help guide future work with TRPV1 as a thermal actuator in yeast. | ||
+ | |||
+ | </br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Results">To view our results, click here.</a> | ||
+ | </font></br> | ||
+ | </div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | </div> | ||
+ | <!-- END PROJECT AIMS --> | ||
+ | |||
+ | <!-- HP --> | ||
+ | <div style="background-color:#fff; overflow: auto;" class="clear_fix"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-10"> | ||
+ | </br></br> | ||
+ | <font face="Arial" size="6"><center>Human Practices</center></font><hr> | ||
+ | </br><font face="Arial" size="4">Description 2</font></br></br></br> | ||
+ | </div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | </div> | ||
+ | <!-- END HP --> | ||
+ | |||
+ | <!-- COLLABS --> | ||
+ | </br> | ||
+ | <div style="background-color:#fff; overflow: auto;" class="clear_fix"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-10"> | ||
+ | </br></br> | ||
+ | <font face="Arial" size="6"><center>Collaborations</center></font><hr> | ||
+ | </br><font face="Arial" size="4">Description 3</font></br></br></br> | ||
+ | </div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | </div> | ||
+ | <!-- END COLLABS --> | ||
+ | |||
+ | |||
+ | <!-- INTERLAB --> | ||
+ | </br> | ||
+ | <div style="background-color:#fff; overflow: auto;" class="clear_fix"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-10"> | ||
+ | </br></br> | ||
+ | <font face="Arial" size="6"><center>InterLab</center></font><hr> | ||
+ | </br><font face="Arial" size="4">Description 4</font></br></br></br> | ||
+ | </div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | </div> | ||
+ | <!-- END INTELRAB --> | ||
+ | |||
+ | <!-- ABOUT US --> | ||
+ | <div class="container-fluid"> | ||
+ | </br> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-4"><font face="Arial" size="6"></br></br>About Us</font><hr></br><font face="Arial" size="3">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. </br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">To learn more about our team, click here.</a></font></div> | ||
+ | <div class="col-sm-1"></div> | ||
+ | <div class="col-sm-4"></br></br><img src="https://static.igem.org/mediawiki/2017/8/80/Lubbock_TTU_teamphoto.jpg" width="450px"></div> | ||
+ | </div></br></br> | ||
+ | </div> | ||
+ | <!-- END ABOUT US --> | ||
+ | |||
+ | <!-- QUICK LINKS --> | ||
+ | <div style="background-image: url(http://46.105.63.207/client/htmlchat/img/skin/thanksgiving/topcmm-123flashchat-common-main-div-outline-bg.jpg); ; overflow: auto;" class="clear_fix> | ||
+ | <p align="center><font face="Arial" size="6" color="white"></br></br><center>Quick Links</center></font></p> | ||
+ | </br> | ||
+ | <div class="col-sm-2"></div><center> | ||
+ | <div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Notebook"><i class="fa fa-book" style="font-size:48px;color:white;"></i><font color="white"></br></br>Notebook</font></a></div> | ||
+ | <div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Parts"><i class="fa fa-cogs" style="font-size:48px;color:white;"></i><font color="white"></br></br>Parts</font></a></div> | ||
+ | <div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Safety"><i class="fa fa-medkit" style="font-size:48px;color:white;"></i><font color="white"></br></br>Safety</font></a></div> | ||
+ | <div class="col-sm-2"><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Engagement"><i class="fa fa-spinner" style="font-size:48px;color:white;"></i><font color="white"></br></br>Public Engagement</font></a></div> | ||
+ | </br></br></br></br></br> | ||
+ | </center><div class="col-sm-2"></div> | ||
+ | </div> | ||
+ | <!-- END QUICK LINKS --> | ||
+ | |||
+ | <!-- SECTION 4 --> | ||
+ | <div style="background-image: url(http://p1.pichost.me/i/56/1792614.jpg); ; overflow: auto;" class="clear_fix> | ||
+ | <p align="center><font face="Arial" size="6" color="white"></br></br><center>Our Sponsors</center></font></p> | ||
+ | <div class="col-sm-2"></br><img src="https://static.igem.org/mediawiki/2017/e/e4/Lubbock_TTU_artsandsciences.png" height="" width="230"></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"></br><img src="https://static.igem.org/mediawiki/2016/5/51/Muc16_Sponsor_IDT.png" height="" width="200"></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"></br><img src="https://static.igem.org/mediawiki/2014/5/5a/York_Genscript_logo-1-.png" height="" width="200"></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"><img src="http://www.mrdnalab.com/images/logobig330x142.png?crc=3839258444" height="" width="200"></div> | ||
+ | </br></br></br> | ||
+ | <div class="col-sm-2"></br></br></br><img src="https://static1.squarespace.com/static/54e7602be4b0165bdcece115/t/585aa64d6a49639e7a53948b/1482335827516/ttuhsc.png" height="" width="200"></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"></br></br><img src="https://static.igem.org/mediawiki/2017/c/c4/Lubbock_TTU_troemner.png" height="120" width=""></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"></br></br><img src="https://static.igem.org/mediawiki/2016/a/ad/T--Lubbock_TTU--ulabs.png" height="150" width=""></div> | ||
+ | <div class="col-xs-1"></div> | ||
+ | <div class="col-sm-2"></br></br></br></br><img src="https://upload.wikimedia.org/wikipedia/commons/7/70/Whitacre_College_of_Engineering_logo.svg" height="" width="220"></div> | ||
+ | </br></br></br> | ||
+ | <div class="col-sm-12"></br></br></div> | ||
+ | </div> | ||
+ | <!-- END SECTION 4 --> | ||
</body> | </body> | ||
+ | |||
+ | <!-- FOOTER --> | ||
+ | <div class="footer"><center><font face="Arial" size="3" color="black">© 2017 Lubbock_TTU</font></center></div> | ||
+ | <!-- END FOOTER --> | ||
</html> | </html> |
Revision as of 16:37, 21 October 2017
Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of In-Silico control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures.
To learn more about our project, click here.
For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for In-Silico control in future work. We also built on work from previous iGEM teams to further develop a deterministic model for the calcium pathway in yeast that will activate our reporter genes. Additionally, we developed a stochastic model to predict the nature of ion channel gating in response to temperature change. These models will help guide future work with TRPV1 as a thermal actuator in yeast. To view our results, click here.
Description 2
Description 3
Description 4
About Us
The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. To learn more about our team, click here.
The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. To learn more about our team, click here.