Team:Macquarie Australia/Improve
We have improved the characterisation of the previously submitted part BBa_K1998011. This was submitted by the Macquarie University iGEM team of 2016 and contains the Chlamydomonas reinhardtii Ferredoxin protein and Ferredoxin NADP+ reductase.
We first induced operon expression with (how many mM) IPTG in Escherichia coli DH5α. The cells were then centrifuged at 14,000 xg to lyse them, and anionic exchange chromatography was performed on the lysate using a Source Q column. The purified proteins were observed from the pronounced colour change in the fractions, as can be seen in Figure 1.
GOLD SPONSORS |
BRONZE SPONSORS |
LOCATIONMacquarie University Balaclava Road, North Ryde, NSW, 2109, Australia E7B 350 CONTACT US |
FOLLOW US ON
|
