Difference between revisions of "Team:Macquarie Australia/Validation"
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They quote - "IPTG and 20mM of glucose was used to induce the gene expression. Comparison of 1mM IPTG and 20mM IPTG was made and it was found that 1mM IPTG has higher gene expression level. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products." <a href="https://2017.igem.org/Team:NTU_SINGAPORE/Collaborations"> - NTU Singapore </a> | They quote - "IPTG and 20mM of glucose was used to induce the gene expression. Comparison of 1mM IPTG and 20mM IPTG was made and it was found that 1mM IPTG has higher gene expression level. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products." <a href="https://2017.igem.org/Team:NTU_SINGAPORE/Collaborations"> - NTU Singapore </a> | ||
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<img src="https://static.igem.org/mediawiki/2017/9/95/Graph_KM.jpg"> | <img src="https://static.igem.org/mediawiki/2017/9/95/Graph_KM.jpg"> | ||
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This validation from Singapore helped clarify that all of the genes in our Hydrogen Gas Producing Gene Cluster were expressing. Albeit, with HydG expressing less for some unknown reason. | This validation from Singapore helped clarify that all of the genes in our Hydrogen Gas Producing Gene Cluster were expressing. Albeit, with HydG expressing less for some unknown reason. | ||
Revision as of 13:21, 31 October 2017
Hydrogen Gas Producing Gene Cluster - HGPC
Our Validation Work
NTU Singapore's Validation
This year again, we sought assistance from team NTU Singapore. They helped us to evaluate and validate the gene expression level of each of the genes in our Hydrogen Gas Producing Gene Cluster, by using RT-PCR.
They quote - "IPTG and 20mM of glucose was used to induce the gene expression. Comparison of 1mM IPTG and 20mM IPTG was made and it was found that 1mM IPTG has higher gene expression level. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products." - NTU Singapore
This validation from Singapore helped clarify that all of the genes in our Hydrogen Gas Producing Gene Cluster were expressing. Albeit, with HydG expressing less for some unknown reason.
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