Difference between revisions of "Team:Munich"

 
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href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
<style>
 
<style>
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line-height: initial !important;
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body {line-height: initial;}
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h1, h2,h3 {
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color: #51a7f9 !important;
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text-align: center !important;
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}
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/*Time for the CSS*/
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* {margin: 0; padding: 0;}
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.slider{
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width: 900px; /*Same as width of the large image*/
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position: relative;
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/*Instead of height we will use padding*/
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padding-top: 600px; /*That helps bring the labels down*/
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/*Lets add a shadow*/
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}
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/*Last thing remaining is to add transitions*/
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.slider>img{
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position: absolute;
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left: 0; top: 0;
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transition: all 0.5s;
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}
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.slider input[name='slide_switch'] {
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display: none;
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}
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.slider label {
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/*Lets add some spacing for the thumbnails*/
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margin: 30px 0 0 18px;
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float: left;
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cursor: pointer;
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transition: all 0.5s;
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/*Default style = low opacity*/
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opacity: 0.6;
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        z-index: 99;
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}
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.slider label img{
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display: block;
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}
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/*Time to add the click effects*/
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.slider input[name='slide_switch']:checked+label {
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border-color: #666;
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opacity: 1;
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}
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/*Clicking any thumbnail now should change its opacity(style)*/
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/*Time to work on the main images*/
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.slider input[name='slide_switch'] ~ img {
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opacity: 0;
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transform: scale(1.1);
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}
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/*That hides all main images at a 110% size
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On click the images will be displayed at normal size to complete the effect
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*/
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.slider input[name='slide_switch']:checked+label+img {
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opacity: 1;
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transform: scale(1);
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}
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/*Clicking on any thumbnail now should activate the image related to it*/
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/*We are done :)*/
 
</style>
 
</style>
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</head><body>
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</head></html>
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{{Munich/Menu}}
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<html>
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<body>
 
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
 
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
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<tr><td height="50" bgcolor=#ffffff></td><td class="no-padding" bgcolor=#ffffff height="32" width="960" align=center>
 
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<div class="dropdown">
 
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  <a href="/Team:Munich"><button class="dropbtnCascAID dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></button></a>
 
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</div><div class="dropdown">
 
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  <a href="project"><button class="dropbtnProject dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Project</b></p></button></a>  <div class="dropdown-contentDescription dropdown-content">
 
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    <a href="description"><p><b>Description</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentDesign dropdown-content">
 
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    <a href="design"><p><b>Design</b></p></a>
 
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  <div class="dropdown-contentProofofConcept dropdown-content">
 
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    <a href="proofofconcept"><p><b>Proof of Concept</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentDemonstration dropdown-content">
 
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    <a href="demonstration"><p><b>Demonstration</b></p></a>
 
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  <div class="dropdown-contentApplication dropdown-content">
 
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    <a href="application"><p><b>Application</b></p></a>
 
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  <div class="dropdown-contentFinalResults dropdown-content">
 
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    <a href="finalresults"><p><b>Final Results</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentEntrepreneurship dropdown-content">
 
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    <a href="entrepreneurship"><p><b>Entrepeneurship</b></p></a>
 
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  <div class="dropdown-contentAchievements dropdown-content">
 
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    <a href="achievements"><p><b>Achievements</b></p></a>
 
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  </div>
 
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</div><div class="dropdown" valign=center>
 
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<a href="wetlab"><button class="dropbtnWetlab dropbtn" valign=center><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Wetlab</b></p></button></a>
 
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  <div class="dropdown-contentProtocolsMethods dropdown-content">
 
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    <a href="protocolsmethods"><p><b>Protocols and<br> Methods</b></p></a>
 
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  <div class="dropdown-contentMaterials dropdown-content">
 
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    <a href="materials"><p><b>Materials</b></p></a>
 
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  <div class="dropdown-contentLabJournal dropdown-content">
 
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    <a href="labjournal"><p><b>LabJournal</b></p></a>
 
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  <div class="dropdown-contentSafety dropdown-content">
 
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    <a href="safety"><p><b>Safety</b></p></a>
 
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  <div class="dropdown-contentParts dropdown-content">
 
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    <a href="parts"><p><b>Parts</b></p></a>
 
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  <div class="dropdown-contentInterlab dropdown-content">
 
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    <a href="interlab"><p><b>Interlab</b></p></a>
 
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  </div>
 
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</div><div class="dropdown">
 
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  <a href="model"><button class="dropbtnModel dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Modelling</b></p></button></a></div><div class="dropdown">
 
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  <a href="softhardware"><button class="dropbtnSoftHardware dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Software/Hardware</b></p></button></a>
 
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  <div class="dropdown-contentSoftware dropdown-content">
 
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    <a href="software"><p><b>Software</b></p></a>
 
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  <div class="dropdown-contentHardware dropdown-content">
 
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    <a href="hardware"><p><b>Hardware</b></p></a>
 
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</div><div class="dropdown">
 
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  <a href="hp"><button class="dropbtnHP dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Human Practices</b></p></button></a>
 
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  <div class="dropdown-contentHPSilver dropdown-content">
 
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    <a href="hpsilver"><p><b>Human Practices (Silver)</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentIHPgold dropdown-content">
 
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    <a href="ihpgold"><p><b>Human Practices (Gold)</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentPublicEngagement dropdown-content">
 
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    <a href="publicengagement"><p><b>Public Engagement</b></p></a>
 
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  <div class="dropdown-contentCollaborations dropdown-content">
 
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    <a href="collaborations"><p><b>Collaborations</b></p></a>
 
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  </div>
 
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</div><div class="dropdown">
 
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  <a href="/Team:Munich/Team"><button class="dropbtnTeam dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Team</b></p></button></a>
 
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  <div class="dropdown-contentTeamMembers dropdown-content">
 
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    <a href="/Team:Munich/Team"><p><b>Team members</b></p></a>
 
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  <div class="dropdown-contentSupervisors dropdown-content">
 
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    <a href="supervisors"><p><b>Supervisors</b></p></a>
 
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  <div class="dropdown-contentPIs dropdown-content">
 
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    <a href="supervisors"><p><b>PIs</b></p></a>
 
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  <div class="dropdown-contentCollaboratingInstitutions dropdown-content">
 
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    <a href="supervisors"><p><b>Institutions</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentSponsors dropdown-content">
 
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    <a href="sponsors"><p><b>Sponsors</b></p></a>
 
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  </div>
 
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  <div class="dropdown-contentAttributions dropdown-content">
 
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    <a href="/Team:Munich/Team"><p><b>Attributions</b></p></a>
 
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  </div>
 
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</div>
 
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</td><td height="32" bgcolor=#ffffff></td></tr>
 
 
<!-- Content -->
 
<!-- Content -->
 
<tr><td width="100%" colspan=3>
 
<tr><td width="100%" colspan=3>
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<!-- Head End -->
 
<!-- Head End -->
 
<!-- Content Begin -->
 
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<video height="270" width="960" autoplay="autoplay">
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<video width="980" autoplay="autoplay" controls>
 
<source src="https://static.igem.org/mediawiki/2017/4/44/T--Munich--FrontPageVideo.mp4" type="video/mp4">
 
<source src="https://static.igem.org/mediawiki/2017/4/44/T--Munich--FrontPageVideo.mp4" type="video/mp4">
 
Your browser does not support the video tag.
 
Your browser does not support the video tag.
 
</video>
 
</video>
 
<table width="900" border=0 cellspacing=0 cellpadding=10>
 
<table width="900" border=0 cellspacing=0 cellpadding=10>
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<tr><td colspan=2 align=left valign=center>
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<tr>
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<font size=7 color=#444444><b>Abstract</b></font>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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</tr>
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<tr><td colspan=6 align=left valign=center>
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<font size=7 ><b style="color: #51a7f9">Abstract</b></font>
 
</td></tr>
 
</td></tr>
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<tr><td align=center valign=center>
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<tr><td align=center valign=center colspan=6>
 
<p style="text-align:justify">
 
<p style="text-align:justify">
 
<font size=3 color=#444444>
 
<font size=3 color=#444444>
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The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
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The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from <i>E. coli</i>. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
 
</font>
 
</font>
 
</p>
 
</p>
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<p style="text-align:justify; margin-bottom: 50px">
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</td><td align=center valign=center>
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<img src="https://static.igem.org/mediawiki/2017/thumb/a/af/T--Munich--Logo_Final.svg/739px-T--Munich--Logo_Final.svg.png" width="420">
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<p style="text-align:justify">
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<font size=3 color=#444444>
 
<font size=3 color=#444444>
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
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</font>
 
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<font size=3 color=#444444>
 
 
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
</font>
 
</font>
 
</p>
 
</p>
 
</td></tr>
 
</td></tr>
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<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
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<tr><td colspan=6 align=middle valign=center>
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<font size=2>| The iGEM Munich 2017 Team | 2017.07.13 |</font><br><br><br><center><hr></center>
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<font size=10 ><b style="color: #51a7f9; font-size: 60px; margin-top: 20px">Awards & Achievements</b></font>
 
</td></tr>
 
</td></tr>
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<tr>
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<!-- Content End -->
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<img src="https://static.igem.org/mediawiki/2017/e/e5/T--Munich--Banner_Awards.jpg" width=900>
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<table width=300 height=35 cellpadding=0 cellspacing=0 border=0><tr>
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<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
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<img src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_coop_lmu_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg'">
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</a>
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</td>
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</tr>
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<td align=center valign=center>
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<tr>
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<a href="https://www.tum.de/" target="_blank4" >
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<td align=center valign=center colspan=3>
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<img src="https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/1/1e/T--Munich--wiki_image_coop_tum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg'">
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<div class="captionPicture">
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</a>
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<h1>First Runner-up</h1>
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<div class="captionPicture">
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<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
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<h1>Best Diagnostics Project</h1>
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<img src="https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/72/T--Munich--wiki_image_coop_e14_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg'">
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<a href="http://www.biochem.mpg.de/en" target="_blank5" >
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<img src="https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg'">
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<div class="captionPicture">
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<h2>Best Model</h2>
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<div class="captionPicture">
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<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
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<h2>Best Applied Design</h2>
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<img src="https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6b/T--Munich--wiki_image_coop_helmholtzzentrum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg'">
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<a href="https://igem.org/Main_Page" target="_blank6" >
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<img src="https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_coop_igem_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg'">
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<h2>Best Hardware</h2>
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</tr></table></td><td class="no-padding" align=center valign=center><table width=35 height=35 cellpadding=0 cellspacing=0 border=0><tr><td width="35" align=center valign=center></td></tr></table></td><td class="no-padding" align=left valign=center><table width=200 height=35 cellpadding=0 cellspacing=0 border=0><tr>
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<h2>Best Software</h2>
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<a href="https://www.facebook.com/Munich.iGEM" target="_blank7" >
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<img src="https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/4/44/T--Munich--wiki_image_sm_facebook_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg'">
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<a href="mailto:igem.munich@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/9/93/T--Munich--wiki_image_sm_mail_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg'">
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<h3>Nominated for Best Wiki</h3>
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</a>
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<h3>Nominated for Best Presentation</h3>
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<a href="https://www.instagram.com/igem.munich.2017/" target="_blank9" >
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<img src="https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6e/T--Munich--wiki_image_sm_instagram_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg'">
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<h3>Nominated for Best Poster</h3>
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<a href="https://2017.igem.org/Team:Munich" target="_blank10" >
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Latest revision as of 20:49, 15 December 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics. Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

Awards & Achievements

First Runner-up

Best Diagnostics Project

Best Model

Best Applied Design

Best Hardware

Best Software

Nominated for Best Wiki

Nominated for Best Presentation

Nominated for Best Poster

Nominated for Best Integrated Human Practices

Nominated for Best Education & Public Engagement

Nominated for Best Measurement

Nominated for Best Composite Part

Nominated for Best Part Collection
Gallery

See the rest of our gallery...